-
1
-
-
40049099087
-
Microscale fluorescent thermal stability assay for membrane proteins
-
Alexandrov A.I., Mileni M., Chien E.Y., Hanson M.A., Stevens R.C. Microscale fluorescent thermal stability assay for membrane proteins. Structure 2008, 16:351-359.
-
(2008)
Structure
, vol.16
, pp. 351-359
-
-
Alexandrov, A.I.1
Mileni, M.2
Chien, E.Y.3
Hanson, M.A.4
Stevens, R.C.5
-
2
-
-
0027016842
-
Dissection of protein structure and folding by directed mutagenesis
-
Baase W.A., Eriksson A.E., Zhang X., Heinz D.W., Sauer U., Blaber M., Baldwin E.P., Wozniak J.A., Matthews B.W. Dissection of protein structure and folding by directed mutagenesis. Faraday Discuss. 1992, 173-181.
-
(1992)
Faraday Discuss.
, pp. 173-181
-
-
Baase, W.A.1
Eriksson, A.E.2
Zhang, X.3
Heinz, D.W.4
Sauer, U.5
Blaber, M.6
Baldwin, E.P.7
Wozniak, J.A.8
Matthews, B.W.9
-
3
-
-
0027016842
-
Dissection of protein structure and folding by directed mutagenesis
-
Baase W.A., Eriksson A.E., Zhang X.J., Heinz D.W., Sauer U., Blaber M., Baldwin E.P., Wozniak J.A., Matthews B.W. Dissection of protein structure and folding by directed mutagenesis. Faraday Discuss. 1992, 173-181.
-
(1992)
Faraday Discuss.
, pp. 173-181
-
-
Baase, W.A.1
Eriksson, A.E.2
Zhang, X.J.3
Heinz, D.W.4
Sauer, U.5
Blaber, M.6
Baldwin, E.P.7
Wozniak, J.A.8
Matthews, B.W.9
-
4
-
-
26444492138
-
Recent advances in GFP folding reporter and split-GFP solubility reporter technologies. Application to improving the folding and solubility of recalcitrant proteins from Mycobacterium tuberculosis
-
Cabantous S., Pedelacq J.D., Mark B.L., Naranjo C., Terwilliger T.C., Waldo G.S. Recent advances in GFP folding reporter and split-GFP solubility reporter technologies. Application to improving the folding and solubility of recalcitrant proteins from Mycobacterium tuberculosis. J. Struct. Funct. Genomics 2005, 6:113-119.
-
(2005)
J. Struct. Funct. Genomics
, vol.6
, pp. 113-119
-
-
Cabantous, S.1
Pedelacq, J.D.2
Mark, B.L.3
Naranjo, C.4
Terwilliger, T.C.5
Waldo, G.S.6
-
5
-
-
79955021788
-
Evolution of three human GPCRs for higher expression and stability
-
Dodevski I., Pluckthun A. Evolution of three human GPCRs for higher expression and stability. J. Mol. Biol. 2011, 408:599-615.
-
(2011)
J. Mol. Biol.
, vol.408
, pp. 599-615
-
-
Dodevski, I.1
Pluckthun, A.2
-
6
-
-
33645454462
-
Optimization of membrane protein overexpression and purification using GFP fusions
-
Drew D., Gier J. Optimization of membrane protein overexpression and purification using GFP fusions. Nat. Methods 2006, 3:303.
-
(2006)
Nat. Methods
, vol.3
, pp. 303
-
-
Drew, D.1
Gier, J.2
-
7
-
-
43149098999
-
GFP-based optimization scheme for the overexpression and purification of eukaryotic membrane proteins in Saccharomyces cerevisiae
-
Drew D., Newstead S., Sonoda Y., Kim H., von Heijne G., Iwata S. GFP-based optimization scheme for the overexpression and purification of eukaryotic membrane proteins in Saccharomyces cerevisiae. Nat. Protoc. 2008, 3:784-798.
-
(2008)
Nat. Protoc.
, vol.3
, pp. 784-798
-
-
Drew, D.1
Newstead, S.2
Sonoda, Y.3
Kim, H.4
von Heijne, G.5
Iwata, S.6
-
8
-
-
23644458922
-
A scalable, GFP-based pipeline for membrane protein overexpression screening and purification
-
Drew D., Slotboom D.J., Friso G., Reda T., Genevaux P., Rapp M., Meindl-Beinker N.M., Lambert W., Lerch M., Daley D.O., et al. A scalable, GFP-based pipeline for membrane protein overexpression screening and purification. Protein Sci. 2005, 14:2011-2017.
