An N-terminal protein degradation tag enables robust selection of highly active enzymes

Biochemistry

Volumn 50, Issue 40, 2011, Pages 8594-8602

  Butz, Maren ^a   Neuenschwander, Martin ^a   Kast, Peter ^a   Hilvert, Donald ^a  

^a ETH ZURICH   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

CATALYTIC EFFICIENCIES; CHORISMATE; CHORISMATE MUTASE; DIRECTED EVOLUTION; FRAMESHIFT; N-TERMINALS; PEPTIDE SEQUENCES; PROTEIN CONCENTRATIONS; PROTEIN DEGRADATION; TARGET PROTEINS;

DEGRADATION; ENZYME ACTIVITY; EXPERIMENTS;

PROTEINS;

CHORISMATE MUTASE; PROTEIN TAG; PROTEIN TAG REPA; PROTEIN TAG SSRA; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; CATALYSIS; CONTROLLED STUDY; IN VITRO SELECTION; INTERMETHOD COMPARISON; INTRACELLULAR SPACE; MUTAGENESIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN CONTENT; PROTEIN DEGRADATION;

AMINO ACID MOTIFS; AMINO ACID SEQUENCE; ARCHAEAL PROTEINS; CATALYSIS; CHORISMATE MUTASE; CYCLOHEXANECARBOXYLIC ACIDS; CYCLOHEXENES; DIRECTED MOLECULAR EVOLUTION; KINETICS; METHANOCOCCALES; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PROTEIN ENGINEERING;

EID: 80053609136     PISSN: 00062960     EISSN: 15204995     Source Type: Journal    
DOI: 10.1021/bi2011338     Document Type: Article

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