Intestinal absorption of water-soluble vitamins in health and disease

Biochemical Journal

Volumn 437, Issue 3, 2011, Pages 357-372

  Said, Hamid M ^a,b  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b VA LONG BEACH HEALTHCARE SYSTEM   (United States)

Author keywords

Ascorbate; Biotin; Folate; Intestinal transport; Riboflavin; Thiamin

Indexed keywords

ASCORBIC ACID; BIOTIN; CARRIER PROTEIN; FOLIC ACID; GLUCOSE TRANSPORTER; HEPATOCYTE NUCLEAR FACTOR; KRUPPEL LIKE FACTOR; NICOTINIC ACID; PANTOTHENIC ACID; PROTON COUPLED FOLATE TRANSPORTER; PYRIDOXINE; REDUCED FOLATE CARRIER; REDUCED FOLATE TRANSPORTER 1; RIBOFLAVIN; THIAMIN TRANSPORTER; THIAMINE; UNCLASSIFIED DRUG; VITAMIN; WATER SOLUBLE VITAMIN;

ACTIVE TRANSPORT; ALCOHOL CONSUMPTION; CYTOLOGY; ENTEROPATHY; HOMEOSTASIS; HUMAN; INTESTINE ABSORPTION; INTESTINE EPITHELIUM CELL; INTESTINE FLORA; INTESTINE RESECTION; INTRACELLULAR SIGNALING; MEMBRANE TRANSPORT; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; REVIEW; TRANSCRIPTION REGULATION; VITAMIN DEFICIENCY;

ANIMALS; HUMANS; INTESTINAL ABSORPTION; SOLUBILITY; VITAMINS; WATER;

EID: 79960222400     PISSN: 02646021     EISSN: 14708728     Source Type: Journal    
DOI: 10.1042/BJ20110326     Document Type: Review

References (204)

1. Quadros, E. V. (2010) Advances in the understanding of cobalamin assimilation and metabolism. Br. J. Haematol. 148, 195-204
2. Fischer, H., Schwarzer, C. and Illek, B. (2004) Vitamin C controls the cystic fibrosis transmembrane conductance regulator chloride channel. Proc. Nat. Acad. Sci. U.S.A. 101, 3691-3696
3. Bianchi, J., Wilson, F. A. and Rose, R. C. (1986) Dehydroascorbic acid and ascorbic acid transport in the guinea pig ileum. Am. J. Physiol. 250, G461-G468
4. Choi, J. L. and Rose, R. C. (1989) Regeneration of ascorbic acid by rat colon. Proc. Soc. Exp. Biol. Med. 190, 369-374
5. Schell, D. A. and Bode, A. M. (1993) Measurement of ascorbic acid and dehydroascorbic acid in mammalian tissue utilizing HPLC and electrochemical detection. Biomed. Chromatogr. 7, 267-272
6. Wrong, O. M., Edmonds, C. J. and Chadwick, V. S. (1981) The Large Intestine: its Role in Mammalian Nutrition and Homeostasis, Wiley and Sons, New York
7. Rose, R. C. (1987) Intestinal absorption of water-soluble vitamins. In Physiology of the Gastrointestinal Tract (Johnson, L. R., ed.), pp. 1581-1596, Raven Press, New York
8. Said, H. M. (2004) Recent advances in carrier-mediated absorption of water-soluble vitamins. Annu. Rev. Physiol. 66, 419-446
9. Said, H. M. and Seetharam, B. (2006) Intestinal absorption of vitamins. In Physiology of the Gastrointestinal Tract, vol. 2, 4th edn (Johnson, L. R., Barrett, K. E., Ghishan, F. K., Merchand, J. M., Said, H. M. and Wood, J. D., eds), pp. 1791-1826, Academic Press, New York
10. +-dependent L-ascorbic acid transporters. Nature 399, 70-75
11. Wang, H., Mackenzie, B., Tsukaguchi, H., Weremowicz, S., Morton, C. C. and Hediger, M. A. (2000) Human vitamin C (L-ascorbic acid) transporter SVCT1. Biochim. Biophys. Res. Commun. 267, 488-494
12. + -dependent vitamin C transporter functionality. Biochem. Biophys. Res. Commun. 374, 123-127
13. Liang, W. J., Johnson, D. and Jarvis, S. M. (2001) Vitamin C transport systems of mammalian cells. Mol. Membr. Biol. 18, 87-95
14. Varma, S., Campbell, C. E. and Kuo, S. (2008) Functional role of conserved transmembrane segment 1 residues in human sodium-dependent vitamin C transporters. Biochemistry 47, 2952-2960 (Pubitemid 351328848)
15. Subramanian, V. S., Marchant, J. S., Boulware, M. J. and Said, H. M. (2004) A C-terminal region dictates the apical plasma membrane targeting of the human sodium-dependent vitamin C transporter-1 in polarized epithelia. J. Biol. Chem. 279, 27719-27728
16. Boyer, J. C., Campbell, C. E., Sigurdson, W. J. and Kuo, S. (2005) Polarized localization of vitamin C transports, SVCT1 and SVCT2, in epithelial cells. Biochem. Biophys. Res. Commun. 334, 150-156
17. Michels, A. J. and Hagen, T. M. (2009) Hepatocyte nuclear factor 1 is essential for transcription of sodium-dependent vitamin C transporter protein 1. Am. J. Physiol. Cell Physiol. 297, C1220-C1227
18. Reidling, J. C. and Rubin, S. A. (2011) Promoter analysis of the human ascorbic acid transporters SVCT1 and SVCT2: mechanism of adaptive regulation in liver epithelial cells. J. Nutr. Biochem. 22, 344-350
19. Karasov, W. H., Darken, B. W. and Bottum, M. C. (1991) Dietary regulation of intestinal ascorbate uptake in guinea pigs. Am. J. Physiol. 260, G108-G118
20. Rose, R. C. and Nahrwold, D. L. (1978) Intestinal ascorbic acid transport following diets of high or low ascorbic acid content. Int. J. Vitam. Nutr. Res. 48, 382-386
21. MacDonald, L., Thumser, A. E. and Sharp, P. (2002) Decreased expression of the vitamin C transporter SVCT1 by ascorbic acid in a human intestinal epithelial cell line. Br. J. Nutr. 87, 97-100
22. Amano, A., Aigaki, T., Maruyama, N. and Ishigami, A. (2010) Ascorbic acid depletion enhaces expression of the sodium-dependent vitamin C transporters, SVCT1 and SVCT2, and uptake of ascorbic acid in livers of SMP30/GNL knockout mice. Arch. Biochem. Biophys. 496, 38-44
