Comparison of DNA extraction methods and development of duplex PCR and real-time PCR to detect tomato, carrot, and celery in food

Journal of Agricultural and Food Chemistry

Volumn 59, Issue 19, 2011, Pages 10414-10424

  Pafundo, Simona ^a   Gullì, Mariolina ^a   Marmiroli, Nelson ^a  

^a UNIVERSITY OF PARMA   (Italy)

Author keywords

allergens; carrot; celery; duplex PCR; real time PCR; SYBR Green; tomato

Indexed keywords

ALLERGENS; CARROT; CELERY; DUPLEX PCR; REAL-TIME PCR; SYBR GREEN; TOMATO;

ANTIGENS; DNA; FRUITS; VEGETABLES;

POLYMERASE CHAIN REACTION;

ALLERGEN; PLANT DNA;

ARTICLE; CARROT; CELERY; COMPARATIVE STUDY; FOOD ALLERGY; FOOD ANALYSIS; GENETICS; IMMUNOLOGY; METHODOLOGY; POLYMERASE CHAIN REACTION; TOMATO;

ALLERGENS; APIUM GRAVEOLENS; DAUCUS CAROTA; DNA, PLANT; FOOD ANALYSIS; FOOD HYPERSENSITIVITY; LYCOPERSICON ESCULENTUM; POLYMERASE CHAIN REACTION;

APIUM GRAVEOLENS VAR. DULCE; DAUCUS CAROTA; LYCOPERSICON ESCULENTUM;

EID: 80053539096     PISSN: 00218561     EISSN: 15205118     Source Type: Journal    
DOI: 10.1021/jf202382s     Document Type: Article

References (36)

