Non-thermal effects of 2.45GHz microwaves on spindle assembly, mitotic cells and viability of Chinese hamster V-79 cells

Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis

Volumn 716, Issue 1-2, 2011, Pages 1-9

  Ballardin, Michela ^a   Tusa, Ignazia ^a   Fontana, Nunzia ^a   Monorchio, Agostino ^a   Pelletti, Chiara ^a   Rogovich, Alessandro ^a   Barale, Roberto ^a   Scarpato, Roberto ^a  

^a UNIVERSITY OF PISA   (Italy)

Author keywords

Apoptosis; Microwaves; Non thermal effects; Spindle aberration; V79 cells

Indexed keywords

ANIMAL CELL; APOPTOSIS; ARTICLE; CELL DAMAGE; CELL VIABILITY; CHINESE HAMSTER; CHINESE HAMSTER V 79 CELL; CONTROLLED STUDY; IMMUNOHISTOCHEMISTRY; MICROWAVE IRRADIATION; MITOSIS; MITOSIS INDEX; MITOSIS INHIBITION; MITOSIS SPINDLE; NONHUMAN; PRIORITY JOURNAL; RADIATION EXPOSURE; RADIOFREQUENCY; TEMPERATURE SENSITIVITY; THERMAL STIMULATION;

ANIMALS; APOPTOSIS; CELL DIVISION; CELL LINE; CELL SURVIVAL; CRICETINAE; CRICETULUS; HOT TEMPERATURE; MICROWAVES; MITOSIS; MITOTIC INDEX; MITOTIC SPINDLE APPARATUS;

CRICETULUS GRISEUS;

EID: 80053567771     PISSN: 00275107     EISSN: 09218262     Source Type: Journal    
DOI: 10.1016/j.mrfmmm.2011.07.009     Document Type: Article

References (66)

