Integration of cell-free protein coexpression with an enzyme-linked immunosorbent assay enables rapid analysis of protein-protein interactions directly from DNA

Protein Science

Volumn 20, Issue 8, 2011, Pages 1432-1438

  Layton, Curtis J ^a   Hellinga, Homme W ^a  

^a DUKE UNIVERSITY MEDICAL CENTER   (United States)

Author keywords

Binding assay; ELISA; Protein engineering; Protein protein interactions

Indexed keywords

ALANINE; ANKYRIN; DNA; MALTOSE BINDING PROTEIN; OFF7 PROTEIN; UNCLASSIFIED DRUG;

ADSORPTION; ANTIBODY AFFINITY; ARTICLE; CELL FREE SYSTEM; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; GENE MUTATION; IN VITRO STUDY; ISOTHERM; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN EXPRESSION; PROTEIN INTERACTION; PROTEIN PROTEIN INTERACTION; QUANTITATIVE ANALYSIS; SIGNAL TRANSDUCTION;

ADSORPTION; ANKYRIN REPEAT; BIOTECHNOLOGY; CELL-FREE SYSTEM; COMPUTER SIMULATION; DNA; ENZYME-LINKED IMMUNOSORBENT ASSAY; MALTOSE-BINDING PROTEINS; MODELS, CHEMICAL; PROTEIN ENGINEERING; PROTEIN INTERACTION DOMAINS AND MOTIFS; PROTEINS; RECOMBINANT PROTEINS;

EID: 79960665829     PISSN: 09618368     EISSN: 1469896X     Source Type: Journal    
DOI: 10.1002/pro.675     Document Type: Article

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