23. Pinto, M., Leon, S. R., Tappay, M., Kedinger, M., Triadou, N., Dussayix, M., Lacvrix, B., Simon-Asswan, P., Haffer, N., Fiugh, J. and Zwiebaum, A. (1988) Enterocyte-like differentiation and polarization of the human colon carcinoma cell line Caco-2 in culture. Biol. Cell 47, 323-340
24. Maulén, N. P., Henriquez, E. A., Kempe, S., Cárcamo, J. G., Schmid-Kotsas, A., Bachem, M., Grünert, A., Bustamante, M. E., Nualart, F. and Vera, J. C. (2003) Up-regulation and polarized expression of the sodium-ascorbic acid transporter SVCT1 in post-confluent differentiated Caco-2 cells. J. Biol. Chem. 278, 9035-9041
25. Scheerger, S. B. and Zempleni, J. (2003) Expression of oncogenes depends on biotin in human small cell lung cancer cells NCI-H69. Int. J. Vit. Nutr. Res. 73, 461-467
26. Collins, J. C., Paietta, E., Green, R., Morell, A. G. and Stockert, R. J. (1988) Biotin-dependent expression of the asialoglycoprotein receptor in HepG2 cells. J. Biol. Chem. 263, 11280-11283
27. Vesely, D. L. (1982) Biotin enhances guanylate cyclase activity. Science 216, 1329-1330
28. Spence, J. T. and Koudelka, A. P. (1984) Effect of biotin upon the intracellular level of cGMP and the activity of glucokinase in cultured rat hepatocytes. J. Biol. Chem. 259, 6393-6396
29. Watanabe, T. (1983) Teratogenic effect of biotin deficiency in mice. J. Nutr. 113, 574-581
30. Cooper, W. A. and Brown, S. O. (1958) Tissue abnormalities in newborn rats from biotin-deficient mothers. Texas J. Sci. 10, 60-68
31. Mock, D. M., Mock, N. I., Stewart, C. W., LaBorde, J. B. and Hansen, D. K. (2003) Marginal biotin deficiency is teratogenic in ICR mice. J. Nutr. 133, 2519-2525
32. Zempleni, J. and Mock, D. M. (2000) Marginal biotin deficiency is teratogenic. Proc. Soc. Exp. Biol. Med. 223, 14-21
33. Sweetman, L. and Nyhan, W. L. (1986) Inheritable biotin-treatable disorders and associated phenomena. Annu. Rev. Nutr. 6, 314-343
34. Krause, K. H., Berlit, P. and Bonjour, J. P. (1982) Impaired biotin status in anticonvulsant therapy. Ann. Neurol. 12, 485-486
35. Lampen, J., Hahler, G. and Peterson, W. (1942) The occurrence of free and bound biotin. J. Nutr. 23, 11-21
36. Krause, K. H., Bonjour, J., Berlit, P. and Kochen, W. (1985) Biotin status of epileptics. Ann. N. Y. Acad. Sci. 447, 297-313
37. Forbes, G. M. and Forbes, A. (1997) Micronutrient status in patients receiving home parenteral nutrition. Nutrition 13, 941-944
38. Mock, D. M., deLorimer, A. A., Liebman, W. M., Sweetman, L. and Baker, H. (1981) Biotin deficiency: an unusual complication of parenteral alimentation. N. Engl. J. Med. 304, 820-823
39. Bonjour, J. P. (1980) Vitamins and alcoholism. V. Riboflavin, VI. Niacin, VII. Pantothenic acid, and VIII. Biotin. Int. J. Vitam. Nutr. Res. 50, 425-440
40. Mock, D. M., Stadler, D. D., Stratton, S. L. and Mock, N. I. (1997) Biotin status assessed longitudinally in pregnant women. J. Nutr. 127, 710-716
41. Banares, F. F., Lacruz, A. A., Gine, J. J., Esteve, M. and Gassull, M. A. (1989) Vitamin status in patients with inflammatory bowel disease. Am. J. Gastroenterol. 84, 744-748
42. Sorrell, M. F., Frank, O., Thomson, A. D., Aquino, A. and Baker, H. (1971) Absorption of vitamins from the large intestine. Nutr. Res. Int. 3, 143-148
43. Barth, C. A., Frigg, M. and Homogemeister, H. (1986) Biotin absorption from the hindgut of the pig. J. Anim. Physiol. Anim. Nutr. 55, 128-134
44. Brown, B. B. and Rosenberg, J. H. (1987) Biotin absorption by distal rat intestine. J. Nutr. 117, 2121-2126
45. Said, H. M., Ortiz, A., McCloud, E., Dyer, D., Moyer, M. P. and Rubin, S. (1998) Biotin uptake by human colonic epithelial NCM460 cells: a carrier-mediated process shared with pantothenic acid. Am. J. Physiol. 275, C1365-C1371
46. Wolf, B., Heard, G. S., Secor-McVoy, J. R. and Raetz, H. M. (1984) Biotinidase deficiency: the possible role of biotinidase in the processing of dietary protein-bound biotin. J. Inherit. Metab. Dis. 7, 121-122
47. Blanton, S. H., Pandya, A., Landa, B. L., Javaheri, R., Xia, X. J., Nance, W. E., Pomponio, R. J., Norrgard, K. J., Swango, K. L., Demirkol, M. et al. (2000) Fine mapping of the human biotinidase gene and haplotype analysis of five common mutations. Hum. Hered. 50, 102-111
48. Said, H. M., Ortiz, A., McCloud, E., Dyer, D., Moyer, M. P. and Rubin, S. (1998) Biotin uptake by the human colonic epithelial cells NCM460: a carrier-mediated process shared with pantothenic acid. Am. J. Physiol. 44, C1365-C1371
49. Said, H. M. (1999) Cellular uptake of biotin: mechanisms and regulation. J. Nutr. 129, 490S-493S
50. + gradient-dependent transport system for biotin in human intestinal brush border membrane vesicles. Am. J. Physiol. 253, G631-G636
51. Said, H. M. and Redha, R. (1988) Biotin transport in brush border membrane vesicles of rat small intestine. Biochim. Biophys. Acta 945, 195-201
52. Said, H. M., Nylander, W. and Redha, R. (1988) Biotin transport in human intestine: site of maximum transport and effect of pH. Gastroenterology 95, 1312-1317
53. Said, H. M. and Redha, R. (1988) Biotin transport in basolateral membrane vesicles of human intestine. Gastroenterology 94, 1157-1163
54. Said, H. M. (1991) Movement of biotin across the rat intestinal basolateral membrane: studies with membrane vesicles. Biochem. J. 279, 671-674
