Macromolecular architecture in eukaryotic cells visualized by cryoelectron tomography

Science

Volumn 298, Issue 5596, 2002, Pages 1209-1213

  Medalia, Ohad ^a   Weber, Igor ^a   Frangakis, Achilleas S ^a   Nicastro, Daniela ^a   Gerisch, Günther ^a   Baumeister, Wolfgang ^a  

^a MAX PLANCK INSTITUTE OF BIOCHEMISTRY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

BIOLOGICAL MEMBRANES; CELLS; MACROMOLECULES; PROTEINS; TOMOGRAPHY;

ACTIN CYTOSKELETON;

SUPRAMOLECULAR CHEMISTRY;

PROTEASOME; PROTEIN;

MICROBIOLOGY;

ACTIN FILAMENT; ARTICLE; CROSS LINKING; CRYOELECTRON MICROSCOPY; CYTOPLASM; DICTYOSTELIUM; EUKARYOTIC CELL; MACROMOLECULE; MICROFILAMENT; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; THREE DIMENSIONAL IMAGING;

ACTINS; ANIMALS; BINDING SITES; CELL MEMBRANE; CELL MOVEMENT; DICTYOSTELIUM; ENDOPLASMIC RETICULUM, ROUGH; FREEZING; IMAGE PROCESSING, COMPUTER-ASSISTED; MACROMOLECULAR SUBSTANCES; MICROFILAMENT PROTEINS; MICROFILAMENTS; ORGANELLES; PEPTIDE HYDROLASES; PROTEASOME ENDOPEPTIDASE COMPLEX; PROTEOME; PROTOZOAN PROTEINS; RIBOSOMES; TOMOGRAPHY;

DICTYOSTELIUM; EUKARYOTA; PROTOZOA;

EID: 0037044862     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.1076184     Document Type: Article

References (45)

1. W. Baumeister, R. Grimm, J. Walz, Trends Cell Biol. 9, 81 (1999).
2. B. F. McEwen, J. Frank, Curr. Opin. Neurobiol. 11, 594 (2001).
3. R. G. Hart, Science 159, 1464 (1968).
4. A. J. Koster et al., J. Struct. Biol. 120, 276 (1997).
5. Reviewed in B. F. McEwen, M. Marko, J. Histochem. Cytochem. 49, 553 (2001).
6. K. Dierksen, D. Typke, R. Hegerl, A. J. Koster, W. Baumeister, Ultramicroscopy 40, 71 (1992).
7. K. Dierksen, D. Typke, R. Hegerl, W. Baumeister, Ultramicroscopy 49, 109 (1993).
8. R. Hegerl., W. Hoppe, Z. Naturforsch. Sect. A Phys. Sci. 31, 1717 (1976).
9. B. F. McEwen, K. H. Downing, R. M. Glaeser, Ultramicroscopy 60, 357 (1995).
10. R. Grimm et al., Biophys. J. 72, 482 (1997).
11. R. Grimm et al., Biophys. J. 74, 1031 (1998).
12. D. Nicastro, A. S. Frangakis, D. Typke, W. Baumeister, J. Struct. Biol. 129, 48 (2000).
13. R. Grimm, A. J. Koster, U. Ziese, D. Typke, W. Baumeister, J. Microsc. 183, 60 (1996).
14. Materials and methods are available as supporting material on Science Online.
15. A. Muller-Taubenberger et al., EMBO J. 20, 6772 (2001).
16. D. Voges, P. Zwickl, W. Baumeister, Annu. Rev. Biochem. 68, 1015 (1999).
17. J. M. Peters, Z. Cejka, J. R. Harris, J. A. Kleinschmidt, W. Baumeister, J. Mol. Biol. 234, 932 (1993).
18. M. Rechsteiner, L. Hoffman, W. Dubiel, J. Biol. Chem. 268, 6065 (1993).
19. H. Holzl et al., J. Cell Biol. 150, 119 (2000).
20. M. H. Glickman et al., Cell 94, 615 (1998).
21. J. E. Heuser, M. W. Kirschner, J. Cell Biol. 86, 212 (1980).
22. T. M. Svitkina, A. B. Verkhovsky, K. M. McQuade, G. G. Borisy, J. Cell Biol. 139, 397 (1997).
23. L. A. Flanagan et al., J. Cell Biol. 155, 511 (2001).
24. G. P. Resch, K. N. Goldie, A. Krebs, A. Hoenger, J. V. Small., J. Cell Sci. 115, 1877 (2002).
25. Reviewed in J. E. Bear, M. Krause, F. B. Gertler, Curr. Opin. Cell Biol. 13, 158 (2001).
26. Reviewed in D. Pantaloni, C. Le Clainche, M. F. Carlier, Science 292, 1502 (2001).
27. Reviewed in J. V. Small, T. Stradal, E. Vignal, K. Rottner, Trends Cell Biol. 12, 112 (2002).
28. C. Hug et al., Cell 81, 591 (1995).
29. M. Haugwitz, A. A. Noegel, J. Karakesisoglou, M. Schleicher, Cell 79, 303 (1994).
30. J. Niewöhner, I. Weber, M. Maniak, A. Muller-Taubenberger, G. Gerisch, J. Cell Biol. 138, 349 (1997).
31. M. Tsujioka, L. M. Machesky, S. L. Cole, K. Yahata, K. Inouye, Curr. Biol. 9, 389 (1999).
32. E. L. de Hostos, Trends Cell Biol. 9, 345 (1999).
33. R. Insall et al., Cell Motil. Cytoskel. 50, 115 (2001).
34. T. P. Stossel et al., Nature Rev. Mol. Cell. Biol. 2, 138 (2001).
35. E. Lee, K. Pang, D. A. Knecht, Biochim. Biophys. Acta 1525, 217 (2001).
36. J. L. Podoski, T. L. Steck, J. Biol. Chem. 265, 1312 (1990).
37. R. D. Mullins, J. A. Heuser, T. D. Pollard, Proc. Natl. Acad. Sci. U.S.A. 95, 6181 (1998).
38. H. N. Higgs, T. D. Pollard, Annu. Rev. Biochem. 70, 649 (2001).
39. M. Schliwa, Nature Rev. Mol. Cell. Biol. 3, 291 (2002).
40. Reviewed in R. Grünewald, O. Medalia, A. Gross, A. C. Steven, W. Baumeister, Biophys. Chem., in press.
41. J. Böhm et al., Proc. Natl. Acad. Sci. U.S.A. 97, 14245 (2000).
42. A. S. Frangakis et al., Proc. Natl. Acad. Sci. U.S.A. 99, 14153 (2002).
43. J. M. Plitzko et al., Trends Biotechnol. 20, S40 (2002).
44. J. Walz et al., J. Struct. Biol. 121, 19 (1998).
45. O. M. thanks the European Commission for a Marie Curie fellowship. I.W. was supported by a grant from the Deutsche Forschungsgemeinschaft (SFB 266 to G.G.). We thank J. Köhler for the movie and A. C. Steven for critical reading of the manuscript.