Genetic methods and strategies for secondary metabolite yield improvement in actinomycetes

Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology

Volumn 79, Issue 3-4, 2001, Pages 251-259

  Baltz, R H ^a  

^a CognoGen Biotechnology Consulting ^*  (United States)

Author keywords

Gene duplication; Microbial genomics; Mutagenesis; Protoplast fusion; Transposition

Indexed keywords

BIOTECHNOLOGY; SECONDARY METABOLITE;

ACTINOMYCES; ARTICLE; BACTERIAL CHROMOSOME; BACTERIAL METABOLISM; BACTERIUM MUTANT; BIOSYNTHESIS; CONTROLLED STUDY; FERMENTATION; GENE DISRUPTION; GENE DUPLICATION; GENE FREQUENCY; GENE FUSION; GENE INSERTION; GENETIC ANALYSIS; GENETIC RECOMBINATION; METABOLITE; MODEL; MOLECULAR CLONING; MOLECULAR GENETICS; MUTAGENESIS; NONHUMAN; PRIORITY JOURNAL; PROBABILITY; PROMOTER REGION; PROTOPLAST; RANDOMIZATION; REGULATOR GENE; REGULATORY MECHANISM; REPRODUCIBILITY; STRUCTURAL GENE; TRANSPOSON;

ACTINOMYCETALES; BACTERIAL PROTEINS; DNA TRANSPOSABLE ELEMENTS; GENETIC ENGINEERING; GENOME, BACTERIAL; GENOMICS; MUTAGENESIS; RECOMBINATION, GENETIC;

EID: 0035149952     PISSN: 00036072     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1012020918624     Document Type: Article

References (49)

1. Genetic recombination in Streptomyces fradiae by protoplast fusion and cell regeneration
2. Mutagenesis in Streptomyces
3. Mutation in Streptomyces
4. Gene expression in recombinant Streptomyces
5. Molecular genetic approaches to yield improvement in actinomycetes
6. Genetic manipulation of antibiotic producing Streptomyces
7. New genetic methods to improve secondary metabolite production in Streptomyces
8. Mutagenesis
9. Mutagenesis
10. Molecular genetic methods for improving secondary-metabolite production in actinomycetes
11. Protoplast fusion in Streptomyces: Conditions for efficient genetic recombination and cell regeneration
12. Advances in protoplast fusion and transformation in Streptomyces
13. Mutagenic and error-free DNA repair in Streptomyces
14. Adaptive response and enhancement of N-methyl-N′-nitro-N-nitrosoguanidine mutagenesis by chloramphenicol in Streptomyces fradiae
15. Transposition of Tn5096 and related transposons in Streptomyces species
16. Applications of transposition mutagenesis in antibiotic producing streptomycetes
17. Properties of transposons derived from IS493 and applications in streptomycetes
18. The regulation of antibiotic production in Streptomyces coelicolor A3(2)
19. Genetic studies of the lac repressor IV. Mutagenic specificity in the lacl gene of Escherichia coli
20. Bacterial transcript imaging by hybridization of total RNA to oligonucleotide arrays
21. The 387 kb linear plasmid of Streptomyces rimosus and its interactions with the chromosome
22. Tn5099, a xylE promoter probe transposon for Streptomyces spp
23. A novel method for improving Streptomyces coelicolor A3(2) for production of actinorhodin by introduction of rpsL (encoding ribosomal protein S12) mutations conferring resistance to streptomycin
24. Acquisition of certain streptomycin-resistant (str) mutations enhances antibiotic production in bacteria
25. Mutants of Streptomyces roseosporus that express enhanced recombination within partially homologous genes
26. Use of rpsL for dominance selection and gene replacement in Streptomyces roseosporus
27. Protoplast fusion
28. Conjugal transfer of cosmid DNA from Escherichia coli to Saccharopolyspora spinosa: Effects of chromosomal insertions on macrolide A83543 production
29. Gene transfer and transposition mutagenesis in Streptomyces roseosporus: Mapping of insertions that influence daptomycin or pigment production
30. Mutational specificity in bacteria
31. Spontaneous mutators in bacteria
32. The use of gene clusters to infer functional coupling
33. Representation of function: The next step
34. Molecular characterization of the lincomycin-production gene cluster of Streptomyces lincolnensis 78-11
35. Screening and selection for strain improvement
36. AT-CG transversions and their prevention by the Escherichia coli mutT and mutHLS pathways
37. Toward metabolic phenomics: Analysis of genomic data using flux balances
38. MPWW: The metabolic pathways database
39. A reconstruction of the metabolism of Methanococcus jannaschii
40. Complete conversion of antibiotic precursor to pristinamycin IIA by overexpression of Streptomyces pristinaespiralis biosynthetic genes
41. Induction of actinorhodin production by rpsL (encoding ribosomal protein S12) mutations that confer streptomycin resistance in Streptomyces coelicolor A3(2)
42. Transposition of Tn5096 and other IS493 derivatives in Streptomyces griseofuscus
43. Hyper-transposing derivatives of the streptomycete insertion sequence IS493
44. Transposition mutagenesis in Streptomyces fradiae: Identification of a neutral site for the stable insertion of DNA by transposon exchange
45. Mutagenic DNA repair in Streptomyces
46. Functional genomics: Expression analysis of Escherichia coli growing on minimal and rich media
47. Diagnosis of cellular states of microbial organisms using proteomics
48. Strain improvement by nonrecombinant methods
49. Codon usage in the G+C-rich Streptomyces genome