Sucrose phosphorylase as cross-linked enzyme aggregate: Improved thermal stability for industrial applications

Biotechnology Journal

Volumn 5, Issue 11, 2010, Pages 1192-1197

  Cerdobbel, An ^a   De Winter, Karel ^a   Desmet, Tom ^a   Soetaert, Wim ^a  

^a GHENT UNIVERSITY   (Belgium)

Author keywords

Biocatalysis; Cross linked enzyme aggregate; Enzyme immobilization; Sucrose phosphorylase; Thermostability

Indexed keywords

BIFIDOBACTERIUM ADOLESCENTIS; BIOCATALYSIS; COMMERCIAL POTENTIAL; CONSECUTIVE REACTION; CROSS-LINKED ENZYME AGGREGATES; DONOR SUBSTRATES; IMMOBILIZED ENZYME; MICROBIAL CONTAMINATION; SMALL MOLECULES; SOLUBLE ENZYMES; SUCROSE PHOSPHORYLASE; THERMAL STABILITY; THERMOSTABILITY;

AGGREGATES; ENZYME ACTIVITY; ENZYMES; INDUSTRIAL APPLICATIONS; PHOSPHORYLATION; SUGAR (SUCROSE);

ENZYME IMMOBILIZATION;

SUCROSE; SUCROSE PHOSPHORYLASE; UNCLASSIFIED DRUG;

ARTICLE; BIFIDOBACTERIUM ADOLESCENTIS; BIOCATALYST; ENZYME ACTIVITY; ENZYME IMMOBILIZATION; GLYCOSYLATION; HUMAN; INCUBATION TEMPERATURE; INDUSTRIAL AREA; MICROBIAL CONTAMINATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN CROSS LINKING; REACTION TIME; THERMOSTABILITY;

BIFIDOBACTERIUM; ENZYME STABILITY; ENZYMES, IMMOBILIZED; GLUCOSYLTRANSFERASES;

BIFIDOBACTERIUM ADOLESCENTIS;

EID: 78349258236     PISSN: 18606768     EISSN: 18607314     Source Type: Journal    
DOI: 10.1002/biot.201000202     Document Type: Article

References (30)