55. Subramanian, V. S., Marchant, J. S., Boulware, M. J., Ma, T. Y. and Said, H. M. (2009) Membrane targeting and intracellular trafficking of the human sodium-dependent multivitamin transporter in polarized epithelial cells. Am. J. Physiol. Cell Physiol. 296, C663-C671
56. Nabokina, S. M., Subramanian, V. S. and Said, H. M. (2003) Comparative analysis of ontogenic changes in renal and intestinal biotin transport in the rat. Am. J. Physiol. Renal Physiol. 284, F737-F742
57. Wang, H., Huang, W., Fei, Y-J., Xia, H., Yang-Feng, T. L., Leibach, F. H., Devoe, L. D., Ganapathy, V. and Prasad, P. D. (1999) Cloning, functional expression, gene structure, and chromosomal localization. J. Biol. Chem. 274, 14875-14883
58. +-dependent multivitamin transporter. Arch. Biochem. Biophys. 366, 95-106
59. Chatterjee, N. S., Kumar, C. K., Ortiz, A., Rubin, S. A. and Said, H. M. (1999) Molecular mechanism of the intestinal biotin transport process. Am. J. Physiol. 277, C605-C613
60. Balamurugan, K., Ortiz, A. and Said, H. M. (2003) Biotin uptake by human intestinal and liver epithelial cells: role of the SMVT system. Am. J. Physiol. Gastrointest. Liver Physiol. 285, G73-G77
61. Ghosal, A. and Said, H. M. (2011) Structure-function activity of the human sodium-dependent multivitamin transporter: role of His115 and His254. Am. J. Physiol. Cell Physiol. 300, C97-C104
62. Nabokina, S. M., Subramanian, V. S. and Said, H. M. (2010) Association of PDZ-containing protein PDZD11 with the human sodium-dependent multivitamin transporter. Am. J. Physiol. Gastrointest. Liver Physiol. 300, G561-G567
63. Chatterjee, N. S., Rubin, S. A. and Said, H. M. (2001) Molecular characterization of the 5′ regulatory region of rat sodium-dependent multivitamin transporter gene. Am. J. Physiol. Cell Physiol. 280, C548-C555
64. Dey, S., Subramanian, V. S., Chatterjee, N. S., Rubin, S. A. and Said, H. M. (2002) Characterization of the 5′ regulatory region of the human sodium-dependent multivitamin transporter, hSMVT. Biochim. Biophys. Acta 1574, 187-192
65. Reidling, J. C. and Said, H. M. (2007) Regulation of the human biotin transporter hSMVT promoter by KLF-4 and AP-2: confirmation of promoter activity in vivo. Am. J. Physiol. Cell Physiol 292, C1305-C1312
66. Said, H. M., Mock, D. M. and Collins, J. (1989) Regulation of intestinal biotin transport in the rat: effect of biotin deficiency and supplementation. Am. J. Physiol. 256, G306-G311
67. Reidling, J. C., Nabokina, S. M. and Said, H. M. (2007) Molecular mechanisms involved in the adaptive regulation of human intestinal biotin uptake: a study of the hSMVT system. Am. J. Physiol. Gastrointest. Liver Physiol. 292, G275-G281
68. Said, H. M. and Redha, R. (1988) Ontogenesis of the intestinal transport of biotin in the rat. Gastroenterology 94, 68-72 (Pubitemid 18029761)
69. Said, H. M., Ortiz, A., McCloud, E., Dyer, D., Moyer, M. P. and Rubin, S. A. (1998) Biotin uptake by the human colonic epithelial cells NCM460: A carrier-mediated process shared with pantothenic acid. Am. J. Physiol. 275, C1365-C1371
70. Said, H. M. (1999) Cellular uptake of biotin: mechanisms and regulation. J. Nutr. 129, 490S-493S
71. Fennelly, J., Frank, O., Baker, H. and Leevy, C. M. (1964) Peripheral neuropathy of the alcoholic: I, Aetiological role of aneurin and other B-complex vitamins. Br. Med. J. 2, 1290-1292
72. Subramanya, S. B., Subramanian, V. S., Kumar, J. S., Hoiness, R. and Said, H. M. (2011) Inhibition of intestinal biotin absorption by chronic alcohol feeding: cellular and molecular mechanisms. Am. J. Physiol. Gastrointest. Liver Physiol. 300, G494-G501
73. Subramanian, V. S., Subramanya, S. B. and Said, H. M. (2011) Chronic alcohol exposure negatively impacts the physiological and molecular parameters of the renal biotin reabsorption process. Am. J. Physiol. Renal Physiol. 300, F611-F617
74. Said, H. M., Redha, R. and Nylander, W. (1987) Biotin transport in the human intestine: inhibition by anticonvulsant drugs. Am. J. Clin. Nutr. 49, 127-131
75. Qiu, A., Jansen, M., Sakaris, A., Min, S. H., Chattopahyay, S., Tsai, E., Sandoval, C., Zhao, R., Akabas, M. H. and Goldman, I. D. (2006) Identification of an intestinal folate transporter and the molecular basis for hereditary folate malabsorption. Cell 127, 917-928
76. Geller, J., Kronn, D., Jayabose, S. and Sandoval, C. (2002) Hereditary folate malabsorption: family report and review of the literature. Medicine 81, 51-68 (Pubitemid 34081287)
77. Dudeja, P. K., Torania, S. A. and Said, H. M. (1997) Evidence for the existence of an electroneutral, pH-dependent, DIDS-sensitive carrier-mediated folate uptake mechanism in the human colonic luminal membrane vesicles. Am. J. Physiol. 272, G1408-G1415
78. Kumar, C. K., Moyer, M. P., Dudeja, P. K. and Said, H. M. (1997) A protein-tyrosine kinase regulated, pH-dependent carrier-mediated uptake system for folate by human normal colonic epithelial cell line NCM 460. J. Biol. Chem. 272, 6226-6231
79. Rong, N. I., Selhub, J., Goldin, B. R. and Rosenberg, I. (1991) Bacterially synthesized folate in rat large intestine is incorporated into host tissue folylpolyglutamates. J. Nutr. 121, 1955-1959
80. Zhao, R., Matherly, L. H. and Goldman, I. D. (2009) Membrane transporters and folate homeostasis: intestinal absorption and transport into systemic compartments and tissues. Expert Rev. Mol. Med. 11, 1-27
