Exploring the active site of chorismate mutase by combinatorial mutagenesis and selection: The importance of electrostatic catalysis

Proceedings of the National Academy of Sciences of the United States of America

Volumn 93, Issue 10, 1996, Pages 5043-5048

  Kast, Peter ^a   Asif Ullah, M ^a   Jiang, Ning ^a   Hilvert, Donald ^a  

^a SCRIPPS RESEARCH INSTITUTE   (United States)

Author keywords

aromatic amino acid biosynthesis; Bacillus subtilis; catalytic antibody; metabolic pathway engineering; random oligonucleotide library

Indexed keywords

AROMATIC AMINO ACID; CATALYTIC ANTIBODY; CHORISMATE MUTASE; OLIGONUCLEOTIDE;

AMINO ACID SYNTHESIS; ARTICLE; BACILLUS SUBTILIS; BIOSYNTHESIS; CATALYSIS; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME SUBSTRATE; ESCHERICHIA COLI; GENETIC ENGINEERING; GENETIC SELECTION; MUTAGENESIS; NONHUMAN; PRIORITY JOURNAL;

AMINO ACID SEQUENCE; BACILLUS SUBTILIS; BASE SEQUENCE; BINDING SITES; CATALYSIS; CHORISMATE MUTASE; DNA PRIMERS; ELECTROCHEMISTRY; ESCHERICHIA COLI; GENETIC COMPLEMENTATION TEST; GENETIC ENGINEERING; MOLECULAR SEQUENCE DATA; MOLECULAR STRUCTURE; MUTAGENESIS, SITE-DIRECTED; PHENOTYPE; SELECTION (GENETICS); VARIATION (GENETICS);

EID: 0029999832     PISSN: 00278424     EISSN: None     Source Type: Journal    
DOI: 10.1073/pnas.93.10.5043     Document Type: Article

References (47)

