Surface Marker Epithelial Cell Adhesion Molecule and E-cadherin Facilitate the Identification and Selection of Induced Pluripotent Stem Cells

Stem Cell Reviews and Reports

Volumn 7, Issue 3, 2011, Pages 722-735

  Chen, Hsin Fu ^a,b   Chuang, Ching Yu ^d   Lee, Wen Chih ^c,h   Huang, Hsiang Po ^e   Wu, Han Chung ^c   Ho, Hong Nerng ^a,b   Chen, Yu Ju ^f   Kuo, Hung Chih ^c,d,g,h  

^a NATIONAL TAIWAN UNIVERSITY   (Taiwan)

^b NATIONAL TAIWAN UNIVERSITY   (Taiwan)

^c INSTITUTE OF CELLULAR AND ORGANISMIC BIOLOGY   (Taiwan)

^d GENOMICS RESEARCH CENTER   (Taiwan)

^e NATIONAL TAIWAN UNIVERSITY HOSPITAL   (Taiwan)

^f INSTITUTE OF CHEMISTRY   (Taiwan)

^g TAIPEI MEDICAL UNIVERSITY   (Taiwan)

^h NATIONAL TAIWAN OCEAN UNIVERSITY   (Taiwan)

Author keywords

E cadherin; Embryonic stem cells; EpCAM; Induced pluripotent stem cells; Reprogramming

Indexed keywords

BIOLOGICAL MARKER; CADHERIN; CELL ADHESION MOLECULE; HOMEODOMAIN PROTEIN; NANOG PROTEIN, HUMAN; TUMOR ANTIGEN; TUMOR ASSOCIATED ANTIGEN GA733; TUMOR-ASSOCIATED ANTIGEN GA733;

ANIMAL; ARTICLE; CELL CULTURE; CELL SEPARATION; CELL STRAIN HEK293; CYTOLOGY; EXPERIMENTAL NEOPLASM; FIBROBLAST; FLOW CYTOMETRY; HUMAN; METABOLISM; METHODOLOGY; MOUSE; MOUSE MUTANT; NONOBESE DIABETIC MOUSE; NUCLEAR REPROGRAMMING; PLURIPOTENT STEM CELL; TRANSGENE;

ANIMALS; ANTIGENS, NEOPLASM; BIOLOGICAL MARKERS; CADHERINS; CELL ADHESION MOLECULES; CELL SEPARATION; CELLS, CULTURED; FIBROBLASTS; FLOW CYTOMETRY; HEK293 CELLS; HOMEODOMAIN PROTEINS; HUMANS; INDUCED PLURIPOTENT STEM CELLS; MICE; MICE, INBRED NOD; MICE, SCID; NEOPLASMS, EXPERIMENTAL; NUCLEAR REPROGRAMMING; TRANSGENES;

EID: 79960317924     PISSN: 15508943     EISSN: 15586804     Source Type: Journal    
DOI: 10.1007/s12015-011-9233-y     Document Type: Article

References (46)

