Proteomic screen finds pSer/pThr-binding domain localizing Plk1 to mitotic substrates

Science

Volumn 299, Issue 5610, 2003, Pages 1228-1231

  Elia, Andrew E H ^a,b   Cantley, Lewis C ^b   Yaffe, Michael B ^a  

^a MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

^b BETH ISRAEL DEACONESS MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CELLS; ENZYMES; SUBSTRATES;

PROTEOMIC SCREENS;

ENZYME KINETICS;

CYCLIN DEPENDENT KINASE; PHOSPHOPEPTIDE; PHOSPHOSERINE; PHOSPHOTHREONINE; PHOSPHOTRANSFERASE; POLO LIKE KINASE 1; UNCLASSIFIED DRUG;

BIOCHEMISTRY; PROTEIN;

ARTICLE; CENTROSOME; ENZYME PHOSPHORYLATION; MITOSIS; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN DOMAIN; PROTEIN LOCALIZATION; PROTEIN MOTIF; PROTEOMICS; PROTEROZOIC; SIGNAL TRANSDUCTION;

AMINO ACID MOTIFS; BINDING SITES; CALORIMETRY; CDC25 PHOSPHATASE; CELL CYCLE PROTEINS; CENTROSOME; HELA CELLS; HUMANS; LIGANDS; MITOSIS; PEPTIDE LIBRARY; PHOSPHOPEPTIDES; PHOSPHORYLATION; PHOSPHOSERINE; PHOSPHOTHREONINE; POINT MUTATION; PROTEIN BINDING; PROTEIN KINASES; PROTEIN STRUCTURE, TERTIARY; PROTEIN-SERINE-THREONINE KINASES; PROTEOMICS; PROTO-ONCOGENE PROTEINS; SIGNAL TRANSDUCTION;

EID: 0242515843     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.1079079     Document Type: Article

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25. We thank F. Kanai and P. A. Marignani for providing cDNA plasmid pools used in the screen, F. McKeon for providing the Cdc25 construct, and members of the M.B.Y. and L.C.C. laboratories for technical assistance and helpful discussions. Supported by National Institutes of Health grants GM52981 (M.B.Y.) and GM56203 (L.C.C.) and a Burroughs-Wellcome Career Development Award (M.B.Y.).