81. Sirotnak, F. M. and Tolner, B. (1999) Carrier-mediated membrane transport of folates in mammalian cells. Annu. Rev. Nutr. 19, 91-122
82. Dixon, K. H., Lampher, B. C., Chiu, J., Kelly, K. and Cowan, K. H. (1994) A novel cDNA restores reduced folate carrier activity and methotraxate sensitivity to transport deficient cells. J. Biol. Chem. 269, 17-20
83. Williams, F. M. R., Murray, R. C., Underhill, T. M. and Flintoff, W. F. (1994) Isolation of hamster cDNA clone coding for a function involved in methotrexate uptake. J. Biol. Chem. 269, 5810-5816
84. Nguyen, T. T., Dyer, D. L., Dunning, D. D., Rubin, S. A. and Said, H. M. (1997) Human intestinal folate transport: cloning, expression and distribution of complementary RNA. Gastroenterology 112, 783-791
85. Matherly, L. H. and Hou, Z. (2008) Structure and function of the reduced folate carrier a paradigm of a major facilitator superfamily mammalian nutrient transporter. Vitam. Horm. 79, 145-184
86. Wang, Y., Zhao, R., Russel, R. G. and Goldman, I. D. (2001) Localization of the murine reduced folate carrier as assessed by immunohistochemical analysis. Biochim. Biophys. Acta 1513, 49-54
87. Dudeja, P. K., Kode, A., Alnounou, M., Tyagi, S., Torania, S., Subramanian, V. S. and Said, H. M. (2001) Mechanism of folate transport across the human colonic basolateral membrane. Am. J. Physiol. Gastrointest. Liver Physiol. 281, G54-G60
88. Unal, E. S., Zhao, R., Qiu, A. and Coldman, D. (2008) N-linked glycosylation and its impact on the electrophoreticmobility and function of the human proton-coupled folate transporter (HsPCFT). Biochim. Biophys. Acta 1778, 1407-1414
89. Shayeghi, M., Latunde-Dada, G. O., Oakhill, J. S., Laftah, A. H., Takeuchi, K., Halliday, N., Khan, Y., Warley, A., McCann, F. E., Hider, R. C. et al. (2005) Identification of an intestinal heme transporter. Cell 122, 789-801 (Pubitemid 41242642)
90. Subramanian, V. S., Marchant, J. S. and Said, H. M. (2008) Apical membrane targeting and trafficking of the human proton-coupled folate transporter in polarized epithelia. Am. J. Physiol. Cell Physiol. 294, C233-C240
91. Said, H. M., Blair, J. A., Lucas, M. L. and Hilburn, M. E. (1986) Intestinal surface acid microclimate in vitro and in vivo in the rat. J. Lab. Clin. Med. 107, 420-424
92. Liu, X. Y., Witt, T. L. and Matherly, L. H. (2003) Restoration of high-level transport activity by human reduced folate carrier/ThTr1 thiamine transporter chimaeras: role of the transmembrane domain 6/7 linker region in reduced folate carrier function. Biochem. J. 369, 31-37
93. Sadlish, H., Williams, F. and Flintoff, W. (2002) Cytoplasmic domains of the reduced folate carrier are essential for trafficking, but not function. Biochem. J. 364, 777-786 (Pubitemid 34680854)
94. 281 residues in folate and proton translocation mediated by the human proton-coupled folate transporter SLC46A1. J. Biol. Chem. 284, 17846-17857
95. Subramanian, V. S., Marchant, J. S., Parker, I. and Said, H. M. (2001) Intracellular trafficking/membrane targeting of human reduced folate carrier expressed in Xenopus oocytes. Am. J. Physiol. Gastrointest. Liver Physiol. 281, G1477-G1486
96. Marchant, J. S., Subramanian, V. S., Parker, I. and Said, H. M. (2002) Intracellular trafficking and membrane targeting mechanisms of the human reduced folate carrier in mammalian epithelial cells. J. Biol. Chem. 277, 33325-33333
97. Ashokkumar, B., Nabokina, S. M., Ma, T. Y. and Said, H. M. (2009) Identification of dynein light chain road block-1 as a novel interaction partner with the human reduced folate carrier. Am. J. Physiol. Gastrointest. Liver Physiol. 297, G480-G487
98. Stark, M., Gonen, N. and Assaraf, Y. G. (2009) Functional elements in the minimal promoter of the human proton-coupled folate transporter. Biochem. Biophys. Res. Commun. 388, 79-85
99. Said, H. M., Chatterjee, H., Haq, R. U., Subramanian, V. S., Ortiz, A., Matherly, L. H., Sirotnak, F. M., Halsted, C. and Rubin, S. A. (2000) Adaptive regulation of intestinal folate uptake: effect of dietary folate deficiency. Am. J. Physiol. Cell Physiol. 279, C1889-C1895
100. Qiu,, A., Min, S. H., Jansen, M., Usha, M., Tsai, E., Cabelof, D. C., Matherly, L. H., Zhao, R., Akabas, M. H. and Goldman, I. D. (2007) Rodent intestinal folate transporters (SLC46A1): secondary structure functional properties and response to dietary folate restriction. Am. J. Physiol. Cell Physiol. 293, C1669-C1678
101. Subramanian, V. S., Chatterjee, N. and Said, H. M. (2003) Folate uptake in the human intestine: promoter activity and effect of folate deficiency. J. Cell. Physiol. 196, 403-408
102. Ashokkumar, B., Mohammed, Z. M., Vaziri, N. D. and Said, H. M. (2007) Effect of folate oversupplementation on folate uptake by human intestinal and renal epithelial cells. Am. J. Clin. Nutr. 86, 159-166
103. Shafizadeh, T. B. and Halsted, C. H. (2009) Postnatal ontogeny of intestinal GCPII and the RFC in pig. Am. J. Physiol. Gastrointest. Liver Physiol. 296, G476-G481
104. Said, H. M., Ghishan, F. K. and Murrell, J. E. (1985) Ontogenesis of intestinal transport of 5-methyltetrahydrofolate in the rat. Am. J. Physiol. 249, G567-G571