1. Haslam, E. (1993) Shikimic Acid: Metabolism and Metabolites (Wiley, New York).
2. Andrews, P. R., Cain, E. N., Rizzardo, E. & Smith, G. D. (1977) Biochemistry 16, 4848-4852.
3. Sogo, S. G., Widlanski, T. S., Hoare, J. H., Grimshaw, C. E., Berchtold, G. A. & Knowles, J. R. (1984) J. Am. Chem. Soc. 106, 2701-2703.
4. Copley, S. D. & Knowles, J. R. (1985) J. Am. Chem. Soc. 107, 5306-5308.
5. Lee, A. Y., Stewart, J. D., Clardy, J. & Ganem, B. (1995) Chem. Biol. 2, 195-203.
6. Chook, Y. M., Ke, H. & Lipscomb, W. N. (1993) Proc. Natl. Acad. Sci. USA 90, 8600-8603.
7. Chook, Y. M., Gray, J. V., Ke, H. & Lipscomb, W. N. (1994) J. Mol. Biol. 240, 476-500.
8. Lee, A. Y., Karplus, P. A., Ganem, B. & Clardy, J. (1995) J. Am. Chem. Soc. 117, 3627-3628.
9. Haynes, M. R., Stura, E. A., Hilvert, D. & Wilson, I. A. (1994) Science 263, 646-652.
10. Copley, S. D. & Knowles, J. R. (1987) J. Am. Chem. Soc. 109, 5008-5013.
11. Gajewski, J. J., Jurayj, J., Kimbrough, D. R., Gande, M. E., Ganem, B. & Carpenter, B. K. (1987) J. Am. Chem. Soc. 109, 1170-1186.
12. Addadi, L., Jaffe, E. K. & Knowles, J. R. (1983) Biochemistry 22, 4494-4501.
13. Kast, P., Asif-Ullah, M. & Hilvert, D. (1996) Tetrahedron Lett. 37, 2691-2694.
14. Sambrook, J., Fritsch, E. F. & Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual (Cold Spring Harbor Lab. Press, Plainview, NY), 2nd Ed.
15. Xia, T., Zhao, G., Fischer, R. S. & Jensen, R. A. (1992) J. Gen. Microbiol. 138, 1309-1316.
16. Zhao, G., Xia, T., Fischer, R. S. & Jensen, R. A. (1992) J. Biol. Chem. 267, 2487-2493.
17. Gray, J. V., Golinelli-Pimpaneau, B. & Knowles, J. R. (1990) Biochemistry 29, 376-383.
18. Short, J. M., Fernandez, J. M., Sorge, J. A. & Huse, W. D. (1988) Nucleic Acids Res. 16, 7583-7600.
19. Yanisch-Perron, C., Vieira, J. & Messing, J. (1985) Gene 33, 103-119.
20. Chang, A. C. Y. & Cohen, S. N. (1978) J. Bacteriol. 134, 1141-1156.
21. Kast, P. (1994) Gene 138, 109-114.
22. Sanger, F., Nicklen, S. & Coulson, A. R. (1977) Proc. Natl. Acad. Sci. USA 74, 5463-5467.
23. Maruya, A., O'Connor, M. J. & Backman, K. (1987) J. Bacteriol. 169, 4852-4853.
24. Chothia, C. (1984) Annu. Rev. Biochem. 53, 537-572.
25. Dube, D. K. & Loeb, L. A. (1989) Biochemistry 28, 5703-5707.
26. Evnin, L. B., Vásquez, J. R. & Craik, C. S. (1990) Proc. Natl. Acad. Sci. USA 87, 6659-6663.
27. Oliphant, A. R. & Struhl, K. (1989) Proc. Natl. Acad. Sci. USA 86, 9094-9098.
28. Reidhaar-Olson, J. F. & Sauer, R. T. (1988) Science 241, 53-57.
29. Liao, H., McKenzie, T. & Hageman, R. (1986) Proc. Natl. Acad. Sci. USA 83, 576-580.
30. Hermes, J. D., Blacklow, S. C. & Knowles, J. R. (1990) Proc. Natl. Acad. Sci. USA 87, 696-700.
31. Dorner, L. F. & Schildkraut, I. (1994) Nucleic Acids Res. 22, 1068-1074.
32. Munir, K. M., French, D. C., Dube, D. K. & Loeb, L. A. (1992) J. Biol. Chem. 267, 6584-6589.
33. Milla, M. E. & Sauer, R. T. (1995) Biochemistry 34, 3344-3351.
34. Dube, D. K., Black, M. E., Munir, K. M. & Loeb, L. A. (1993) Gene 137, 41-47.
35. Munir, K. M., French, D. C. & Loeb, L. A. (1993) Proc. Natl. Acad. Sci. USA 90, 4012-4016.
36. Blacklow, S. C. & Knowles, J. R. (1990) Biochemistry 29, 4099-4108.
37. Kim, S., Ribas de Pouplana, L. & Schimmel, P. (1993) Proc. Natl. Acad. Sci. USA 90, 10046-10050.
38. Gong, J. & Ferreira, G. C. (1995) Biochemistry 34, 1678-1685.
39. Wiest, O. & Houk, K. N. (1994) J. Org. Chem. 59, 7582-7584.
40. Kast, P., Hartgerink, J. D., Asif-Ullah, M. & Hilvert, D. (1996) J. Am. Chem. Soc. 118, 3069-3070.
41. Wiest, O. & Houk, K. N. (1995) J. Am. Chem. Soc. 117, 11628-11639.
42. Smith, P. J. & Wilcox, C. S. (1991) Tetrahedron 47, 2617-2628.
43. Lyne, P. D., Mulholland, A. J. & Richards, W. G. (1995) J. Am. Chem. Soc. 117, 11345-11350.
44. Gray, J. V., Eren, D. & Knowles, J. R. (1990) Biochemistry 29, 8872-8878.
45. Pourmotabbed, T., Dell'Acqua, M., Gerlt, J. A., Stanczyk, S. M. & Bolton, P. H. (1990) Biochemistry 29, 3677-3683.
46. Xue, Y. & Lipscomb, W. N. (1995) Proc. Natl. Acad. Sci. USA 92, 10595-10598.
47. Hilvert, D., Carpenter, S. H., Nared, K. D. & Auditor, M.-T. M. (1988) Proc. Natl. Acad. Sci. USA 85, 4953-4955.