Rational recombinant XMRV antigen preparation and bead coupling for multiplex serology in a suspension array

Protein Expression and Purification

Volumn 80, Issue 2, 2011, Pages 176-184

  Sheikholvaezin, Ali ^a   Blomberg, Fredrik ^a   Öhrmalm, Christina ^a   Sjösten, Anna ^a   Blomberg, Jonas ^a  

^a UPPSALA UNIVERSITY   (Sweden)

Author keywords

Chronic fatigue syndrome; Diagnostic test; Myalgic encephalomyelitis; Prostate cancer; Recombinant proteins; Serology; XMRV

Indexed keywords

ANTISERUM; CARRIER PROTEIN; ESCHERICHIA COLI PROTEIN; GAG PROTEIN; HYBRID PROTEIN; THIOREDOXIN; TRXA PROTEIN, E COLI; VIRUS ANTIBODY; VIRUS ANTIGEN; VIRUS ENVELOPE PROTEIN;

AMINO ACID SEQUENCE; ANIMAL; ANTIBODY SPECIFICITY; ARTICLE; CELL INCLUSION; CYTOPLASM; ESCHERICHIA COLI; GENE VECTOR; GENETICS; HUMAN; IMMUNOLOGY; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; PLASMID; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN DENATURATION; SEROLOGY; SOLUBILITY; XENOTROPIC MULV RELATED VIRUS;

AMINO ACID SEQUENCE; ANIMALS; ANTIBODIES, VIRAL; ANTIBODY SPECIFICITY; ANTIGENS, VIRAL; CARRIER PROTEINS; CLONING, MOLECULAR; CYTOPLASM; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENE PRODUCTS, ENV; GENE PRODUCTS, GAG; GENETIC VECTORS; HUMANS; IMMUNE SERA; INCLUSION BODIES; MOLECULAR SEQUENCE DATA; PLASMIDS; PROTEIN DENATURATION; RECOMBINANT FUSION PROTEINS; SEROLOGIC TESTS; SOLUBILITY; THIOREDOXINS; XENOTROPIC MURINE LEUKEMIA VIRUS-RELATED VIRUS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; FUNGI; XENOTROPIC MURINE LEUKEMIA VIRUS;

EID: 80052853794     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2011.08.007     Document Type: Article

References (35)