105. Balamurugan, K. and Said, H. M. (2003) Ontogenic regulation of folate transportacross rat jejunal brush-border membrane. Am. J. Physiol. Gastrointest. Liver Physiol. 285, G1068-G1073
106. Subramanian, V. S., Reidling, J. C. and Said, H. M. (2008) Differentiation-dependent regulation of the intestinal folate uptake process: studies with Caco-2 cells and native mouse intestine. Am. J. Physiol. Cell Physiol. 295, C828-C835
107. Said, H. M., Ma, T. Y., Ortiz, A., Tapia, A. and Valerio, C. K. (1997) Intracellular regulation of intestinal folate uptake: studies with cultured IEC-6 epithelial cells. Am. J. Physiol. 272, C729-C736
108. Halsted, C. H., Robles, E. A. and Mezey, E. (1971) Decreased jejunal uptake of labeled folic acid (3 H-PGA) in alcoholic patients: roles of alcohol and nutrition. N. Engl. J. Med. 285, 701-706
109. Villanueva, J. A., Devlin, A. M. and Halsted, C. H. (2001) Reduced folate carrier: tissue distribution and effects of chronic ethanol intake in the micropigAlcohol. Clin. Exp. Res. 25, 415-420
110. Hamid, A., Wani, N. A., Rana, S., Vaiphei, K., Mahmood, A. and Kaur, J. (2007) Down-regulation of reduced folate carrier may result in folate malabsorption across intestinal brush border membrane during experimental alcoholism. FEBS J. 274, 6317-6328 (Pubitemid 350215923)
111. Said, H. M., Mee, L., Sekar, V. T., Ashokkumar, B. and Pandol, S. J. (2010) Mechanism and regulation of folate uptake by pancreatic acinar cells: effect of chronic alcohol consumption. Am. J. Physiol. Gastrointest. Liver Physiol. 298, G985-G993
112. Halsted, C. H., Reisenauer, A. M., Romero, J. J., Cantor, D. S. and Ruebner, B. (1977) Jejunal perfusion of simple and conjugated folates in celiac sprue. J. Clin. Invest. 59, 933-940
113. Halsted, C. H., Reisenauer, A. M., Shane, B. and Tamura, T. (1978) Availability of monoglutamyl and polglutamyl folates in normal subjects and in patients with celiac sprue. Gut 19, 886-891 (Pubitemid 9067089)
114. Franklin, J. L. and Rosenberg, I. H. (1973) Impaired folic acid absorption in inflammatory bowel disease: effects of salicylazosulfapyridine (azulfidine). Gastroenterology 64, 517-525
115. Halsted, C. H., Gandhi, G. and Tamura, T. (1982) Folate levels inflammatory bowel disease. N. Engl. J. Med. 306, 1488
116. Urquhart, B. L., Gregor, J. C., Chande, N., Knauer, M. J., Tirona, R. G. and Kim, R. B. (2010) The human proton-coupled folate transporter (hPCFT): modulation of intestinal expression and function by drugs. Am. J. Physiol. Gastrointest. Liver Physiol. 298, G248-G254
117. Inoue, K., Nakai, Y., Ueda, S., Kamigaso, S., Ohta, K. Y., Hatakeyama, M., Hayashi, Y., Otagiri, M. and Yuasa, H. (2008) Functional characterization of PCFT/HCP1 as the molecular entity of the carrier-mediated intestinal folate transport system in the rat model. Am. J. Physiol. Gastrointest. Liver Physiol. 294, G660-G668
118. Jansen, G., Van der Heijden, J., Oerlemans, R., Lems, W. F., Ifergan, I., Scheper, R. J., Assaraf, Y. G. and Dijkmans, B. A. (2004) Sulfasalazine is a potent inhibitor of the reduced folate carrier: implications for combination therapies with methotrexate in rheumatoid arthritis. Arthritis Rheum. 50, 2130-2139
119. Fox, K. R., Adrian, C. and Hogben, M. (1974) Nicotinic acidactive transport by in vitro bullfrog small intestine. Biochim. Biophys. Acta 332, 336-340
120. Sadoogh-Abasian, F. and Evered, D. F. (1980) Absorption of nicotinic acid and nicotinamide from rat small intestine in vitro. Biochim. Biophys. Acta 598, 385-391
121. Simanjuntak, M. T., Tamai, I., Terasaki, T. and Tsuji, A. (1990) Carrier-mediated uptake of nicotinic acid by rat intestinal brush-border membrane vesicles and relation to monocarboxylic acid transport. J. Pharmacobiodyn. 13, 301-309 (Pubitemid 20286167)
122. Takanaga, H., Maeda, H., Yabuuchi, H., Tamai, I., Higashida, H. and Tsuji, A. (1996) Nicotinic acid transport mediated by pH-dependent anion antiporter and proton cotransporter in rabbit intestinal brush-border membrane. J. Pharm. Pharmacol. 48, 1073-1077
123. Guilarte, T. R. and Pravlik, K. (1983) Radiometric-microbiologic assay of niacin using Kloeckera brevis: analysis of human blood and food. J. Nutr. 113, 2587-2594 (Pubitemid 14222079)
124. Nabokina, S. M., Kashyap, M. L. and Said, H. M. (2005) Mechanism and regulation of human intestinal niacin uptake. Am. J. Physiol. Cell Physiol. 289, C97-C103
125. Said, H. M., Nabokina, S. M., Balamurugan, K., Hohammed, Z. M., Urbina, C. and Kashyap, M. L. (2007) Mechanism of nicotinic acid transport in human liver cells: experiments with HepG2 cells and primary hepatocytes. Am. J. Physiol. Cell Physiol. 293, C1773-C1778
126. Bahn, A., Hagos, Y., Reuter, S., Balen, D., Brzica, H., Krick, W., Burckhardt, B. C., Sabolic, I. and Burckhardt, G. (2008) Identification of a new urate and high affinity nicotinate transporter, hOAT10 (SLC22A13). J. Biol. Chem. 283, 16332-16341
127. Gopal, E., Fei, Y. J., Miyauchi, S., Zhuang, L., Prasad, P. D. and Ganapathy, V. (2005) Sodium-coupled and electrogenic transport of B-complex vitamin nicotinic acid by slc5a8, a member of the Na/glucose co-transporter gene family. Biochem. J. 388, 309-316 (Pubitemid 40732959)
128. Gopal, E., Miyauchi, S., Martin, P. M., Ananth, S., Roon, P., Smith, S. B. and Ganapathy, V. (2007) Transport of nicotinate and structurally related compounds by human SMCT1(SLC5A8) and its relevance to drug transport in the mammalian intestinal tract. Pharm. Res. 24, 575-584