-
(2005)
Protein Sci.
, vol.14
, pp. 2011-2017
-
-
Drew, D.1
Slotboom, D.J.2
Friso, G.3
Reda, T.4
Genevaux, P.5
Rapp, M.6
Meindl-Beinker, N.M.7
Lambert, W.8
Lerch, M.9
Daley, D.O.10
-
9
-
-
33748929006
-
Thermofluor-based high-throughput stability optimization of proteins for structural studies
-
Ericsson U.B., Hallberg B.M., Detitta G.T., Dekker N., Nordlund P. Thermofluor-based high-throughput stability optimization of proteins for structural studies. Anal. Biochem. 2006, 357:289-298.
-
(2006)
Anal. Biochem.
, vol.357
, pp. 289-298
-
-
Ericsson, U.B.1
Hallberg, B.M.2
Detitta, G.T.3
Dekker, N.4
Nordlund, P.5
-
10
-
-
79955612777
-
An efficient strategy for high throughput screening of recombinant integral membrane protein expression and stability
-
Fan J., Heng J., Dai S., Shaw N., Zhou B., Huang B., He Z., Wang Y., Jiang T., Li X., et al. An efficient strategy for high throughput screening of recombinant integral membrane protein expression and stability. Protein Expr. Purif. 2011, 78:6-13.
-
(2011)
Protein Expr. Purif.
, vol.78
, pp. 6-13
-
-
Fan, J.1
Heng, J.2
Dai, S.3
Shaw, N.4
Zhou, B.5
Huang, B.6
He, Z.7
Wang, Y.8
Jiang, T.9
Li, X.10
-
11
-
-
77953347438
-
MARINE-EXPRESS: taking advantage of high throughput cloning and expression strategies for the post-genomic analysis of marine organisms
-
Groisillier A., Herve C., Jeudy A., Rebuffet E., Pluchon P.F., Chevolot Y., Flament D., Geslin C., Morgado I.M., Power D., et al. MARINE-EXPRESS: taking advantage of high throughput cloning and expression strategies for the post-genomic analysis of marine organisms. Microb. Cell Fact. 2010, 9:45.
-
(2010)
Microb. Cell Fact.
, vol.9
, pp. 45
-
-
Groisillier, A.1
Herve, C.2
Jeudy, A.3
Rebuffet, E.4
Pluchon, P.F.5
Chevolot, Y.6
Flament, D.7
Geslin, C.8
Morgado, I.M.9
Power, D.10
-
12
-
-
34250833480
-
A high-throughput method for membrane protein solubility screening: the ultracentrifugation dispersity sedimentation assay
-
Gutmann D.A., Mizohata E., Newstead S., Ferrandon S., Postis V., Xia X., Henderson P.J., van Veen H.W., Byrne B. A high-throughput method for membrane protein solubility screening: the ultracentrifugation dispersity sedimentation assay. Protein Sci. 2007, 16:1422-1428.
-
(2007)
Protein Sci.
, vol.16
, pp. 1422-1428
-
-
Gutmann, D.A.1
Mizohata, E.2
Newstead, S.3
Ferrandon, S.4
Postis, V.5
Xia, X.6
Henderson, P.J.7
van Veen, H.W.8
Byrne, B.9
-
13
-
-
4243139636
-
Structural genomics on membrane proteins: mini review
-
Lundstrom K. Structural genomics on membrane proteins: mini review. Comb. Chem. High Throughput Screen. 2004, 7:431-439.
-
(2004)
Comb. Chem. High Throughput Screen.
, vol.7
, pp. 431-439
-
-
Lundstrom, K.1
-
14
-
-
18244380348
-
High-density miniaturized thermal shift assays as a general strategy for drug discovery
-
Pantoliano M.W., Petrella E.C., Kwasnoski J.D., Lobanov V.S., Myslik J., Graf E., Carver T., Asel E., Springer B.A., Lane P., et al. High-density miniaturized thermal shift assays as a general strategy for drug discovery. J. Biomol. Screen. 2001, 6:429-440.
