Improved thermostability and PCR efficiency of Thermococcus celericrescens DNA polymerase via site-directed mutagenesis

Journal of Biotechnology

Volumn 155, Issue 2, 2011, Pages 156-163

  Kim, Kee Pum ^a   Cho, Sung Suk ^a   Lee, Kang Keun ^a   Youn, Man Hui ^a   Kwon, Suk Tae ^a  

^a Sungkyunkwan University   (South Korea)

Author keywords

PCR amplification; Polymerase chain reaction (PCR); Tcel DNA polymerase; TcelA752K N213D DNA polymerase; Thermococcus celericrescens (Tcel); Thermostability

Indexed keywords

PCR AMPLIFICATION; TCEL DNA POLYMERASE; TCELA752K/N213D DNA POLYMERASE; THERMOCOCCUS CELERICRESCENS (TCEL); THERMOSTABILITY;

AMINO ACIDS; COLUMN CHROMATOGRAPHY; DNA; EFFICIENCY; ENZYMES; ESCHERICHIA COLI; GENES; MUTAGENESIS; POLYMERS;

POLYMERASE CHAIN REACTION;

BACTERIAL ENZYME; DNA POLYMERASE; EXONUCLEASE; MUTANT PROTEIN;

ARTICLE; COLUMN CHROMATOGRAPHY; CONTROLLED STUDY; ENZYME STABILITY; ESCHERICHIA COLI; GENE; GENE AMPLIFICATION; HEAT TREATMENT; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN MOTIF; SITE DIRECTED MUTAGENESIS; THERMOCOCCUS; THERMOCOCCUS CELERICRESCENS; THERMOSTABILITY; WILD TYPE;

AMINO ACID SUBSTITUTION; BASE SEQUENCE; CLONING, MOLECULAR; DNA PRIMERS; DNA-DIRECTED DNA POLYMERASE; ESCHERICHIA COLI; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS, DNA; TEMPERATURE; THERMOCOCCUS;

ESCHERICHIA COLI; THERMOCOCCUS;

EID: 80051472159     PISSN: 01681656     EISSN: 18734863     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2011.06.022     Document Type: Article

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