129. Shibata, K., Gross, C. J. and Henderson, L. M. (1983) Hydrolysis and absorption of pantothenate and its coenzymes in the rat small intestine. J. Nutr. 113, 2107-2115
130. Gospe, S. M. (2002) Pyridoxine-dependent seizures: finding from recent studies pose new questions. Pediatr. Neurol. 26, 181-185
131. Linkswiler, H., Baumann, C. A. and Snell, E. E. (1951) Effect of aureomycin on the response of rats to various forms of vitamin B6. J. Nutr. 43, 565-573
132. Hamm, M. W., Hehansho, H. and Henderson, L. M. (1979) Transport and metabolism of pyridoxamine and pyridoxamine phosphate in the small intestine. J. Nutr. 109, 1552-1559
133. Middleton, H. M. (1979) Intestinal absorption of pyridoxal- 5′phosphate disappearance from perfused segments of rat jejunum in vivo. J. Nutr. 109, 975-981
134. Middleton, H. M. (1985) Uptake of pyridoxine by in vivo perfused segments of rat small intestine: a possible role for intracellular vitamin metabolism. J. Nutr. 115, 1079-1088
135. Yoshida, S., Hayashi, K. and Kawasaki, T. (1981) Pyridoxine transport in brush border membrane vesicles of guinea pig jejunum. J. Nutr. Sci. Vitaminol. 27, 311-317
136. Said, H. M., Ortiz, A. and Ma, T. Y. (2003) A carrier-mediated mechanism for pyridoxine uptake by human intestinal epithelial Caco-2 cells: regulation by a PKA-mediated pathway. Am. J. Physiol. Cell Physiol. 285, C1219-C1225
137. Said, Z. M., Subramanian, V. S., Vaziri, N. D. and Said, H. M. (2008) Pyridoxine uptake by colonocytes: a specific and regulated carrier-mediated process. Am. J. Physiol. Cell Physiol. 294, C1192-C1197
138. Wang, L.-D., Zhou, F.-Y., Li, X.-M., Sun, L.-D., Song, X., Jin, Y., Li, J.-M., Kong, G.-Q., Qi, H., Cui, J. et al. (2010) Genome-wide association study of esophageal squamous cell carcinoma in Chinese subjects identifies susceptibility loci at PLCE1 and C20orf54. Nat. Genet. 42, 759-763
139. Kasper, H. (1970) Vitamin absorption in the colon. Am. J. Proctol. 21, 341-345
140. Daniel, H., Binninger, E. and Rehner, G. (1983) Hydrolysis of FMN and FAD by alkaline phosphatase of the intestinal brush border membrane. Int. J. Vitam. Nutr. Res. 53, 109-114
141. Iinuma, S. (1995) Synthesis of riboflavin by intestinal bacteria. J. Vitam. 2, 6-13
142. Ocese, O., Pearson, P. B. and Schwiegert, B. S. (1948) The synthesis of certain B vitamins by the rabbit. J. Nutr. 35, 577-590
143. Yonezawa, A., Masuda, S., Katsura, T. and Inui, K. (2008) Identification and functional characterization of a novel human and rat riboflavin transporter, RFT1. Am. J. Physiol. Cell Physiol. 295, C632-C641
144. Yamamoto, S., Inoue, K., Ohta, K., Fukatsu, R., Maeda, J., Yoshida, Y. and Yuasa, H. (2009) Identification and functional characterization of rat riboflavin transporter 2. J. Biochem. 145, 437-443
145. Said, H. M. and Ma, T. Y. (1994) Mechanism of riboflavin uptake by Caco-2 human intestinal epithelial cells. Am. J. Physiol. 266, G15-G21
146. Said, H. M., Ortiz, A., Moyer, M. P. and Yanagawa, N. (2000) Riboflavin uptake by human-derived colonic epithelial NCM460 cells. Am. J. Physiol. Cell Physiol. 278, C270-C276
147. Yao, Y., Yonezawa, A., Yoshimatsu, H., Masuda, S., Katsura, T. and Inui, K.-I. (2010) Identification and comparative functional characterization of a new human riboflavin transporter hRFT3 expressed in the brain. J. Nutr. 140, 1220-1226
148. Green, P., Wiseman, M., Crow, Y. J., Houlden, H., Riphagen, S., Lin, J.-P., Raymon, F. L., Childs, A.-M., Sheridan, E., Edwards, S. and Josifova, D. J. (2010) Brown-Vialetto-Van Laere syndrome, a ponto-bulbar palsy with deafness, is caused by mutations in C20orf54 . Am. J. Hum. Genet. 86, 485-489
149. Johnson, J. O., Gibbs, J. R., Maldergem, L. V., Houlden, H. and Singleton, A. B. (2010) Exome sequencing in Brown-Vialetto-Van Laere syndrome. Am. J. Hum. Genet. 87, 567-570
150. Bosch, A. M., Abeling, N. G. G. M., IJ1st, L., Knoester, H., Van der Pol, W. L., Stroomer, A. E. M., Wanders, R. J., Visser, G., Wijburg, F. A., Duran, M. and Waterham, H. R. (2011) Brown-Vialetto-Van Laere and Fazio Londe syndrome is associated with a riboflavin transporter defect mimicking mild MADD: a new inborn error of metabolism with potential treatment. J. Inherit. Metab. Dis. 34, 159-164
151. Said, H. M. and Khani, R. (1993) Uptake of riboflavin across the brush border membrane of rat intestine: regulation by dietary vitamin levels. Gastroenterology 105, 1294-1298 (Pubitemid 23315301)
152. Said, H. M., Ghishan, F. K., Greene, H. L. and Hollander, D. (1985) Developmental maturation of riboflavin intestinal transport in the rat. Pediatr. Res. 19, 1175-1178
153. Said, H. M., Ma, T. Y. and Grant, K. (1994) Regulation of riboflavin intestinal uptake by protein kinase A: studies with Caco-2 cells. Am. J. Physiol. 267, G955-G959
154. Yanagawa, N., Shih, R. N., Jo, O. D. and Said, H. M. (2000) Riboflavin transport by isolated perfused rabbit renal proximal tubules. Am. J. Physiol. Cell Physiol. 279, C1782-C1786
155. Said, H. M. (2010) Thiamin. In Encyclopedia of Dietary Supplements (Coats, P. M., Betz, J. M., Blackman, M. R., Cragg, G. M., Levine, M., Moss, J., and White, J. D., eds), pp. 752-757, Informa HealthCare, New York