1. Research and Regulatory Efforts on Mobile Phone Health Issues, Report GAO-01-545 to Congressional Requesters, U.S. General Accounting Office, Washington, DC, 2001.
2. Vijayalaxmi, Obe G. Controversial cytogenetic observations in mammalian somatic cells exposed to radiofrequency radiation. Radiat. Res. 2004, 162:481-496.
3. Vijayalaxmi, Obe G. Controversial cytogenetic observations in mammalian somatic cells exposed to extremely low frequency electromagnetic radiation: a review and future research recommendations. Bioelectromagnetics 2005, 26:412-430.
4. Röösli M. Radiofrequency electromagnetic field exposure and non-specific symptoms of ill health: a systematic review. Environ. Res. 2008, 107:277-287.
5. Hardell L., Sage C. Biological effects from electromagnetic field exposure and public exposure standards. Biomed. Pharmacother. 2008, 62:104-109.
6. Ruediger H.W. Genotoxic effects of radiofrequency electromagnetic fields. Pathophysiology 2009, 16:89-102.
7. Brusick D., Albertini R., McRee D., Peterson D., Williams G., Hanawalt P., Preston J. Genotoxicity of radiofrequency radiation. Environ. Mol. Mutagen. 1998, 32:1-16.
8. Meltz M.L. Radiofrequency exposure and mammalian cell toxicity genotoxicity, and transformation. Bioelectromagnectics 2003, (Suppl. 6):S196-S213.
9. Verschaeve L., Maes A. Genetic, carcinogenic and teratogenic effects of radiofrequency fields. Mutat. Res. 1998, 410:141-165.
10. Verschaeve L. Genetic effects of radiofrequency radiation (RFR). Toxicol. Appl. Pharmacol. 2005, 207:336-341.
11. Maes A., Collier M., Slaets D., Verschaeve L. 954MHz microwaves enhance the mutagenic properties of mitomycin C. Environ. Mol. Mutagen. 1996, 28:26-30.
12. Zhang M.B., He J.L., Jin L.F., Lu D.Q. Study of low-intensity 2450-MHz microwave exposure enhancing the genotoxic effects of mitomycin C using micronucleus test and comet assay in vitro. Biomed. Environ. Sci. 2002, 15:283-290.
13. Adair R.K. Biophysical limits on athermal effects of RF and microwave radiation. Bioelectromagnetics 2003, 24:39-48.
14. Sheppard A.R., Swicord M.L., Balzano Q. Quantitative evaluations of mechanisms of radiofrequency interactions with biological molecules and processes. Health Phys. 2008, 95:365-395.
15. Cifra M., Pokorný J., Havelka D., Kučera O. Electric field generated by axial longitudinal vibration modes of microtubule. BioSystems 2010, 100:122-131.
16. Porcelli M., Cacciapuoti G., Fusco S., Massa R., D'Ambrosio G., Bertoldo C., De Rosa M., Zappia V. Non-thermal effects of microwaves on proteins: thermophylic enzymes as model system. FEBS Lett. 1997, 402:102-106.
17. Fröhlich H. The biological effects of microwaves and related questions. Advances in Electronics and Electron Physics 1980, 53:85-152. Elsevier Inc., Department of Physics University of Liverpool, Liverpool, England.
18. Marani E., Feirabend H.K.P. Future perspectives in microwave applications in life sciences. Eur. J. Morphol. 1994, 32:330-334.
19. Kirschvink J.L. Microwave absorption by magnetite: a possible mechanism for coupling nonthermal levels of radiation to biological systems. Bioelectromagnetics 1996, 17:187-194.
20. Taylor L.S. The mechanisms of athermal microwave biological effects. Bioelectromagnetics 1981, 2:259-267.
21. Fesenko E.E., Geletyuk V.I., Kazachenko V.N., Chemeris N.K. Preliminary microwave irradiation of water solutions changes their channel-modifying activity. FEBS Lett. 1995, 366:49-52.
22. Sciandrello G., Caradonna F., Barbata G. Karyotype abnormalities in a variant Chinese hamster cell line resistant to methyl methanesulphonate. Hereditas 1996, 24:39-46.
23. Schiebel E. Tubulin complexes: binding to the centrosome, regulatin and microtubule nucleation. Curr. Opin. Cell Biol. 2000, 12:113-118.
24. Kirkland D.J. Statistical Evaluation of Mutagenicity Test Data 1989, Cambridge University Press, Cambridge, UK.
25. Dardano A., Ballardin M., Ferdeghini M., Lazzeri E., Traino C., Caraccio N., Mariani G., Barale R., Monzani F. Anticlastogenic effect of Ginkgo biloba extract in Grave's disease patients receiving radioiodine therapy. J. Clin. Endocrinol. Metab. 2007, 92:4286-4292.
