Large-scale extraction of recombinant proteins from bacteria

Cold Spring Harbor Protocols

Volumn 5, Issue 9, 2010, Pages

  Simpson, Richard J ^a  

^a NONE

Author keywords

[No Author keywords available]

Indexed keywords

ALUMINUM OXIDE; BACTERIAL PROTEIN; GLASS; LYSOZYME; RECOMBINANT PROTEIN;

ARTICLE; BACILLUS SUBTILIS; BACTERIAL CELL; BACTERIUM; CELL DISRUPTION; CELL SUSPENSION; CENTRIFUGATION; CYTOLYSIS; ESCHERICHIA COLI; EXTRACTION; GRAM NEGATIVE BACTERIUM; GRAM POSITIVE BACTERIUM; GRINDING; KLEBSIELLA PNEUMONIAE; NONHUMAN; PRESSURE CHAMBER; PROTEIN PURIFICATION; ULTRASOUND;

EID: 77958084374     PISSN: None     EISSN: 15596095     Source Type: Journal    
DOI: 10.1101/pdb.prot5484     Document Type: Article

References (7)

1. Bollag DM, Rozycki MD, and Edelstein SJ, ed. 1996. Protein methods, 2nd ed. Wiley-Liss, New York.
2. Burgess R, Jendrisak J. 1975. A procedure for the rapid, large-scale purification of Escherichia coli DNA-dependent RNA polymerase involving polymin P precipitation and DNA-cellulose chromatography. Biochemistry 14: 4634-4638.
3. Scopes RK. 1994. Protein purification: Principles and practice, 3rd ed. Springer, New York.
4. Simpson RJ. 2006. SDS-PAGE of proteins. Cold Spring Harb Protoc doi: 10.1101/pdb.prot4313.
5. Simpson RJ. 2010a. Small-scale extraction of recombinant proteins from bacteria. Cold Spring Harb Protoc (this issue). doi: 10.1101/pdb.prot5483.
6. Simpson RJ. 2010b. Solubilization of Escherichia coli recombinant proteins from inclusion bodies. Cold Spring Harb Protoc (this issue). doi: 10.1101/pdb.prot5485.
7. Worrall DM. 1996. Extraction of recombinant protein from bacteria. Methods Mol Biol 59: 31-37.