Cloning, purification, and characterization of a new DNA polymerase from a hyperthermophilic archaeon, Thermococcus sp. NA1

Journal of Microbiology and Biotechnology

Volumn 17, Issue 7, 2007, Pages 1090-1097

  Kim, Yun Jae ^a,b   Lee, Hyun Sook ^a   Bae, Seung Seob ^a   Jeon, Jeong Ho ^a   Lim, Jae Kyu ^a   Cho, Yona ^a   Nam, Ki Hoon ^b   Kang, Sung Gyun ^a   Kim, Sang Jin ^a   Kwon, Suk Tae ^b   Lee, Jung Hyun ^a  

^a Korea Research Institute of Ships and Ocean Engineering   (South Korea)

^b Sungkyunkwan University   (South Korea)

Author keywords

Characterization; Family B type DNA polymerase; Fidelity; Hyperthermophile; Processivity

Indexed keywords

DNA POLYMERASE; KOD POLYMERASE; NUCLEOTIDYLTRANSFERASE; PHENFORMIN; TAQ POLYMERASE; THERMOCOCCUS NA1 POLYMERASE; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL GENETICS; BIOTECHNOLOGICAL PRODUCTION; CHROMATOGRAPHY; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME PURIFICATION; GENE OVEREXPRESSION; HALF LIFE TIME; HYPERTHERMOPHILIC ARCHAEON; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PH; POLYMERASE CHAIN REACTION; PROCESSING; THERMOCOCCUS; THERMOSTABILITY;

AMINO ACID MOTIFS; AMINO ACID SEQUENCE; ARCHAEA; ARCHAEAL PROTEINS; BASE SEQUENCE; CLONING, MOLECULAR; DNA PRIMERS; DNA, BACTERIAL; DNA-DIRECTED DNA POLYMERASE; ENZYME STABILITY; ESCHERICHIA COLI; GENES, BACTERIAL; HALF-LIFE; HYDROGEN-ION CONCENTRATION; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; OPEN READING FRAMES; POLYMERASE CHAIN REACTION; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT PROTEINS; SEQUENCE ANALYSIS, DNA; TEMPERATURE; THERMOCOCCUS;

ARCHAEA; ESCHERICHIA COLI; THERMOCOCCUS SP.;

EID: 34547987644     PISSN: 10177825     EISSN: 17388872     Source Type: Journal    
DOI: None     Document Type: Article

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