Direct reprogramming of genetically unmodified fibroblasts into pluripotent stem cells

Nature Biotechnology

Volumn 25, Issue 10, 2007, Pages 1177-1181

  Meissner, Alexander ^a   Wernig, Marius ^a   Jaenisch, Rudolf ^a,b  

^a WHITEHEAD INSTITUTE FOR BIOMEDICAL RESEARCH   (United States)

^b MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

COMPUTER PROGRAMMING; FIBROBLASTS; MORPHOLOGY;

NEOMYCIN-RESISTANCE GENE; PLURIPOTENT CELLS; SOMATIC CELLS; STEM CELL-LIKE STATE;

GENETIC ENGINEERING;

NEOMYCIN; OCTAMER TRANSCRIPTION FACTOR 4;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; CELL CULTURE; CELL ISOLATION; CELL STRUCTURE; DONOR; EMBRYO; EXPERIMENT; FEMALE; FIBROBLAST; GENE INSERTION; GENETICS; MOUSE; NONHUMAN; PLURIPOTENT STEM CELL; PRIORITY JOURNAL; SOMATIC CELL;

ANIMALS; BIOTECHNOLOGY; CELL DIFFERENTIATION; CELL LINEAGE; CELLS, CULTURED; DNA-BINDING PROTEINS; FEMALE; FIBROBLASTS; KRUPPEL-LIKE TRANSCRIPTION FACTORS; MALE; MICE; OCTAMER TRANSCRIPTION FACTOR-3; PLURIPOTENT STEM CELLS; PROTO-ONCOGENE PROTEINS C-MYC; TRANS-ACTIVATORS; TRANSDUCTION, GENETIC;

IPS; MURINAE;

EID: 35148845584     PISSN: 10870156     EISSN: 15461696     Source Type: Journal    
DOI: 10.1038/nbt1335     Document Type: Article

References (19)

1. Takahashi, K. & Yamanaka, S. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell 126, 663-676 (2006).
2. Okita, K., Ichisaka, T. & Yamanaka, S. Generation of germline-competent induced pluripotent stem cells. Nature 448, 313-317 (2007).
3. Wernig, M. et al. In vitro reprogramming of fibroblasts into a pluripotent ES-cell-like state. Nature 448, 318-324 (2007).
4. Maherali, N. et al. Global epigenetic remodeling in directly reprogrammed fibroblasts. Cell Stem Cell 1, 55-70 (2007).
5. Cowan, C.A., Atienza, J., Melton, D.A. & Eggan, K. Nuclear reprogramming of somatic cells after fusion with human embryonic stem cells. Science 309, 1369-1373 (2005).
6. Tada, M., Takahama, Y., Abe, K., Nakatsuji, N. & Tada, T. Nuclear reprogramming of somatic cells by in vitro hybridization with ES cells. Curr. Biol. 11, 1553-1558 (2001).
7. Hochedlinger, K. & Jaenisch, R. Nuclear reprogramming and pluripotency. Nature 441, 1061-1067 (2006).
8. Yang, X. et al. Nuclear reprogramming of cloned embryos and its implications for therapeutic cloning. Nat. Genet. 39, 295-302 (2007).
9. Egli, D., Rosains, J., Birkhoff, G. & Eggan, K. Developmental reprogramming after chromosome transfer into mitotic mouse zygotes. Nature 447, 679-685 (2007).
10. Lengner, C. et al. Oct4 is dispensable for somatic stem cell self-renewal. Cell Stem Cell (in the press).
11. Yamanaka, S. Strategies and new developments in the generation of patient-specific pluripotent stem cells. Cell Stem Cell 1, 39-49 (2007).
12. Nagy, A. et al. Embryonic stem cells alone are able to support fetal development in the mouse. Development 110, 815-821 (1990).
13. Eggan, K. et al. Hybrid vigor, fetal overgrowth, and viability of mice derived by nuclear cloning and tetraploid embryo complementation. Proc. Natl. Acad. Sci. USA 98, 6209-6214 (2001).
14. Wang, Z. & Jaenisch, R. At most three ES cells contribute to the somatic lineages of chimeric mice and of mice produced by ES-tetraploid complementation. Dev. Biol. 275, 192-201 (2004).
15. Lerou, P.H. & Daley, G.Q. Therapeutic potential of embryonic stem cells. Blood Rev. 19, 321-331 (2005).
16. Sonntag, K.C., Simantov, R. & Isacson, O. Stem cells may reshape the prospect of Parkinson's disease therapy. Brain Res. Mol. Brain Res. 134, 34-51 (2005).
17. Scheffler, B., Edenhofer, F. & Brustle, O. Merging fields: stem cells in neurogenesis, transplantation, and disease modeling. Brain Pathol. 16, 155-168 (2006).
18. Naviaux, R.K., Costanzi, E., Haas, M. & Verma, I.M. The pCL vector system: rapid production of helper-free, high-titer, recombinant retroviruses. J. Virol. 70, 5701-5705 (1996).
19. Wernig, M. et al. Functional integration of embryonic stem cell-derived neurons in vivo. J. Neurosci. 24, 5258-5268 (2004).