1. A. Urisman, R.J. Molinaro, N. Fischer, S.J. Plummer, G. Casey, E.A. Klein, K. Malathi, C. Magi-Galluzzi, R.R. Tubbs, D. Ganem, R.H. Silverman, and J.L. DeRisi Identification of a novel Gammaretrovirus in prostate tumors of patients homozygous for R462Q RNASEL variant PLoS Pathog. 2 2006 e25
2. V.C. Lombardi, F.W. Ruscetti, J. Das Gupta, M.A. Pfost, K.S. Hagen, D.L. Peterson, S.K. Ruscetti, R.K. Bagni, C. Petrow-Sadowski, B. Gold, M. Dean, R.H. Silverman, and J.A. Mikovits Detection of an infectious retrovirus. XMRV, in blood cells of patients with chronic fatigue syndrome Science 326 2009 585 589
3. N. Rosenberg, and P. Jolicoeur Retroviral Pathogenesis J.M. Coffin, S.H. Hughes, H.E. Varmus, Retroviruses 1997 Cold Spring Harbor Laboratory Press Cold Spring Harbor 475 585 (Chapter 11)
4. R.A. Weiss The discovery of endogenous retroviruses Retrovirology 3 2006 67
5. X. Qiu, P. Swanson, K.C. Luk, B. Tu, F. Villinger, J. Das Gupta, R.H. Silverman, E.A. Klein, S. Devare, G. Schochetman, and J. Hackett Jr., Characterization of antibodies elicited by XMRV infection and development of immunoassays useful for epidemiologic studies. Retrovirology 7 2010 68
6. F. Baneyx Recombinant protein expression in Escherichia coli Curr. Opin. Biotechnol. 10 1999 411 421 (Pubitemid 29457264)
7. D.B. Smith, and K.S. Johnson Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase Gene 67 1988 31 40
8. H. Bedouelle, and P. Duplay Production in Escherichia coli and one-step purification of bifunctional hybrid proteins which bind maltose. Export of the Klenow polymerase into the periplasmic space Eur. J. Biochem. 171 1988 541 549
9. B. Nilsson, T. Moks, B. Jansson, L. Abrahmsen, A. Elmblad, E. Holmgren, C. Henrichson, T.A. Jones, and M. Uhlen A synthetic IgG-binding domain based on staphylococcal protein A Protein Eng. 1 1987 107 113
10. G.D. Davis, C. Elisee, D.M. Newham, and R.G. Harrison New fusion protein systems designed to give soluble expression in Escherichia coli Biotechnol. Bioeng. 65 1999 382 388 (Pubitemid 29482993)
11. J.R. Huth, C.A. Bewley, B.M. Jackson, A.G. Hinnebusch, G.M. Clore, and A.M. Gronenborn Design of an expression system for detecting folded protein domains and mapping macromolecular interactions by NMR Protein Sci. 6 1997 2359 2364 (Pubitemid 27490746)
12. E.R. LaVallie, E.A. DiBlasio, S. Kovacic, K.L. Grant, P.F. Schendel, and J.M. McCoy A thioredoxin gene fusion expression system that circumvents inclusion body formation in the E. coli cytoplasm Biotechnology (N. Y.) 11 1993 187 193 (Pubitemid 23056045)
13. L.A. Collins-Racie, J.M. McColgan, K.L. Grant, E.A. DiBlasio-Smith, J.M. McCoy, and E.R. LaVallie Production of recombinant bovine enterokinase catalytic subunit in Escherichia coli using the novel secretory fusion partner DsbA Biotechnology (N. Y.) 13 1995 982 987
14. D. Missiakas, C. Georgopoulos, and S. Raina The Escherichia coli dsbC (xprA) gene encodes a periplasmic protein involved in disulfide bond formation Embo J. 13 1994 2013 2020 (Pubitemid 24124535)
15. A. Zapun, D. Missiakas, S. Raina, and T.E. Creighton Structural and functional characterization of DsbC. A protein involved in disulfide bond formation in Escherichia coli Biochemistry 34 1995 5075 5089
16. E.J. Stewart, F. Aslund, and J. Beckwith Disulfide bond formation in the Escherichia coli cytoplasm: an in vivo role reversal for the thioredoxins Embo J. 17 1998 5543 5550 (Pubitemid 28445966)
17. U.K. Laemmli Cleavage of structural proteins during the assembly of the head of bacteriophage T4 Nature 227 1970 680 685
18. A. Sheikholvaezin, P. Sandstrom, D. Eriksson, N. Norgren, K. Riklund, and T. Stigbrand Optimizing the generation of recombinant single-chain antibodies against placental alkaline phosphatase Hybridoma (Larchmt) 25 2006 181 192 (Pubitemid 44744130)
19. W.K. Gillette, D. Esposito, T.E. Taylor, R.F. Hopkins, R.K. Bagni, J.L. Hartley, Purify First: rapid expression and purification of proteins from XMRV. Protein Expr Purif 76 238-247.
20. J. Koehn, and I. Hunt High-throughput protein production (HTPP): a review of enabling technologies to expedite protein production Methods Mol. Biol. 498 2009 1 18
21. R.J. Simpson, Large-scale extraction of recombinant proteins from bacteria. Cold Spring Harb Protoc 2010 pdb prot5484.
22. S.P. Qiu X, K.C. Luk, J. Gupta, N. Onlamoon, R. Silverman, F. Villinger S. Devare, G. Schochetman, J.J. Hackett, XMRV: examination of viral kinetics, Tissue Tropism, and Serological Markers of infection. (2010).
23. S. Raina, and D. Missiakas Making and breaking disulfide bonds Annu. Rev. Microbiol. 51 1997 179 202 (Pubitemid 27433047)
24. A. Rietsch, D. Belin, N. Martin, and J. Beckwith An in vivo pathway for disulfide bond isomerization in Escherichia coli Proc. Natl. Acad. Sci. USA 93 1996 13048 13053 (Pubitemid 26382767)
25. M. Sone, Y. Akiyama, and K. Ito Differential in vivo roles played by DsbA and DsbC in the formation of protein disulfide bonds J. Biol. Chem. 272 1997 10349 10352 (Pubitemid 27181032)
26. F. Baneyx, and G. Georgiou Degradation of secreted proteins in Escherichia coli Ann. N. Y. Acad. Sci. 665 1992 301 308
27. G.A. Bowden, and G. Georgiou Folding and aggregation of beta-lactamase in the periplasmic space of Escherichia coli J. Biol. Chem. 265 1990 16760 16766
28. G.A.G.G. Bowden Biotechnol. Prog. 4 1988 97 101
29. S.M. Kipriyanov, G. Moldenhauer, and M. Little High level production of soluble single chain antibodies in small-scale Escherichia coli cultures J. Immunol. Methods 200 1997 69 77 (Pubitemid 27035688)
30. A. Varshavsky The N-end rule pathway of protein degradation Genes Cells 2 1997 13 28 (Pubitemid 127688555)
31. D. Sachdev, and J.M. Chirgwin Order of fusions between bacterial and mammalian proteins can determine solubility in Escherichia coli Biochem. Biophys. Res. Commun. 244 1998 933 937 (Pubitemid 28418174)
32. F. Baneyx, and M. Mujacic Recombinant protein folding and misfolding in Escherichia coli Nat. Biotechnol. 22 2004 1399 1408
33. L.D. Cabrita, and S.P. Bottomley Protein expression and refolding-a practical guide to getting the most out of inclusion bodies Biotechnol. Annu. Rev. 10 2004 31 50 (Pubitemid 39419988)
34. O. Kolaj, S. Spada, S. Robin, and J.G. Wall Use of folding modulators to improve heterologous protein production in Escherichia coli Microb. Cell Fact 8 2009 9
35. T. Sakuma, J.M. Tonne, K.A. Squillace, S. Ohmine, T. Thatava, K.W. Peng, M.A. Barry, and Y. Ikeda Early events in retrovirus XMRV infection of the wild-derived mouse Mus pahari J. Virol. 85 2011 1205 1213