Application of imaging capillary IEF for characterization and quantitative analysis of recombinant protein charge heterogeneity

Electrophoresis

Volumn 29, Issue 21, 2008, Pages 4368-4376

  Sosic, Zoran ^a   Houde, Damian ^a   Blum, Andy ^a   Carlage, Tyler ^a   Lyubarskaya, Yelena ^a  

^a BIOGEN   (United States)

Author keywords

Biopharmaceutical process development; Charge heterogeneity; Imaging capillary IEF; Recombinant monoclonal antibody; Recombinant proteins

Indexed keywords

IMAGE PROCESSING; MONOCLONAL ANTIBODIES; QUALITY CONTROL;

BIOPHARMACEUTICAL PROCESS DEVELOPMENT; BIOPHARMACEUTICALS; CHARGE HETEROGENEITY; CONTROL ENVIRONMENT; IMAGING CAPILLARY ICIEF; PROCESS DEVELOPMENT; PROTEIN CHARGE; PROTEIN ISOFORMS; RECOMBINANT MONOCLONAL ANTIBODY; TRADITIONAL APPROACHES;

RECOMBINANT PROTEINS;

FC RECEPTOR; HYBRID PROTEIN; ISOPROTEIN; MONOCLONAL ANTIBODY; RECOMBINANT PROTEIN;

ACCURACY; ACIDITY; ANALYTIC METHOD; ANION EXCHANGE CHROMATOGRAPHY; ARTICLE; CAPILLARY ISOELECTRIC FOCUSING; CONTROLLED STUDY; CORRELATION ANALYSIS; ELECTROSPRAY MASS SPECTROMETRY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; INTERMETHOD COMPARISON; ION EXCHANGE CHROMATOGRAPHY; PROTEIN ANALYSIS; PROTEIN STABILITY; QUALITY CONTROL; QUANTITATIVE ANALYSIS; REPRODUCIBILITY; SURFACE CHARGE; TIME;

EID: 59249095069     PISSN: 01730835     EISSN: 15222683     Source Type: Journal    
DOI: 10.1002/elps.200800157     Document Type: Article

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