KARP-1 works as a heterodimer with Ku70, but the function of KARP-1 cannot perfectly replace that of Ku80 in DSB repair

Experimental Cell Research

Volumn 317, Issue 16, 2011, Pages 2267-2275

  Koike, Manabu ^a   Yutoku, Yasutomo ^a,b   Koike, Aki ^a  

^a NATIONAL INSTITUTE OF RADIOLOGICAL SCIENCES   (Japan)

^b CHIBA UNIVERSITY   (Japan)

Author keywords

DNA repair; KARP 1; Ku70; Ku80; Nuclear localization signal; Radiation

Indexed keywords

HETERODIMER; KU ANTIGEN; PROTEIN KARP 1; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CARBOXY TERMINAL SEQUENCE; CELL LINE; CONTROLLED STUDY; DOUBLE STRANDED DNA BREAK; HUMAN; MOUSE; NONHUMAN; NUCLEAR LOCALIZATION SIGNAL; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FUNCTION; RADIOSENSITIVITY;

PRIMATES; RODENTIA;

EID: 80051794910     PISSN: 00144827     EISSN: 10902422     Source Type: Journal    
DOI: 10.1016/j.yexcr.2011.06.015     Document Type: Article

References (28)

1. Khanna K.K., Jackson S.P. DNA double-strand breaks: signaling, repair and the cancer connection. Nat. Genet. 2001, 27:247-254.
2. Shiloh Y. ATM and related protein kinases: safeguarding genome integrity. Nat. Rev. Cancer 2003, 3:155-168.
3. Mahaney B.L., Meek K., Lees-Miller S.P. Repair of ionizing radiation-induced DNA double-strand breaks by non-homologous end-joining. Biochem. J. 2009, 417:639-650.
4. Featherstone C., Jackson S.P. Ku, a DNA repair protein with multiple cellular functions?. Mutat. Res. 1999, 434:3-15.
5. Tuteja R., Tuteja N. Ku autoantigen: a multifunctional DNA-binding protein. Crit. Rev. Biochem. Mol. Biol. 2000, 35:1-33.
6. Downs J.A., Jackson S.P. A means to a DNA end: the many roles of Ku. Nat. Rev. Mol. Cell Biol. 2004, 5:367-378.
7. Myung K., He D.M., Lee S.E., Hendrickson E.A. KARP-1: a novel leucine zipper protein expressed from the Ku86 autoantigen locus is implicated in the control of DNA-dependent protein kinase activity. EMBO J. 1997, 16:3172-3184.
8. Koike M. Dimerization, translocation and localization of Ku70 and Ku80 proteins. J. Radiat. Res. 2002, 43:223-236.
9. Singleton B.K., Priestley A., Steingrimsdottir H., Gell D., Blunt T., Jackson S.P., Lehmann A.R., Jeggo P.A. Molecular and biochemical characterization of xrs mutants defective in Ku80. Mol. Cell. Biol. 1997, 17:1264-1273.
10. Koike M., Shiomi T., Koike A. Ku70 can translocate to the nucleus independent of Ku80 translocation and DNA-PK autophosphorylation. Biochem. Biophys. Res. Commun. 2000, 276:1105-1111.
11. Koike M., Miyasaka T., Mimori T., Shiomi T. Subcellular localization and protein-protein interaction regions of Ku proteins. Biochem. Biophys. Res. Commun. 1998, 252:679-685.
12. Koike M., Koike A. Establishment and characterization of stable cell lines expressing human Ku80 tagged with enhanced green fluorescent protein. J. Radiat. Res. 2004, 45:119-125.
13. Koike M., Shiomi T., Koike A. Dimerization and nuclear localization of Ku proteins. J. Biol. Chem. 2001, 276:11167-11173.
14. Koike M., Ikuta T., Miyasaka T., Shiomi T. Ku80 can translocate to the nucleus independent of the translocation of Ku70 using its own nuclear localization signal. Oncogene 1999, 18:7495-7505.
15. Koike M., Ikuta T., Miyasaka T., Shiomi T. The nuclear localization signal of the human Ku70 is a variant bipartite type recognized by the two components of nuclear pore-targeting complex. Exp. Cell Res. 1999, 250:401-413.
16. Koike M., Koike A. Accumulation of Ku80 proteins at DNA double-strand breaks in living cells. Exp. Cell Res. 2008, 314:1061-1070.
17. Koike M., Awaji T., Kataoka M., Tsujimoto G., Kartasova T., Koike A., Shiomi T. Differential subcellular localization of DNA-dependent protein kinase components Ku and DNA-PKcs during mitosis. J. Cell Sci. 1999, 112:4031-4039.
18. Taccioli G.E., Gottlieb T.M., Blunt T., Priestley A., Demengeot J., Mizuta R., Lehmann A.R., Alt F.W., Jackson S.P., Jeggo P.A. Ku80: product of the XRCC5 gene and its role in DNA repair and V(D)J recombination. Science 1994, 265:1442-1445.
19. Kanda T., Sullivan K.F., Wahl G.M. Histone-GFP fusion protein enables sensitive analysis of chromosome dynamics in living mammalian cells. Curr. Biol. 1998, 8:377-385.
20. Myung K., Braastad C., He D.M., Hendrickson E.A. KARP-1 is induced by DNA damage in a p53- and ataxia telangiectasia mutated-dependent fashion. Proc. Natl. Acad. Sci. U.S.A. 1998, 95:7664-7669.
21. Tanaka Y., Imamura J., Kanai F., Ichimura T., Isobe T., Koike M., Kudo Y., Tateishi K., Ikenoue T., Ijichi H., Yamaji Y., Yoshida H., Kawabe T., Omata M. Runx3 interacts with DNA repair protein Ku70. Exp. Cell Res. 2007, 313:3251-3260.
22. Brenkman A.B., van den Broek N.J., de Keizer P.L., van Gent D.C., Burgering B.M. The DNA damage repair protein Ku70 interacts with FOXO4 to coordinate a conserved cellular stress response. FASEB J. 2010, 24:4271-4280.
23. Pucci S., Mazzarelli P., Nucci C., Ricci F., Spagnoli L.G. CLU "in and out": looking for a link. Adv. Cancer Res. 2009, 105:93-113.
24. Marti T.M., Hefner E., Feeney L., Natale V., Cleaver J.E. H2AX phosphorylation within the G1 phase after UV irradiation depends on nucleotide excision repair and not DNA double-strand breaks. Proc. Natl. Acad. Sci. U. S. A. 2006, 103:9891-9896.
25. Quanz M., Chassoux D., Berthault N., Agrario C., Sun J.-S., Dutreix M. Hyperactivation of DNA-PK by double-strand break mimicking molecules disorganizes DNA damage response. PLoS One 2009, 4:e6298.
26. McManus K.J., Hendzel M.J. ATM-dependent DNA damage-independent mitotic phosphorylation of H2AX in normally growing mammalian cells. Mol. Biol. Cell 2005, 16:5013-5025.
27. Ichijima Y., Sakasai R., Okita N., Asahina K., Mizutani S., Teraoka H. Phosphorylation of histone H2AX at M phase in human cells without DNA damage response. Biochem. Biophys. Res. Commun. 2005, 336:807-812.
28. Kinner A., Wu W., Staudt C., Iliakis G. Gamma-H2AX in recognition and signaling of DNA double-strand breaks in the context of chromatin. Nucleic Acids Res. 2008, 36:5678-5694.