Cloning, expression, and PCR application of DNA polymerase from the hyperthermophilic archaeon, Thermococcus celer

Biotechnology Letters

Volumn 33, Issue 2, 2011, Pages 339-346

  Kim, Kee Pum ^a   Bae, Heejin ^a   Kim, In Hye ^a   Kwon, Suk Tae ^a  

^a Sungkyunkwan University   (South Korea)

Author keywords

DNA polymerase; PCR improvement; Thermococcus celer

Indexed keywords

AMINO ACID RESIDUES; DEGENERATE PRIMERS; DNA POLYMERASE; DNA POLYMERASE GENES; DNA WALKING; GENOMIC DNA; HYPERTHERMOPHILIC ARCHAEON; OPTIMAL CONDITIONS; PCR IMPROVEMENT; THERMOCOCCUS CELER;

AMINO ACIDS; CLONING; COLUMN CHROMATOGRAPHY; DNA; GENE ENCODING; POLYMERS;

POLYMERASE CHAIN REACTION;

ARCHAEAL DNA; ARCHAEAL PROTEIN; DNA DIRECTED DNA POLYMERASE;

AFFINITY CHROMATOGRAPHY; ARTICLE; CHEMISTRY; DNA SEQUENCE; ENZYMOLOGY; GENETICS; HEAT; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; MOLECULAR CLONING; MOLECULAR GENETICS; MOLECULAR WEIGHT; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; POLYMERASE CHAIN REACTION; THERMOCOCCUS;

ARCHAEAL PROTEINS; CHROMATOGRAPHY, AFFINITY; CLONING, MOLECULAR; DNA, ARCHAEAL; DNA-DIRECTED DNA POLYMERASE; HOT TEMPERATURE; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; OPEN READING FRAMES; POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS, DNA; THERMOCOCCUS;

ARCHAEA; THERMOCOCCUS; THERMOCOCCUS CELER;

EID: 78651425188     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-010-0434-2     Document Type: Article

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