156. Tallaksen, C. M. E., Bohmer, T. and Bell, H. (1992) Blood and serum thiamin and thiamin phosphate esters concentrations in patients with alcohol dependence syndrome before and after thiamin treatment. Alcohol. Clin. Exp. Res. 16, 320-325
157. Leevy, C. M. and Baker, H. (1968) Vitamins and alcoholism. Am. J. Clin. Nutr. 21, 1325-1328
158. Tallaksen, C. M., Bell, H. and Bohmer, T. (1993) Thiamin and thiamin phosphate ester deficiency assessed by high performance liquid chromatography in four clinical cases of Werincke encephalopathy. Alcohol. Clin. Exp. Res. 17, 712-716
159. Saito, N., Kimura, M., Kuchiba, A. and Itokawa, Y. (1987) Blood thiamin levels in outpatients with diabetes mellitus. J. Nutr. Sci. Vitaminol. 33, 421-430
160. Thomson, A. D. (1966) The absorption of sulfur-labeled thiamin hydrochloride in control subjects and in patients with intestinal malabsorption. Clin. Sci. 31, 167-179
161. Seligmann, H., Halkin, H., Rauchfleisch, S., Kaufmann, N., Motro, M., Vered, Z. and Ezra, D. (1991) Thiamine deficiency in patients with congestive heart failure receiving long-term furosemide therapy: a pilot study. Am. J. Med. 91, 151-155
162. Diaz, G. A., Banikazemai, M., Oishi, K., Desnick, R. J. and Gelb, B. D. (1999) Mutations in a new gene encoding a thiamin transporter cause thiamin-responsive megaloblastic anaemia syndrome. Nat. Genet. 22, 309-312
163. Labay, V., Raz, T., Baron, D., Mandel, H., Williams, H., Barrett, T., Szargel, R., McDonald, L., Shalata, A., Nosaka, K. et al. (1999) Mutations in SLC19A2 cause thiamin-responsive megaloblastic anaemia associated with diabetes mellitus and deafness. Nat. Genet. 22, 300-304
164. Fleming, J. C., Tartaglini, E., Steinkamp, M. P., Schorderet, D. F., Cohen, N. and Neufeld, E. J. (1999) The gene mutated in thiamine-response anaemia with diabetes and deafness (TRMA) encodes a functional thiamine transporter. Nat. Genet. 22, 305-308
165. Mandel, H., Berant, M., Hazani, A. and Naveh, Y. (1984) Thiamine-dependent beriberi in the thiamine-responsive anemia syndrome. N. Engl. J. Med. 311, 836-838
166. Rindi, G., Patrini, C., Laforenza, U., Mandel, H., Berant, M., Viana, M. B., Poggi, V. and Zarra, A. N. (1994) Further studies on erythocyte thiamin transport and phosphorylation in seven patients with thiamin-responsive megaloblastic anaemia. J. Inherit. Metab. Dis. 17, 667-677
167. Kono, S., Miyajima, H., Yoshida, K., Togawa, A., Shirakawa, K. and Suzuki, H. (2009) Mutations in a thiamine-transporter gene and Wernicke's-like encephalopathy. N. Engl. J. Med. 360, 1792-1794
168. Sklan, D. and Trostler, N. (1977) Site and extend of thiamin absorption in the rat. J. Nutr. 107, 353-356 (Pubitemid 8071938)
169. Guerrant, N. B. and Dutcher, R. A. (1932) The assay of vitamins B and G as influenced by coprophagy. J. Biol. Chem. 98, 225-235
170. Guerrant, N. B., Dutcher, R. A. and Brown, R. A. (1937) Further studies concerning formation of B vitamins in digestive tract of rat. J. Nutr. 13, 305-315
171. Najjar, V. A. and Holt, L. E. (1943) The biosynthesis of thiamin in man and its implication in human nutrition. JAMA, J. Am. Med. Assoc. 123, 683-684
172. Rindi, G. and Laforenza, U. (2000) Thiamin intestinal transport and related issues: recent aspects. Proc. Soc. Exp. Biol. Med. 224, 246-255
173. Dudeja, P. K., Tyagi, S., Kavilaveettil, R. J., Gill, R. and Said, H. M. (2001) Mechanism of thiamin uptake by human jejunal brush-border membrane vesicles. Am. J. Physiol. Cell Physiol. 281, C786-C792
174. Dudeja, P. K., Tyagi, S., Gill, R. and Said, H. M. (2003) Evidence for carrier-mediated mechanism for thiamin transport to human jejunal basolateral membrane vesicles. Digest. Dis. Sci. 48, 109-115
175. Rajgopal, A., Edmondson, A., Goldman, D. and Zhao, R. (2001) SLC19A3 encodes a second thiamin transporter ThTr2. Biochim. Biophys. Acta 1537, 175-178
176. Eudy, J. D., Spiegelstein, O., Baber, R. C., Wlodarczyk, B. J., Talbot, J. and Finnell, R. H. (2000) Identification and characterization of the human and mouse SLC19A3 gene: a novel member of the reduced folate family of micronutrient transporter genes. Mol. Gen. Metabol. 71, 581-590
177. Subramanian, V. S., Marchant, J. S., Parker, I. and Said, H. M. (2003) Cell biology of the human thiamin transporter-1 (hTHTR1): intracellular trafficking and membrane targeting mechanisms. J. Biol. Chem. 278, 3976-3984
178. Said, H. M., Balamurugan, K., Subramanian, V. S. and Marchant, J. S. (2003) Expression and functional contribution of hTHTR-2 in thiamin absorption in human intestine. Am. J. Physiol. Gastrointest. Liver Physiol. 286, G491-G498
179. Reidling, J. C., Lambrecht, N., Kassir, M. and Said, H. M. (2010) Impaired intestinal vitamin B1 (thiamin) uptake in thiamin transporter-2- deficient mice. Gastroenterology 138, 1802-1809
180. Neufeld, E. J., Fleming, J. C., Tyartaglini, E. and Steinkamp, M. P. (2001) Thiamine-responsive megaloblastic anemia syndrome: a disorder of high-affinity thiamine transport. Blood Cells Mol. Dis. 27, 135-138
181. Lagarde, W. H., Underwood, L. E., Moats-Staats, B. M. and Calikoglu, A. S. (2004) Novel mutation in the SLC19A2 gene in an African-American female with thiamine-responsive megaloblastic anemia syndrome. Am. J. Med. Genet. A 125A, 299-305 (Pubitemid 38294851)
