Proteomic screening method for phosphopeptide motif binding proteins using peptide libraries

Journal of Proteome Research

Volumn 10, Issue 9, 2011, Pages 4158-4164

  Christofk, Heather R ^a,c   Wu, Ning ^a,b   Cantley, Lewis C ^a,b   Asara, John M ^a,b  

^a HARVARD MEDICAL SCHOOL   (United States)

^b BETH ISRAEL DEACONESS MEDICAL CENTER   (United States)

^c UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

HeLa Henrietta Lacks cells; Pfam protein families database; pSer phosphoserine; pThr phosphothreonine; pTyr phosphotyrosine; SH2 Src homology 2; SH3 Src homology 3; SILAC stable isotope labeling of amino acids in cell culture

Indexed keywords

ASPARTIC ACID; BINDING PROTEIN; GLUTAMIC ACID; PEPTIDE LIBRARY; PEPTIDYLPROLYL ISOMERASE PIN1; PHOSPHATIDYLINOSITOL 3 KINASE; PHOSPHOPEPTIDE; PHOSPHOTRANSFERASE; POLO LIKE KINASE 1; PROLINE; PROTEIN 14 3 3; PROTEIN P85; PROTEIN SH2; PROTEIN TYROSINE PHOSPHATASE; PYRUVATE KINASE; PYRUVATE KINASE M2; SERINE; STAT PROTEIN; TYROSINE; UNCLASSIFIED DRUG;

ANALYTIC METHOD; ARTICLE; CELL LYSATE; CONTROLLED STUDY; HUMAN; HUMAN CELL; HYBRID; LIQUID CHROMATOGRAPHY; MASS SPECTROMETER; PRIORITY JOURNAL; PROTEIN ASSEMBLY; PROTEIN DOMAIN; PROTEIN MOTIF; PROTEIN PHOSPHORYLATION; PROTEOMICS; QUADRUPOLE MASS SPECTROMETRY; SCREENING TEST; SIGNAL TRANSDUCTION; TANDEM MASS SPECTROMETRY;

AMINO ACID SEQUENCE; BINDING SITES; HELA CELLS; HUMANS; ISOTOPE LABELING; MASS SPECTROMETRY; MOLECULAR SEQUENCE DATA; PEPTIDE LIBRARY; PHOSPHOAMINO ACIDS; PHOSPHOPEPTIDES; PROTEIN BINDING; PROTEINS; PROTEOMICS; SRC HOMOLOGY DOMAINS;

EID: 80052510197     PISSN: 15353893     EISSN: 15353907     Source Type: Journal    
DOI: 10.1021/pr200578n     Document Type: Article

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