Real time analysis of β2-adrenoceptor-mediated signaling kinetics in Human Primary Airway Smooth Muscle Cells reveals both ligand and dose dependent differences

Respiratory Research

Volumn 12, Issue 1, 2011, Pages

  Billington, Charlotte K ^a   Hall, Ian P ^a  

^a UNIVERSITY OF NOTTINGHAM   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

BETA 2 ADRENERGIC RECEPTOR; CYCLIC AMP; FORMOTEROL; INDACATEROL; ISOPRENALINE; SALBUTAMOL; SALMETEROL; ADRB2 PROTEIN, HUMAN; BETA 2 ADRENERGIC RECEPTOR STIMULATING AGENT; BRONCHODILATING AGENT; HYBRID PROTEIN; LIGAND;

AIRWAY SMOOTH MUSCLE CELL; ARTICLE; BRONCHODILATATION; CELL SURFACE; CHRONIC OBSTRUCTIVE LUNG DISEASE; CONCENTRATION RESPONSE; CONFOCAL MICROSCOPY; CONTROLLED STUDY; FLUORESCENCE RESONANCE ENERGY TRANSFER; HUMAN; HUMAN CELL; ISOTOPE LABELING; LIPOPHILICITY; NEGATIVE FEEDBACK; SIGNAL TRANSDUCTION; BIOSYNTHESIS; BRONCHUS; CELL CULTURE; COMPARATIVE STUDY; DOSE RESPONSE; DRUG EFFECT; GENETIC PROCEDURES; GENETIC TRANSFECTION; KINETICS; METABOLISM; REPORTER GENE; SMOOTH MUSCLE FIBER;

ADRENERGIC BETA-2 RECEPTOR AGONISTS; BIOSENSING TECHNIQUES; BRONCHI; BRONCHODILATOR AGENTS; CELLS, CULTURED; CYCLIC AMP; DOSE-RESPONSE RELATIONSHIP, DRUG; FLUORESCENCE RESONANCE ENERGY TRANSFER; GENES, REPORTER; HUMANS; KINETICS; LIGANDS; MICROSCOPY, CONFOCAL; MYOCYTES, SMOOTH MUSCLE; RECEPTORS, ADRENERGIC, BETA-2; RECOMBINANT FUSION PROTEINS; SIGNAL TRANSDUCTION; TRANSFECTION;

EID: 79959765175     PISSN: 14659921     EISSN: 1465993X     Source Type: Journal    
DOI: 10.1186/1465-9921-12-89     Document Type: Article

References (31)