1. Kao, C. F., Chuang, C. Y., Chen, C. H., & Kuo, H. C. (2008). Human pluripotent stem cells: current status and future perspectives. The Chinese Journal of Physiology, 51, 214-225.
2. Leeb, C., Jurga, M., McGuckin, C., Moriggl, R., & Kenner, L. (2010). Promising new sources for pluripotent stem cells. Stem Cell Reviews, 6, 15-26.
3. Takahashi, K., Tanabe, K., Ohnuki, M., et al. (2007). Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell, 131, 861-872.
4. Takahashi, K., & Yamanaka, S. (2006). Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell, 126, 663-676.
5. Yamanaka, S. (2009). A fresh look at iPS cells. Cell, 137, 13-17.
6. Wang, Y., Mah, N., Prigione, A., Wolfrum, K., Andrade-Navarro, M. A., & Adjaye, J. (2010). A transcriptional roadmap to the induction of pluripotency in somatic cells. Stem Cell Reviews, 6, 282-296.
7. Stadtfeld, M., Nagaya, M., Utikal, J., Weir, G., & Hochedlinger, K. (2008). Induced pluripotent stem cells generated without viral integration. Science, 322, 945-949.
8. Yu, J., Hu, K., Smuga-Otto, K., et al. (2009). Human induced pluripotent stem cells free of vector and transgene sequences. Science, 324, 797-801.
9. Meissner, A., Wernig, M., & Jaenisch, R. (2007). Direct reprogramming of genetically unmodified fibroblasts into pluripotent stem cells. Nature Biotechnology, 25, 1177-1181.
10. Okita, K., Ichisaka, T., & Yamanaka, S. (2007). Generation of germline-competent induced pluripotent stem cells. Nature, 448, 313-317.
11. Wernig, M., Meissner, A., Foreman, R., et al. (2007). In vitro reprogramming of fibroblasts into a pluripotent ES-cell-like state. Nature, 448, 318-324.
12. Tokuzawa, Y., Kaiho, E., Maruyama, M., et al. (2003). Fbx15 is a novel target of Oct3/4 but is dispensable for embryonic stem cell self-renewal and mouse development. Molecular and Cellular Biology, 23, 2699-2708.
13. Loh, Y. H., Wu, Q., Chew, J. L., et al. (2006). The Oct4 and Nanog transcription network regulates pluripotency in mouse embryonic stem cells. Nature Genetics, 38, 431-440.
14. Schmelzer, E., Zhang, L., Bruce, A., et al. (2007). Human hepatic stem cells from fetal and postnatal donors. The Journal of Experimental Medicine, 204, 1973-1987.
15. Stingl, J., Raouf, A., Emerman, J. T., & Eaves, C. J. (2005). Epithelial progenitors in the normal human mammary gland. Journal of Mammary Gland Biology and Neoplasia, 10, 49-59.
16. Went, P., Vasei, M., Bubendorf, L., et al. (2006). Frequent high-level expression of the immunotherapeutic target Ep-CAM in colon, stomach, prostate and lung cancers. British Journal of Cancer, 94, 128-135.
17. Schmelzer, E., Wauthier, E., & Reid, L. M. (2006). The phenotypes of pluripotent human hepatic progenitors. Stem Cells, 24, 1852-1858.
18. Dan, Y. Y., Riehle, K. J., Lazaro, C., et al. (2006). Isolation of multipotent progenitor cells from human fetal liver capable of differentiating into liver and mesenchymal lineages. Proceedings of the National Academy of Sciences of the United States of America, 103, 9912-9917.
19. Anderson, R., Schaible, K., Heasman, J., & Wylie, C. (1999). Expression of the homophilic adhesion molecule, Ep-CAM, in the mammalian germ line. Journal of Reproduction and Fertility, 116, 379-384.
20. Sundberg, M., Jansson, L., Ketolainen, J., et al. (2009). CD marker expression profiles of human embryonic stem cells and their neural derivatives, determined using flow-cytometric analysis, reveal a novel CD marker for exclusion of pluripotent stem cells. Stem Cell Research, 2, 113-124.
21. Kolle, G., Ho, M., Zhou, Q., et al. (2009). Identification of human embryonic stem cell surface markers by combined membrane-polysome translation state array analysis and immunotranscriptional profiling. Stem Cells, 27, 2446-2456.
22. Gonzalez, B., Denzel, S., Mack, B., Conrad, M., & Gires, O. (2009). EpCAM is involved in maintenance of the murine embryonic stem cell phenotype. Stem Cells, 27, 1782-1791.
23. Ng, V. Y., Ang, S. N., Chan, J. X., & Choo, A. B. (2010). Characterization of epithelial cell adhesion molecule as a surface marker on undifferentiated human embryonic stem cells. Stem Cells, 28, 29-35.
24. Lu, T. Y., Lu, R. M., Liao, M. Y., et al. (2010). Epithelial cell adhesion molecule regulation is associated with the maintenance of the undifferentiated phenotype of human embryonic stem cells. The Journal of Biological Chemistry, 285, 8719-8732.