1. Vieths, S.; Lüttkopf, D.; Reindl, J.; Anliker, M. D.; Wüthrich, B.; Ballmer-Weber, B. K. Allergens in celery and zucchini Allergy 2002, 57, 100-105 (Pubitemid 34970048)
2. Hernández, M.; Esteve, T.; Pla, M. Real-time polymerase chain reaction based assays for quantitative detection of barley, sunflower, and wheat J. Agric. Food Chem. 2005, 53, 7003-7009 (Pubitemid 41375306)
3. Carcea, M.; Brereton, P.; Hsu, R.; Kelly, S.; Marmiroli, N.; Melini, F.; Soukoulis, C.; Wenping, D. Food authenticity assessment: ensuring compliance with food legislation and traceability requirements. Quality Assur Saf. Crops Foods 2009, 1, 93-100
4. Commission Directive 2000/13/EC. Off. J. Eur. Union 2000, L109, 29-42
5. Commission Directive 2003/89/EC. Off. J. Eur. Union 2003, L308, 15-18.
6. Commission Directive 2006/142/EC. Off. J. Eur. Union 2006, L368, 110-111.
7. Commission Directive 2007/68/EC. Off. J. Eur. Union 2007, L 310, 11-14
8. Holzhauser, T.; Stephan, O.; Vieths, S. Detection of potentially allergenic hazelnut (Corylus avellana) residues in food: a comparative study with DNA PCR-ELISA and protein sandwich-ELISA J. Agric. Food Chem. 2002, 50, 5808-5815 (Pubitemid 35155298)
9. van Hengel, A. J. Food allergen detection methods and the challenge to protect food-allergic consumers Anal. Bioanal. Chem. 2007, 389, 111-118 (Pubitemid 47325217)
10. Di Pinto, A.; Forte, V. T.; Guastadisegni, M. C.; Martino, C.; Schena, F. P.; Tantillo, G. A comparison of DNA extraction methods for food analysis Food Control 2007, 18, 76-80 (Pubitemid 43964474)
11. Pafundo, S.; Gullì, M.; Marmiroli, N. SYBR GreenER real-time PCR to detect almond in traces in processed food Food Chem. 2009, 116, 811-815
12. Poms, R. E.; Klein, C. L.; Anklam, E. Methods for allergen analysis in food: a review Food Addit. Contam. 2004, 21, 1-31 (Pubitemid 38208313)
13. Pafundo, S.; Gullì, M.; Marmiroli, N. Multiplex real-time PCR using SYBR GreenER for the detection of DNA allergens in food Anal. Bioanal. Chem. 2010, 396, 1831-1839
14. Yeung, J. Enzyme-linked immunosorbent assays (ELISAs) for detecting allergens in foods. In Detecting Allergens in Food; Koppelman, S. J.; Hefle, S. L., Eds.; CRC Press: Boca Raton, FL, 2006; pp 109-124.
15. Holzhauser, T.; Stephan, O.; Vieths, S. Polymerase chain reaction (PCR) methods for the detection of allergenic foods. In Detecting Allergens in Food; Koppelman, S. J.; Hefle, S. L., Eds.; CRC Press: Boca Raton, FL, 2006; pp 125-143.
16. Marmiroli, N.; Maestri, E. Polymerase chain reaction (PCR). In Food Toxicants Analysis. Techniques, Strategies and Development; Picò, Y., Ed.; Elsevier: Amsterdam, The Netherlands, 2007; pp 147-187.
17. Kirsch, S.; Fourdrilis, S.; Dobson, R.; Scippo, M.-L.; Maughuin-Rogister, G.; De Pauw, E. Quantitative methods for food allergens: a review Anal. Bioanal. Chem. 2009, 395, 57-67
18. Stack, S. M.; Royer, S. M.; Shearer, L. A.; Chang, S. B.; Giovannoni, J. J.; Westfall, D. H.; White, R. A.; Anderson, L. K. Role of fluorescence in situ hybridization in sequencing the tomato genome Cytogenet. Genome Res. 2009, 124, 339-350
19. Petersen, A.; Vieths, S.; Aulepp, H.; Schlaak, M.; Becker, W. M. Ubiquitous structures responsible for IgE cross reactivity between tomato fruit and grass pollen allergens J. Allergy Clin. Immunol. 1996, 98, 805-815 (Pubitemid 26357248)
20. Crespo, J. F.; Rodriguez, J. Food allergy in adulthood Allergy 2003, 58, 98-113 (Pubitemid 36359829)
21. Yang, X.; Quiros, C. F. Characterizing the celery genome with DNA-based genetic markers J. Am. Soc. Hortic. Sci. 1995, 120, 747-751
22. Yang, X.; Quiros, C. F. Construction of a genetic linkage map in celery using DNA-based markers Genome 1995, 38, 36-44
23. Hupfer, C.; Waiblinger, H.-U.; Bush, U. Development and validation of a real-time PCR detection method for celery in food Eur. Food Res. Technol. 2007, 225, 329-335 (Pubitemid 46945649)
24. Ballmer-Weber, B. K.; Wüthrich, B.; Wangorsch, A.; FÖtisch, K.; Altmann, F.; Vieths, S. Carrot allergy: double-blinded, placebo controlled food challenge and identification of allergens J. Allergy Clin. Immunol. 2001, 108, 301-307 (Pubitemid 32735419)
25. Bennett, M. D.; Smith, J. B. Nuclear DNA amount in angiosperms Philos. Trans. R. Soc. London B: Biol. Sci. 1991, B334, 309-345
26. Mustorp, S.; Engahl-Axelsson, C.; Svensson, U.; Holck, A. Detection of celery (Apium graveolens), mustard (Sinapis alba, Brassica juncea, Brassica nigra) and sesame (Sesamum indicum) in food by real-time PCR Eur. Food Res. Technol. 2008, 226, 771-778
27. Dovičovičová, L.; Olexová, L.; Pangallo, D.; Siekel, P.; Kuchta, T. Polymerase chain reaction (PCR) for the detection of celery (Apium graveolens) in food Eur. Food Res. Technol. 2004, 218, 493-495 (Pubitemid 40871441)
28. Chaouachi, M.; El Malki, R.; Berard, A.; Romaniuk, M.; Laval, V.; Brunel, D.; Bertheau, Y. Development of a real-time PCR method for the differential detection and quantification of four solanaceae in GMO analysis: potato (Solanum tuberosum), tomato (Solanum lycopersicum), eggplant (Solanum melongena), and pepper (Capsicum annuum) J. Agric. Food Chem. 2008, 56, 1818-1828 (Pubitemid 351497835)
29. Turci, M.; Savo Sardaro, M. L.; Visioli, G.; Maestri, E.; Marmiroli, M.; Marmiroli, N. Evaluation of DNA extraction procedures from traceability of various tomato products Food Control 2010, 21, 143-149
30. Zagon, J.; Jansen, B.; Knoppik, M.; Ehlers, A.; Kroh, L. W.; Holzauser, T.; Vieths, S.; Broll, H. Gene transcription analysis of carrot allergens by relative quantification with single and duplex reverse transcription real-time PCR Anal. Bioanal. Chem. 2010, 396, 483-493
31. Doyle, J. J.; Doyle, J. L. Isolation of plant DNA from fresh tissue Am. J. Bot. 1988, 75, 1238
32. Meyer, R.; Chardonnens, F.; Hübner, P.; Lüthy, J. Polymerase chain reaction (PCR) in the quality and safety assurance of food: detection of soya in processed meat products Z. Lebensm. Unters. Forsch. A 1996, 203, 339-344
33. Vickers, A. J. Parametric versus non-parametric statistics in the analysis of randomized trials with non-normally distributed data BMC Med. Res. Methodol. 2005, 5, 35-46
34. Cankar, K.; štebih, D.; Dreo, T.; žel, J.; Gruden, K. Critical points of DNA quantification by real-time PCR - effects of DNA extraction method and sample matrix on quantification of genetically modified organisms BMC Biotechnol. 2006, 6, 37
35. Pafundo, S.; Agrimonti, C.; Marmiroli, N. Traceability of plant contribute in olive oil by AFLPs J. Agric. Food Chem. 2005, 53, 6995-7002
36. Zhu, L.-J.; Altmann, S. W. mRNA and 18S-RNA coapplication-reverse transcription for quantitative gene expression analysis Anal. Biochem. 2005, 345, 102-109 (Pubitemid 41558200)