A stable system for the high-titer production of multiply attenuated lentiviral vectors

Molecular Therapy

Volumn 2, Issue 2, 2000, Pages 170-176

  Klages, Natacha ^a   Zufferey, Romain ^a   Trono, Didier ^a,b  

^a UNIVERSITY OF GENEVA   (Switzerland)

^b University Medical Center   (Switzerland)

Author keywords

Gene therapy; HIV based vectors; Lentiviral vectors; Nondividing cells; Packaging cell line; Retroviral vectors; Transduction; VSV G pseudotypes

Indexed keywords

HUMAN IMMUNODEFICIENCY VIRUS 1; VESICULAR STOMATITIS VIRUS;

G PROTEIN, VESICULAR STOMATITIS VIRUS; MEMBRANE PROTEIN; RNA DIRECTED DNA POLYMERASE; VIRUS ENVELOPE PROTEIN;

ARTICLE; CELL LINE; CELL SEPARATION; FLOW CYTOMETRY; GENE THERAPY; GENE VECTOR; GENETIC TRANSDUCTION; GENETIC TRANSFECTION; GENETICS; HELA CELL; HUMAN; HUMAN IMMUNODEFICIENCY VIRUS; KINETICS; LENTIVIRINAE; METABOLISM; METHODOLOGY; PLASMID; TIME; WESTERN BLOTTING;

BLOTTING, WESTERN; CELL LINE; CELL SEPARATION; FLOW CYTOMETRY; GENE THERAPY; GENETIC VECTORS; HELA CELLS; HIV; HUMANS; KINETICS; LENTIVIRUS; MEMBRANE GLYCOPROTEINS; PLASMIDS; RNA-DIRECTED DNA POLYMERASE; TIME FACTORS; TRANSDUCTION, GENETIC; TRANSFECTION; VIRAL ENVELOPE PROTEINS;

EID: 0034244658     PISSN: 15250016     EISSN: None     Source Type: Journal    
DOI: 10.1006/mthe.2000.0103     Document Type: Article

References (17)

1. Naldini, L., et al. (1996). In vivo gene delivery and stable transduction of nondividing cells by a lentiviral vector. Science 272: 263-267.
2. Carroll, R., Lin, J. T., Dacquel, E. J., Mosca, J. D., Burke, D. S., and St. Louis, D. C. (1994). A human immunodeficiency virus tape 1 (HIV-1)-based retroviral vector system utilizing stable HIV-1 packaging cell lines. J. Virol. 68: 6047-6051.
3. Yu, H., Rabson, A. B., Kaul, M., Ron, Y., and Dougherty, J. P. (1996). Inducible human immunodeficiency virus type 1 packaging cell lines. J. Virol. 70: 4530-4537.
4. Kaul, M., Yu, H., and Dougherty, J. P. (1998). Regulated lentiviral packaging cell line devoid of most viral cis-acting sequences. Virology 249: 167-174.
5. Corbeau, P., Kraus, G., and Wong-Staal, F. (1996). Efficient gene transfer by a human immunodeficiency virus type 1 (HIV-1)-derived vector utilizing HIV packaging cell line. Proc. Natl. Acad. Sci. USA 93: 14070-14075.
6. Kafri, T., van Praag, H., Ouyang, L., Gage, F. H., and Verma, I. M. (1999). A packaging cell line for lentivirus vectors. J. Virol. 73: 576-584.
7. Zufferey, R., Nagy, D., Mandel, R. J., Naldini, L., and Trono, D. (1997). Multiply attenuated lentiviral vector achieves efficient gene delivery in vivo. Nat. Biotechnol. 15: 871-875.
8. Dull, T., et al. (1998). A third-generation lentivirus vector with a conditional packaging system. J. Virol. 72: 8463-8471.
9. Han, J. J., et al. (1999). Transgene expression in the guinea pig cochlea mediated by a lentivirus-derived gene transfer vector. Hum. Gene Ther. 10: 1867-1873.
10. Deglon, N., et al. (2000). Self-inactivating lentiviral vectors with enhanced transgene expression as potential gene transfer system in Parkinson's disease. Hum. Gene Ther. 11: 179-190.
11. Park, F., Ohashi, K., Chiu, W., Naldini, L., and Kay, M. A. (2000). Efficient lentiviral transduction of liver requires cell cycling in vivo. Nat. Genet. 24: 49-52.
12. Ory, D. S., Neugeboren, B. A., and Mulligan, R. C. (1996). A stable human-derived packaging cell line for production of high titer retrovirus/vesicular stomatitis virus G pseudotypes. Proc. Natl. Acad. Sci. USA 93: 11400-11406.
13. Zufferey, R., Donello, J. E., Trono, D., and Hope, T. J. (1999). Woodchuck hepatitis virus posttranscriptional regulatory element enhances expression of transgenes delivered by retroviral vectors. J. Virol. 73: 2886-2892.
14. Yang, Y., et al. (1995). Inducible, high-level production of infectious murine leukemia retroviral vector particle pseudotyped with vesicular stomatitis virus G envelope protein. Hum. Gene Ther. 6: 1203-1213.
15. Chen, S. T., Iida, A., Guo, L., Friedmann, T., and Yee, J. K. (1996). Generation of packaging cell lines for pseudotyped retroviral vectors of the G protein of vesicular stomatitis virus by using a modified tetracycline inducible system. Proc. Natl. Acad. Sci. USA93: 10057-10062.
16. Gossen, M., and Bujard, H. (1992). Tight control of gene expression in mammalian cells by tetracycline-responsive promoters. Proc. Natl. Acad. Sci. USA 89: 5547-5551.
17. Kaplan, A. H., and Swanstrom, R. (1991). The HIV-1 gag precursor is processed via two pathways: Implications for cytotoxicity. Biomed. Biochim. Acta 50: 647-653.