182. Subramanian, V. S., Marchant, J. S. and Said, H. M. (2007) Targeting and intracellular trafficking of clinically relevant hTHTR1 mutations in human cell lines. Clin. Sci. 113, 93-102
183. Balamurugan, K. and Said, H. M. (2002) Functional role of specific amino acid residues in human thiamin transporter SLC19A2: mutational analysis. Am. J. Physiol. Gastrointest Liver Physiol. 283, G37-G43
184. Zeng, W. Q., Al-Yamani, E., Acierno, Jr., J. S., Slaugenhaupt, S., Gillis, T., MacDonald, M. E., Ozand, P. T. and Gusella, J. F. (2005) Biotin-responsive basal ganglia disease maps to 2q36.3 and is due to mutations in SLC19A3 . Am. J. Hum. Genet. 77, 16-26
185. Subramanian, V. S., Marchant, J. S. and Said, H. M. (2006) Biotin-responsive basal ganglia disease-linked mutations inhibit thiamine transport via hTHTR2: biotin is not a substrate for hTHTR2. Am. J. Physiol. Cell Physiol. 291, C851-C859
186. Subramanian, V. S., Marchant, J. S. and Said, H. M. (2006) Targeting and trafficking of the human thiamine transporter-2 in epithelial cells. J. Biol. Chem. 281, 5233-5245
187. Reidling, J. C., Subramanian, V. S., Dudeja, P. K. and Said, H. M. (2002) Expression and promoter analysis of SLC19A2 in the human intestine. Biochim. Biophys. Acta 1561, 180-187
188. Reidling, J. C. and Said, H. M. (2003) In vitro and in vivo characterization of the minimal promoter region of the human thiamin transporter SLC19A2 . Am. J. Physiol. Cell Physiol. 285, C633-C641
189. Nabokina, S. and Said, H. M. (2004) Characterization of the 5′-regulatory region of the human thiamin transporter SLC19A3: in vitro and in vivo studies. Am. J. Physiol. Gastrointest. Liver Physiol 287, G822-G829
190. Laforenza, U., Patrini, C., Alvisi, C., Faelli, A., Licandro, A. and Rindi, G. (1997) Thiamin uptake in human intestinal biopsy specimens, including observations from a patient with acute thiamin deficiency. Am. J. Clin. Nutr. 66, 320-326
191. Reidling, J. C. and Said, H. M. (2005) Adaptive regulation of intestinal thiamin uptake: molecular mechanism using wild-type and transgenic mice carrying hTHTR-1 and -2 promoters. Am. J. Physiol. Gastrointest. Liver Physiol. 288, G1127-G1134
192. Reidling, J. C., Nabokina, S. M., Balamurugan, K. and Said, H. M. (2006) Developmental maturation of intestinal and renal thiamin uptake: studies in wild-type and transgenic mice carrying human THTR-1 and 2 promoters. J. Cell. Physiol. 206, 371-377
193. Nabokina, S. M., Reidling, J. C. and Said, H. M. (2005) Differentiation-dependent up-regulation of intestinal thiamin uptake: cellular and molecular mechanisms. J. Biol. Chem. 280, 32676-32682
194. Said, H. M., Ortiz, A., Kumar, C. K., Chatterjee, N., Dudeja, P. K. and Rubin, S. A. (1999) Transport of thiamin in the human intestine: mechanism and regulation in intestinal epithelial cell model Caco-2. Am. J. Physiol. 277, C645-C651
195. Said, H. M., Ortiz, A., Subramanian, V. S., Neufeld, E. J., Moyer, M. P. and Dudeja, P. K. (2001) Mechanism of thiamin uptake by human colonocytes: studies with cultured colonic epithelial cell line NCM460. Am. J. Physiol. Gastrointest. Liver Physiol. 281, G144-G150
196. Ashokkumar, B., Vaziri, N. D. and Said, H. M. (2006) Thiamin uptake by the human-derived renal epithelial (HEK-293) cells: cellular and molecular mechanisms. Am. J. Physiol. Renal Physiol. 291, F796-F805
197. Subramanian, V. S., Mohammed, Z. M., Molina, A., Marchant, J. S., Vaziri, N. D. and Said, H. M. (2007) Vitamin B1 (thiamine) uptake by human retinal pigment epithelial (ARPE-19) cells: mechanism and regulation. J. Physiol. 582, 73-85
198. Mee, L., Nabokina, S. M., Sekar, V. T., Subramanian, V. S., Maedler, K. and Said, H. M. (2009) Pancreatic beta cells and islets take up thiamin by a regulated carrier-mediated process: studies using mice and human pancreatic preparations. Am. J. Physiol. Gastrointest. Liver Physiol. 297, G197-G206
199. Hoyumpa, Jr, A. M. (1980) Mechanisms of thiamin deficiency in chronic alcoholism. Am. J. Clin. Nutr. 33, 2750-2761
200. Gastaldi, G., Casirola, D., Ferrari, G. and Rindi, G. (1989) Effect of chronic ethanol administration on thiamin transport in microvillous vesicles of rat small intestine. Alcohol Alcohol. 24, 83-89 (Pubitemid 19100963)
201. Subramanya, S. B., Subramanian, V. S. and Said, H. M. (2010) Chronic alcohol consumption and intestinal absorption: effects on physiological and molecular parameters of the uptake process. Am. J. Physiol. Gastrointest. Liver Physiol. 299, G23-G31
202. Subramanian, V. S., Subramanya, S. B., Tsukamoto, H. and Said, H. M. (2010) Effect of chronic alcohol feeding on physiological and molecular parameters of renal thiamin transport. Am. J. Physiol. Renal Physiol. 299, F28-F34
203. Ashokkumar, B., Kumar, J. S., Hecht, G. A. and Said, H. M. (2009) Enteropathogenic Escherichia coli inhibits intestinal vitamin B1 (thiamin) uptake: studies with human-derived intestinal epithelial Caco-2 cells. Am. J. Physiol. Gastrointest. Liver Physiol. 297, G825-G833
204. Bukhari, F. J., Moradi, H., Gollapudi, P., Kim, H. J., Vaziri, N. D. and Said, H. M. (2010) Effect of chronic kidney disease on the expression of thiamin and folic acid transporters. Nephrol. Dial. Transplant. 26, 2137-2144