1. Sheldon, R. A., Enzyme immobilization: The quest for optimum performance. Adv. Synth. Catal. 2007, 349, 1289-1307.
2. Brady, D., Jordaan, J., Advances in enzyme immobilisation. Biotechnol. Lett. 2009, 31, 1639-1650.
3. Hanefeld, U., Gardossi, L., Magner, E., Understanding enzyme immobilisation. Chem. Soc. Rev. 2009, 38, 453-468.
4. Bornscheuer, U. T., Immobilizing enzymes: How to create more suitable biocatalysts. Angew. Chem. Int. Ed. 2003, 42, 3336-3337.
5. Cao, L. Q., van Rantwijk, F., Sheldon, R. A., Cross-linked enzyme aggregates: A simple and effective method for the immobilization of penicillin acylase. Org. Lett. 2000, 2, 1361-1364.
6. Cao, L. Q., van Langen, L., Sheldon, R. A., Immobilised enzymes: Carrier-bound or carrier-free? Curr. Opin. Biotechnol. 2003, 14, 387-394.
7. Tischer, W., Kasche, V., Immobilized enzymes: Crystals or carriers? Trends Biotechnol. 1999, 17, 326-335.
8. Abraham, T. E., Joseph, J. R., Bindhu, L. B. V., Jayakumar, K. K., Crosslinked enzyme crystals of glucoamylase as a potent catalyst for biotransformations. Carbohydr. Res. 2004, 339, 1099-1104.
9. Lee, J. H., Yoon, S. H., Nam, S. H., Moon, Y. H. et al., Molecular cloning of a gene encoding the sucrose phosphorylase from Leuconostoc mesenteroides B-1149 and the expression in Escherichia coli. Enzyme Microb. Technol. 2006, 39, 612-620.
10. Henrissat, B., A classification of glycosyl hydrolases based on amino-acid-sequence similarities. Biochem. J. 1991, 280, 309-316.
11. Goedl, C., Nidetzky, B., Sucrose phosphorylase harbouring a redesigned, glycosyltransferase-like active site exhibits retaining glucosyl transfer in the absence of a covalent intermediate. ChemBioChem 2009, 10, 2333-2337.
12. Sprogoe, D., van den Broek, L. A. M., Mirza, O., Kastrup, J. S. et al., Crystal structure of sucrose phosphorylase from Bifidobacterium adolescentis. Biochemistry 2004, 43, 1156-1162.
13. Tedokon, M., Suzuki, K., Kayamori, Y., Fujita, S. et al., Enzymatic assay of inorganic phosphate with use of sucrose phosphorylase and phosphoglucomutase. Clin. Chem. 1992, 38, 512-515.
14. Birnberg, P. R., Brenner, M. L., A one-step enzymatic assay for sucrose with sucrose phosphorylase. Anal. Biochem. 1984, 142, 556-561.
15. Goedl, C., Schwarz, A., Minani, A., Nidetzky, B., Recombinant sucrose phosphorylase fromLeuconostoc mesenteroides: Characterization, kinetic studies of transglucosylation, and application of immobilised enzyme for production of alpha-D-glucose 1-phosphate. J. Biotechnol. 2007, 129, 77-86.
16. Kitao, S., Matsudo, T., Saitoh, M., Horiuchi, T. et al., Enzymatic synthesis of 2 stable (-)-epigallocatechin gallate-glucosides by sucrose phosphorylase. Biosci. Biotechnol. Biochem. 1995, 59, 2167-2169.
17. Sawangwan, T., Goedl, C., Nidetzky, B., Single-step enzymatic synthesis of (R)-2-O-alpha-D-glucopyranosyl glycerate, a compatible solute from micro-organisms that functions as a protein stabiliser. Org. Biomol. Chem. 2009, 7, 4267-4270.
18. Goedl, C., Sawangwan, T., Mueller, M., Schwarz, A. et al., A high-yielding biocatalytic process for the production of 2-O-(alpha-D-glucopyranosyl)-sn-glycerol, a natural osmolyte and useful moisturizing ingredient. Angew. Chem. Int. Ed. 2008, 47, 10086-10089.
19. Goedl, C., Sawangwan, T., Wildberger, P., Nidetzky, B., Sucrose phosphorylase: A powerful transglucosylation catalyst for synthesis of alpha-D-glucosides as industrial fine chemicals. Biocatal. Biotransformation 2010, 28, 10-21.
20. Bruins, M. E., Janssen, A. E. M., Boom, R. M., Thermozymes and their applications-A review of recent literature and patents. Appl. Biochem. Biotechnol. 2001, 90, 155-186.
21. Vieille, C., Zeikus, J. G., Thermozymes: Identifying molecular determinants of protein structural and functional stability. Trends Biotechnol. 1996, 14, 183-190.
22. Eijsink, V. G. H., Gaseidnes, S., Borchert, T. V., van den Burg, B., Directed evolution of enzyme stability. Biomol. Eng. 2005, 22, 21-30.
23. De Mey, M., Maertens, J., Lequeux, G. J., Soetaert, W. K. et al., Construction and model-based analysis of a promoter library for E. coli: An indispensable tool for metabolic engineering. BCM Biotechnol. 2007, 18, 34-39.
24. Waffenschmidt, S., Jaenicke, L., Assay of reducing sugars in the nanomole range with 2, 2'-bicinchoninate. Anal. Biochem. 1987, 165, 337-340.
25. Werner, W., Rey, H. G., Wielinge, H, Properties of a new chromogen for determination of glucose in blood according to god/pod-method. Z. Anal. Chem. 1970, 252, 224.
26. Wilson, L., Manes, A., Soler, L., Henriquez, M. J., Effect of the degree of cross-linking on the properties of different CLEAs of penicillin acylase. Process Biochem. 2009, 44, 322-326.
27. Mateo, C., Palomo, J. M., van Langen, L. M., van Rantwijk, F. et al., A new, mild cross-linking methodology to prepare cross-linked enzyme aggregates. Biotechnol. Bioeng. 2004, 86, 273-276.
28. Clark, D. S., Can immobilization be exploited to modify enzyme-activity. Trends Biotechnol. 1994, 12, 439-443.
29. Mateo, C., Grazu, V., Pessela, B. C. C., Montes, T. et al., Advances in the design of new epoxy supports for enzyme immobilization-stabilization. Biochem. Soc. Trans. 2007, 35, 1593-1601.
30. Aerts, D., Verhaeghe, T., De Mey, M., Desnet, T., Soetaert, N. et al., A constitutive expression system for high-throughput screening. Eng. Life Sci. 2010, DOI: 10.1002/elsc.201000065