26. Fitzsimmons R.J., Ryaby J.T., Mohan S., Magee F.P., Baylink D.J. Combined magnetic fields increase insulin-like growth factor-II in TE-85 human osteosarcoma bone cell cultures. Endocrinology 1995, 136:3100-3106.
27. Belyaev I.Y., Eriksson S., Nygren J., Torudd J., Harms-Ringdahl M. Effects of ethidium bromide on DNA loop organisation in human lymphocytes measured by anomalous viscosity time dependence and single cell gel electrophoresis. Biochim. Biophys. Acta 1999, 1428:348-356.
28. Blackman C.F., Benane S.G., Rabinowitz J.R., House D.E., Joines W.T. A role for the magnetic field in the radiation-induced efflux of calcium ions from brain tissue in vitro. Bioelectromagnetics 1985, 6:327-337.
29. Smith S.D., McLeod B.R., Liboff A.R. Calcium cyclotron resonance and diatom mobility. Bioelectromagnetics 1987, 8:215-227.
30. Prato F.S., Carson J.J., Ossenkopp K.P., Kavaliers M. Possible mechanisms by which extremely low frequency magnetic fields affect opioid function. FASEB J. 1995, 9:807-814.
31. Alipov Y.D., Belyaev I.Y., Aizenberg O.A. Systemic reaction of E. coli cells to weak electromagnetic fields of extremely low frequency. Bioelectrochem. Bioenerg. 1994, 34:5-12.
32. Alipov Y.D., Belyaev I.Y. Difference in frequency spectrum of ELF effect on the genome conformational state of AB1157 and EMG2 E. coli cells. Bioelectromagnetics 1996, 17:384-387.
33. Belyaev I.Y., Alipov Y.D., Matronchik A.Y., Radko S.P. Cooperativity in E. coli cell response to resonance effect of weak extremely low frequency electromagnetic field. Bioelectrochem. Bioenerg. 1995, 37:85-90.
34. Blackman C.F., House D.E., Benane S.G., Joines W.T., Spiegel R.J. Effects of ambient levels of power-line-frequency electric fields on a developing vertebrate. Bioelectromagnetics 1988, 9:129-140.
35. Goodman E.M., Greenebaum B., Marron M.T. Effects of electromagnetic fields on molecules and cells. Int. Rev. Cytol. 1995, 158:279-338.
36. Lin H., Goodman R., Shirley-Henderson A. Specific region of the c-myc promoter is responsive to electric and magnetic fields. J. Cell. Biochem. 1994, 54:281-288.
37. Phillips J.L., Haggren W., Thomas W.J., Ishida-Jones T., Adey W.R. Magnetic field-induced changes in specific gene transcription. Biochim. Biophys. Acta 1992, 1132:140-144.
38. Lindstrom E., Berglund A., Mild K.H., Lindstrom P., Lundgren E. CD45 phosphatase in Jurkat cells is necessary for response to applied ELF magnetic fields. FEBS Lett. 1995, 370:118-122.
39. Niehaus M., Bruggemeyer H., Behre H.M., Lerchl A. Growth retardation, testicular stimulation, and increased melatonin synthesis by weak magnetic fields (50Hz) in Djungarian hamsters, Phodopus sungorus. Biochem. Biophys. Res. Commun. 1997, 234:707-711.
40. Roy S., Noda Y., Eckert V., Traber M.G., Mori A., Liburdy R., Packer L. The phorbol 12-myristate 13-acetate (PMA)-induced oxidative burst in rat peritoneal neutrophils is increased by a 0.1mT (60Hz) magnetic field. FEBS Lett. 1995, 376:164-166.
41. Uckun F.M., Kurosaki T., Jin J., Jun X., Morgan A., Takata M., Bolen J., Luben R. Exposure of B-lineage lymphoid cells to low energy electromagnetic fields stimulates Lyn kinase. J. Biol. Chem. 1995, 270:27666-27670.
42. McCann J., Dietrich F., Rafferty C. The genotoxic potential of electric and magnetic fields: an update. Mutat. Res. 1998, 411:45-86.
43. Tofani S., Ferrara A., Anglesio L., Gilli G. Evidence of genotoxic effects of resonant ELF magnetic fields. Bioelectrochem. Bioenerg. 1995, 36:9-13.
44. Schrader T., Munter K., Kleine-Ostmann T., Schmid E. Spindle disturbances in human-hamster hybrid (AL) cells induced by mobile communication frequency range signals. Bioelectromagnetics 2008, 29:626-639.
45. Compton D.A. Spindle assembly in animal cells. Annu. Rev. Biochem. 2000, 69:95-114.
46. Inoue S., Salmon E.D. Force generation by microtubule assembly/disassembly in mitosis and related movements. Mol. Biol. Cell 1995, 6:1619-1640.
47. Duesberg P., Slindl R., Hehlmann R. Explaining the high mutation rates of cancer cells in drug and multidrug resistance by chromosome reassortment that are catalysed by aneuploidy. Proc. Natl. Acad. Sci. U. S. A. 2000, 97:14295-14300.