25. Tang, A., Eller, M. S., Hara, M., Yaar, M., Hirohashi, S., & Gilchrest, B. A. (1994). E-cadherin is the major mediator of human melanocyte adhesion to keratinocytes in vitro. Journal of Cell Science, 107(Pt 4), 983-992.
26. Soncin, F., Mohamet, L., Eckardt, D., et al. (2009). Abrogation of E-cadherin-mediated cell-cell contact in mouse embryonic stem cells results in reversible LIF-independent self-renewal. Stem Cells, 27, 2069-2080.
27. Li, Z., Qiu, D., Sridharan, I., et al. (2010). Spatially resolved quantification of E-cadherin on target hES cells. The Journal of Physical Chemistry, 114, 2894-2900.
28. Li, L., Wang, S., Jezierski, A., et al. (2010). A unique interplay between Rap1 and E-cadherin in the endocytic pathway regulates self-renewal of human embryonic stem cells. Stem Cells, 28, 247-257.
29. Huang, H. P., Yu, C. Y., Chen, H. F., et al. (2010). Factors from human embryonic stem cell-derived fibroblast-like cells promote topology-dependent hepatic differentiation in primate embryonic and induced pluripotent stem cells. The Journal of Biological Chemistry, 285, 33510-33519.
30. Chen, H. F., Chuang, C. Y., Shieh, Y. K., Chang, H. W., Ho, H. N., & Kuo, H. C. (2009). Novel autogenic feeders derived from human embryonic stem cells (hESCs) support an undifferentiated status of hESCs in xeno-free culture conditions. Human Reproduction, 24, 1114-1125.
31. Chen, H. F., Kuo, H. C., Chien, C. L., et al. (2007). Derivation, characterization and differentiation of human embryonic stem cells: comparing serum-containing versus serum-free media and evidence of germ cell differentiation. Human Reproduction, 22, 567-577.
32. Cotterman, R., & Knoepfler, P. S. (2009). N-Myc regulates expression of pluripotency genes in neuroblastoma including lif, klf2, klf4, and lin28b. PLoS ONE, 4, e5799.
33. Malynn, B. A., de Alboran, I. M., O'Hagan, R. C., et al. (2000). N-myc can functionally replace c-myc in murine development, cellular growth, and differentiation. Genes & Development, 14, 1390-1399.
34. Blelloch, R., Venere, M., Yen, J., & Ramalho-Santos, M. (2007). Generation of induced pluripotent stem cells in the absence of drug selection. Cell Stem Cell, 1, 245-247.
35. Ruau, D., Ensenat-Waser, R., Dinger, T. C., et al. (2008). Pluripotency associated genes are reactivated by chromatin-modifying agents in neurosphere cells. Stem Cells, 26, 920-926.
36. Munz, M., Kieu, C., Mack, B., Schmitt, B., Zeidler, R., & Gires, O. (2004). The carcinoma-associated antigen EpCAM upregulates c-myc and induces cell proliferation. Oncogene, 23, 5748-5758.
37. Sridharan, R., Tchieu, J., Mason, M. J., et al. (2009). Role of the murine reprogramming factors in the induction of pluripotency. Cell, 136, 364-377.
38. Boyer, L. A., Lee, T. I., Cole, M. F., et al. (2005). Core transcriptional regulatory circuitry in human embryonic stem cells. Cell, 122, 947-956.
39. Brambrink, T., Foreman, R., Welstead, G. G., et al. (2008). Sequential expression of pluripotency markers during direct reprogramming of mouse somatic cells. Cell Stem Cell, 2, 151-159.
40. Hanna, J., Saha, K., Pando, B., et al. (2009). Direct cell reprogramming is a stochastic process amenable to acceleration. Nature, 462, 595-601.
41. Takeichi, M. (1990). Cadherins: a molecular family important in selective cell-cell adhesion. Annual Review of Biochemistry, 59, 237-252.
42. Takeichi, M. (1991). Cadherin cell adhesion receptors as a morphogenetic regulator. Science, 251, 1451-1455.
43. Rijsewijk, F., Schuermann, M., Wagenaar, E., Parren, P., Weigel, D., & Nusse, R. (1987). The Drosophila homolog of the mouse mammary oncogene int-1 is identical to the segment polarity gene wingless. Cell, 50, 649-657.
44. Ozawa, M., Baribault, H., & Kemler, R. (1989). The cytoplasmic domain of the cell adhesion molecule uvomorulin associates with three independent proteins structurally related in different species. The EMBO Journal, 8, 1711-1717.
45. Fukunaga, Y., Liu, H., Shimizu, M., Komiya, S., Kawasuji, M., & Nagafuchi, A. (2005). Defining the roles of beta-catenin and plakoglobin in cell-cell adhesion: isolation of beta-catenin/plakoglobin-deficient F9 cells. Cell Structure and Function, 30, 25-34.
46. Miyabayashi, T., Teo, J. L., Yamamoto, M., McMillan, M., Nguyen, C., & Kahn, M. (2007). Wnt/beta-catenin/CBP signaling maintains long-term murine embryonic stem cell pluripotency. Proceedings of the National Academy of Sciences of the United States of America, 104, 5668-5673.