Quantitation of protein-protein interactions by thermal stability shift analysis

Protein Science

Volumn 20, Issue 8, 2011, Pages 1439-1450

  Layton, Curtis J ^a   Hellinga, Homme W ^a  

^a DUKE UNIVERSITY MEDICAL CENTER   (United States)

Author keywords

Binding assay; Protein engineering; Protein protein interactions; SYPRO Orange; Thermal stability shifts

Indexed keywords

ALANINE; ANKYRIN; FLUORESCENT DYE; MALTOSE BINDING PROTEIN; MUTANT PROTEIN; OFF7 PROTEIN; SYPRO ORANGE; UNCLASSIFIED DRUG;

ARTICLE; BINDING AFFINITY; GENE MUTATION; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN DENATURATION; PROTEIN PROTEIN INTERACTION; PROTEIN STABILITY; PROTEIN UNFOLDING; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; TEMPERATURE DEPENDENCE; THERMAL STABILITY SHIFT ANALYSIS; THERMODYNAMICS; THERMOSTABILITY;

ANKYRIN REPEAT; FLUORESCENT DYES; MALTOSE-BINDING PROTEINS; MODELS, CHEMICAL; MONTE CARLO METHOD; PROTEIN ENGINEERING; PROTEIN INTERACTION DOMAINS AND MOTIFS; PROTEIN STABILITY; PROTEIN UNFOLDING; PROTEINS; THERMODYNAMICS;

EID: 79960692993     PISSN: 09618368     EISSN: 1469896X     Source Type: Journal    
DOI: 10.1002/pro.674     Document Type: Article

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