A combined approach of classical mutagenesis and rational metabolic engineering improves rapamycin biosynthesis and provides insights into methylmalonyl-CoA precursor supply pathway in Streptomyces hygroscopicus ATCC 29253

Applied Microbiology and Biotechnology

Volumn 91, Issue 5, 2011, Pages 1389-1397

  Jung, Won Seok ^a   Yoo, Young Ji ^a   Park, Je Won ^b   Park, Sung Ryeol ^a   Han, Ah Reum ^a   Ban, Yeon Hee ^a   Kim, Eun Ji ^a   Kim, Eunji ^a   Yoon, Yeo Joon ^a  

^a Ewha Womans University   (South Korea)

^b SunMoon University   (South Korea)

Author keywords

Metabolic engineering; Methylmalonyl CoA; Propionyl CoA carboxylase; Random mutagenesis; Rapamycin

Indexed keywords

METABOLIC ENGINEERING; METHYLMALONYL-COA; PROPIONYL-COA CARBOXYLASE; RANDOM MUTAGENESIS; RAPAMYCIN;

BACTERIA; BIOCHEMISTRY; BIOSYNTHESIS; DRIERS (MATERIALS); GENE EXPRESSION; KETONES; LAKES; METABOLISM;

MUTAGENESIS;

METHYLMALONYL COENZYME A CARBOXYLTRANSFERASE; METHYLMALONYL COENZYME A MUTASE; PROPIONIC ACID; PROPIONYL COENZYME A CARBOXYLASE; RAPAMYCIN;

BACTERIUM; BIOENGINEERING; COMPARATIVE STUDY; ENZYME ACTIVITY; GENE EXPRESSION; IMMUNOASSAY; SECONDARY METABOLITE;

ARTICLE; BIOSYNTHESIS; CONTROLLED STUDY; GENE EXPRESSION; GENETIC ANALYSIS; GENETIC MANIPULATION; METABOLIC ENGINEERING; MUTAGENESIS; NONHUMAN; STREPTOMYCES HYGROSCOPICUS; SUPPLEMENTATION;

ACYL COENZYME A; BACTERIAL PROTEINS; BIOSYNTHETIC PATHWAYS; GENE EXPRESSION REGULATION, BACTERIAL; GENETIC ENGINEERING; METHYLMALONYL-COA DECARBOXYLASE; METHYLMALONYL-COA MUTASE; MUTAGENESIS; SIROLIMUS; STREPTOMYCES;

STREPTOMYCES HYGROSCOPICUS;

EID: 80052611955     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-011-3348-6     Document Type: Article

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