Whole-cell imaging at nanometer resolutions using fast and slow focused helium ions

Biophysical Journal

Volumn 101, Issue 7, 2011, Pages 1788-1793

  Chen, Xiao ^a   Udalagama, Chammika N B ^a   Chen, Ce Belle ^a   Bettiol, Andrew A ^a   Pickard, Daniel S ^a   Venkatesan, T ^a   Watt, Frank ^a  

^a NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

Author keywords

[No Author keywords available]

Indexed keywords

HELIUM;

ARTICLE; CHEMISTRY; CYTOLOGY; ECHOGRAPHY; ELECTRON MICROSCOPY; HUMAN; LIVER; METHODOLOGY; MOLECULAR IMAGING; MONTE CARLO METHOD; NANOTECHNOLOGY; TIME;

HELIUM; HUMANS; LIVER; MICROSCOPY, ELECTRON; MOLECULAR IMAGING; MONTE CARLO METHOD; NANOTECHNOLOGY; TIME FACTORS;

EID: 80053374370     PISSN: 00063495     EISSN: 15420086     Source Type: Journal    
DOI: 10.1016/j.bpj.2011.08.028     Document Type: Article

References (24)

1. E. Betzig, and G.H. Patterson H.F. Hess Imaging intracellular fluorescent proteins at nanometer resolution Science 313 2006 1642 1645 (Pubitemid 44414039)
2. S.T. Hess, and T.J. Gould J. Zimmerberg Dynamic clustered distribution of hemagglutinin resolved at 40 nm in living cell membranes discriminates between raft theories Proc. Natl. Acad. Sci. USA 104 2007 17370 17375 (Pubitemid 350219826)
3. B. Huang, and W. Wang X. Zhuang Three-dimensional super-resolution imaging by stochastic optical reconstruction microscopy Science 319 2008 810 813 (Pubitemid 351225870)
4. B. Huang, and S.A. Jones X. Zhuang Whole-cell 3D STORM reveals interactions between cellular structures with nanometer-scale resolution Nat. Methods 5 2008 1047 1052
5. K.I. Willig, and S.O. Rizzoli S.W. Hell STED microscopy reveals that synaptotagmin remains clustered after synaptic vesicle exocytosis Nature 440 2006 935 939
6. L. Schermelleh, and P.M. Carlton J.W. Sedat Subdiffraction multicolor imaging of the nuclear periphery with 3D structured illumination microscopy Science 320 2008 1332 1336 (Pubitemid 351929468)
7. S.A. Müller, U. Aebi, and A. Engel What transmission electron microscopes can visualize now and in the future J. Struct. Biol. 163 2008 235 245
8. O. Medalia, and I. Weber W. Baumeister Macromolecular architecture in eukaryotic cells visualized by cryoelectron tomography Science 298 2002 1209 1213 (Pubitemid 35285482)
9. V. Lucić, F. Förster, and W. Baumeister Structural studies by electron tomography: from cells to molecules Annu. Rev. Biochem. 74 2005 833 865 (Pubitemid 40995525)
10. A. Leis, and B. Rockel W. Baumeister Visualizing cells at the nanoscale Trends Biochem. Sci. 34 2009 60 70
11. W. Chao, and B.D. Harteneck D.T. Attwood Soft X-ray microscopy at a spatial resolution better than 15 nm Nature 435 2005 1210 1213 (Pubitemid 40943082)
12. X. Huang, and J. Nelson C. Jacobsen Soft X-ray diffraction microscopy of a frozen hydrated yeast cell Phys. Rev. Lett. 103 2009 198101
13. H. Jiang, and C. Song J. Miao Quantitative 3D imaging of whole, unstained cells by using X-ray diffraction microscopy Proc. Natl. Acad. Sci. USA 107 2010 11234 11239
14. Y. Nishino, and Y. Takahashi K. Maeshima Three-dimensional visualization of a human chromosome using coherent X-ray diffraction Phys. Rev. Lett. 102 2009 018101
15. T.M.C. Tan, K.H. Sit, and K.P. Wong Kinetics of sulphate conjugation in extracts of human foetal liver cells in culture Biochem. Pharmacol. 37 1988 4629 4633 (Pubitemid 19006301)
16. R. Minqin, and J.A. van Kan F. Watt Nano-imaging of single cells using STIM Nucl. Instrum. Methods Phys. Res. B 260 2007 124 129 (Pubitemid 46874648)
17. A.A. Bettiol, C. Udalagama, and F. Watt A new data acquisition and imaging system for nuclear microscopy based on a field programmable gate array card Nucl. Instrum. Methods Phys. Res. B 267 2009 2069 2072
18. M.T. Postek, and A.E. Vladar S. McVey Helium ion microscopy: a new technique for semiconductor metrology and nanotechnology Proc. Aip Conf. 931 2007 161 167
19. L. Scipioni, and L.A. Stern B. Griffin Helium ion microscope Adv. Mater. Process 166 2008 27 30
20. D. Cohen-Tanugi, and N. Yao Superior imaging resolution in scanning helium-ion microscopy: a look at beam-sample interactions J. Appl. Phys. 104 2008 063504
21. F. Watt, and X. Chen A. Bettiol The Singapore high resolution single cell imaging facility Nucl. Instrum. Methods Phys. Res. B 2011 In press
22. Biersack, J. P. The Monte Carle program TRIM. http://www.srim.org.
23. NIST. http://physics.nist.gov/PhysRefData/Star/Text/ASTAR.html.
24. C. Udalagama, A.A. Bettiol, and F. Watt Stochastic spatial energy deposition profiles for MeV protons and keV electrons Phys. Rev. B 80 2009 224107