-
(2001)
J. Biomol. Screen.
, vol.6
, pp. 429-440
-
-
Pantoliano, M.W.1
Petrella, E.C.2
Kwasnoski, J.D.3
Lobanov, V.S.4
Myslik, J.5
Graf, E.6
Carver, T.7
Asel, E.8
Springer, B.A.9
Lane, P.10
-
15
-
-
57049083012
-
A high-throughput assay of membrane protein stability
-
Postis V.L., Deacon S.E., Roach P.C., Wright G.S., Xia X., Ingram J.C., Hadden J.M., Henderson P.J., Phillips S.E., McPherson M.J., et al. A high-throughput assay of membrane protein stability. Mol. Membr. Biol. 2008, 25:617-624.
-
(2008)
Mol. Membr. Biol.
, vol.25
, pp. 617-624
-
-
Postis, V.L.1
Deacon, S.E.2
Roach, P.C.3
Wright, G.S.4
Xia, X.5
Ingram, J.C.6
Hadden, J.M.7
Henderson, P.J.8
Phillips, S.E.9
McPherson, M.J.10
-
16
-
-
33947172767
-
Detergents for the stabilization and crystallization of membrane proteins
-
Prive G.G. Detergents for the stabilization and crystallization of membrane proteins. Methods 2007, 41:388-397.
-
(2007)
Methods
, vol.41
, pp. 388-397
-
-
Prive, G.G.1
-
17
-
-
44449145917
-
Construction of a series of vectors for high throughput cloning and expression screening of membrane proteins from Mycobacterium tuberculosis
-
Qin H., Hu J., Hua Y., Challa S.V., Cross T.A., Gao F.P. Construction of a series of vectors for high throughput cloning and expression screening of membrane proteins from Mycobacterium tuberculosis. BMC Biotechnol. 2008, 8:51.
-
(2008)
BMC Biotechnol.
, vol.8
, pp. 51
-
-
Qin, H.1
Hu, J.2
Hua, Y.3
Challa, S.V.4
Cross, T.A.5
Gao, F.P.6
-
18
-
-
39149100948
-
Stabilization of the human beta2-adrenergic receptor TM4-TM3-TM5 helix interface by mutagenesis of Glu122(341), a critical residue in GPCR structure
-
Roth C.B., Hanson M.A., Stevens R.C. Stabilization of the human beta2-adrenergic receptor TM4-TM3-TM5 helix interface by mutagenesis of Glu122(341), a critical residue in GPCR structure. J. Mol. Biol. 2008, 376:1305-1319.
-
(2008)
J. Mol. Biol.
, vol.376
, pp. 1305-1319
-
-
Roth, C.B.1
Hanson, M.A.2
Stevens, R.C.3
-
19
-
-
54449087770
-
Directed evolution of a G protein-coupled receptor for expression, stability, and binding selectivity
-
Sarkar C.A., Dodevski I., Kenig M., Dudli S., Mohr A., Hermans E., Pluckthun A. Directed evolution of a G protein-coupled receptor for expression, stability, and binding selectivity. Proc. Natl. Acad. Sci. USA 2008, 105:14808-14813.
-
(2008)
Proc. Natl. Acad. Sci. USA
, vol.105
, pp. 14808-14813
-
-
Sarkar, C.A.1
Dodevski, I.2
Kenig, M.3
Dudli, S.4
Mohr, A.5
Hermans, E.6
Pluckthun, A.7
-
20
-
-
78651247312
-
Benchmarking membrane protein detergent stability for improving throughput of high-resolution X-ray structures
-
Sonoda Y., Newstead S., Hu N.J., Alguel Y., Nji E., Beis K., Yashiro S., Lee C., Leung J., Cameron A.D., et al. Benchmarking membrane protein detergent stability for improving throughput of high-resolution X-ray structures. Structure 2011, 19:17-25.
-
(2011)
Structure
, vol.19
, pp. 17-25
-
-
Sonoda, Y.1
Newstead, S.2
Hu, N.J.3
Alguel, Y.4
Nji, E.5
Beis, K.6
Yashiro, S.7
Lee, C.8
Leung, J.9
Cameron, A.D.10
-
21
-
-
69249146075
-
Engineering G protein-coupled receptors to facilitate their structure determination
-
Tate C.G., Schertler G.F. Engineering G protein-coupled receptors to facilitate their structure determination. Curr. Opin. Struct. Biol. 2009, 19:386-395.
-
(2009)
Curr. Opin. Struct. Biol.
, vol.19
, pp. 386-395
-
-
Tate, C.G.1
Schertler, G.F.2
|