48. Heald R., Tournebize R., Habermann A., Karsenti E., Hyman A. Spindle assembly in Xenopus egg extracts: respective roles of centrosomes and microtubule self-organization. J. Cell Biol. 1997, 138:615-628.
49. Khodjakov A., Rieder C. Centrosomes enhance the fidelity of cytokinesis in vertebrates and are required for cell cycle progression. J. Cell Biol. 2001, 153:237-242.
50. Pluta A.F., Mackay A.M., Ainsztein A.M., Goldberg I.G., Earnshaw W.C. The centromere: hub of chromosomal activities. Science 1995, 270:1591-1594.
51. Pihan G.A., Purohit A., Wallace J., Knecht H., Woda B., Quesenberry P., Doxsey S.J. Centrosome defects and genetic instability in malignant tumours. Cancer Res. 1998, 58:3974-3985.
52. Brinkley B.R. Managing the centrosome numbers game: from chaos to stability in cancer cell division. Trends Cell Biol. 2001, 11:18-21.
53. Ortner M.J., Galvin M.J., Irwin R.D. The effect of 2450-MHz microwave radiation during microtubular polymerization in vitro. Radiat. Res. 1983, 93:353-363.
54. Kerr J.F.R., Wyllie A.H., Currie A.R. Apoptosis: a basic biological phenomenon with wide ranging implications in tissue kinetics. Br. J. Cancer 1972, 26:239-257.
55. Nicholson D.W., Thornberry N.A. Caspases: killer proteases. Trends Biochem. Sci. 1997, 22:299-306.
56. Mathiasen I.S., Jaattela M. Triggering caspase-independent cell death to combat cancer. Trends Mol. Med. 2002, 8:212-220.
57. Capri M., Scarcella E., Bianchi E., Fumelli C., Mesirca P., Agostini P., Remondini D., Schuderer J., Kuster N., Bersani F. 1800MHz radiofrequency (mobile phones, different Global System for Mobile communication modulations) does not affect apoptosis and heat shock protein 70 level in peripheral blood mononuclear cells from young and old donors. Int. J. Radiat. Biol. 2004, 80:389-397.
58. Capri M., Scarcella E., Fumelli C., Bianchi E., Salvioli S., Mesirca P., Agostini C., Antolini A., Schiavoni A., Franceschi C. In vitro exposure of human lymphocytes to 900MHz CW and GSM modulated radiofrequency: studies of proliferation, apoptosis and mitochondrial membrane potential. Radiat. Res 2004, 162:211-218.
59. Hook G.J., Zhang P., Lagroye I., Li L., Higashikubo R., Moros E.G., Straube W.L., Pickard W.J., Baty J.D., Roti J.L. Measurement of DNA damage and apoptosis in Molt-4 cells after in vitro exposure to radiofrequency radiation. Radiat. Res. 2004, 161:193-200.
60. Maeda K., Maeda T., Qi Y. In vitro and in vivo induction of human LoVo cells into apoptotic process by non-invasive microwave treatment: a potentially novel approach for physical therapy of human colorectal cancer. Oncol. Rep. 2004, 11:771-775.
61. Caraglia M., Marra M., Mancinelli F., D'Ambrosio G., Massa R., Giordano A., Budillon A., Abruzzese A., Bismuto E. Electromagnetic fields at mobile phone frequency induce apoptosis and inactivation of the multi-chaperone complex in human epidermoid cancer cells. J. Cell. Physiol. 2005, 204:539-548.
62. Marinelli F., La Sala D., Cicciotti G., Cattini L., Trimarchi C., Putti S., Zamparelli A., Giuliani L., Tomassetti G., Cinti C. Exposure to 900MHz electromagnetic field induces an unbalance between pro-apoptotic and pro-survival signals in T-lymphoblastoid leukemia CCRF-CEM cells. J. Cell. Physiol. 2004, 198:324-332.
63. Rosefort C., Fauth E., Zankl H. Micronuclei induced by aneugens and clastogens in mononucleate and binucleate cells using the cytokinesis block assay. Mutagenesis 2004, 19:277-284.
64. GTEM test cells - test cells for EMC radiated & immunity testing DC to 20GHz SCHAFFNER, pp. 2-5.
65. Fontana N., Pelletti C., Rogovich A., Monorchio A. On the influence of a glass slide on the SAR distribution in Petri dishes for in vitro exposure to 2.45GHz EM fields. IEEE International APS and USNC/URSI National Radio Science Meeting 2010.
66. Kuster N., Schonborn F. Recommended minimal requirements and development guidelines for exposure setups of bio-experiments addressing the health risk concern of wireless communications. Bioelectromagnetics 2000, 21:508-514.