1. Hall IP TA: Beta-agonists. In Asthma. 3rd edition. Edited by: Clark TH GS, Lee TH. London: Chapman and Hall; 1992:341-365.
2. Sears MR, Lotvall J: Past, present and future-beta2-adrenoceptor agonists in asthma management. Respir Med 2005,99(2):152-170.
3. Cazzola M, Matera MG: Novel long-acting bronchodilators for COPD and asthma. Br J Pharmacol 2008,155(3):291-299.
4. Billington CK, Penn RB: Signaling and regulation of G protein-coupled receptors in airway smooth muscle. Respir Res 2003,4(1):2.
5. Roscioni SS, Kistemaker LE, Menzen MH, Elzinga CR, Gosens R, Halayko AJ, Meurs H, Schmidt M: PKA and Epac cooperate to augment bradykinin-induced interleukin-8 release from human airway smooth muscle cells. Respir Res 2009, 10:88.
6. Roscioni SS, Maarsingh H, Elzinga CR, Schuur J, Menzen M, Halayko AJ, Meurs H, Schmidt M: Epac as a novel effector of airway smooth muscle relaxation. J Cell Mol Med 2010.
7. Roscioni SS, Prins AG, Elzinga CR, Menzen MH, Dekkers BG, Halayko AJ, Meurs H, Maarsingh H, Schmidt M: Functional roles of Epac and PKA in human airway smooth muscle phenotype plasticity. Br J Pharmacol 2011, in press.
8. Yan H, Deshpande DA, Misior AM, Miles MC, Saxena H, Riemer EC, Pascual RM, Panettieri RA, Penn RB: Anti-mitogenic effects of beta-agonists and PGE2 on airway smooth muscle are PKA dependent. FASEB J 2011,25(1):389-397.
9. Kassel KM, Wyatt TA, Panettieri RA Jr., Toews ML: Inhibition of human airway smooth muscle cell proliferation by beta 2-adrenergic receptors and cAMP is PKA independent: evidence for EPAC involvement. Am J Physiol Lung Cell Mol Physiol 2008,294(1):L131-138.
10. van der Krogt GN, Ogink J, Ponsioen B, Jalink K: A comparison of donor-acceptor pairs for genetically encoded FRET sensors: application to the Epac cAMP sensor as an example. PLoS ONE 2008,3(4):e1916.
11. Ponsioen B, Zhao J, Riedl J, Zwartkruis F, van der Krogt G, Zaccolo M, Moolenaar WH, Bos JL, Jalink K: Detecting cAMP-induced Epac activation by fluorescence resonance energy transfer: Epac as a novel cAMP indicator. EMBO Rep 2004,5(12):1176-1180.
12. Jalink KvR, J: FilterFRET:Quantitative imaging of sensitized emission. In FRET and FLIM Techniques. Volume 33. Edited by: Gadella TWJ. Burlington: Elsevier; 2009:289-349.
13. Le Jeune IR, Shepherd M, Van Heeke G, Houslay MD, Hall IP: Cyclic AMP-dependent transcriptional up-regulation of phosphodiesterase 4D5 in human airway smooth muscle cells. Identification and characterization of a novel PDE4D5 promoter. J Biol Chem 2002,277(39):35980-35989.
14. Peel SE, Liu B, Hall IP: A key role for STIM1 in store operated calcium channel activation in airway smooth muscle. Respir Res 2006, 7:119.
15. Hall IP, Kotlikoff M: Use of cultured airway myocytes for study of airway smooth muscle. Am J Physiol 1995,268(1 Pt 1):L1-11.
16. Hall IP, Widdop S, Townsend P, Daykin K: Control of cyclic AMP levels in primary cultures of human tracheal smooth muscle cells. Br J Pharmacol 1992,107(2):422-428.
17. Billington CK, Le Jeune IR, Young KW, Hall IP: A major functional role for phosphodiesterase 4D5 in human airway smooth muscle cells. Am J Respir Cell Mol Biol 2008,38(1):1-7.
18. Sayers I, Hawley J, Stewart CE, Billington CK, Henry A, Leighton-Davies JR, Charlton SJ, Hall IP: Pharmacogenetic characterization of indacaterol, a novel beta(2)-adrenoceptor agonist. Br J Pharmacol 2009,158(1):277-86.
19. Zaccolo M, Pozzan T: Discrete microdomains with high concentration of cAMP in stimulated rat neonatal cardiac myocytes. Science 2002,295(5560):1711-1715.
20. van Rheenen J, Langeslag M, Jalink K: Correcting confocal acquisition to optimize imaging of fluorescence resonance energy transfer by sensitized emission. Biophys J 2004,86(4):2517-2529.
21. Woo AY, Wang TB, Zeng X, Zhu W, Abernethy DR, Wainer IW, Xiao RP: Stereochemistry of an agonist determines coupling preference of beta2-adrenoceptor to different G proteins in cardiomyocytes. Mol Pharmacol 2009,75(1):158-165.
22. Evans BA, Sato M, Sarwar M, Hutchinson DS, Summers RJ: Ligand-directed signalling at beta-adrenoceptors. British Journal of Pharmacology 2010,159(5):1022-1038.
23. Hoffmann C, Zurn A, Bunemann M, Lohse MJ: Conformational changes in G-protein-coupled receptors-the quest for functionally selective conformations is open. Br J Pharmacol 2008,153(Suppl 1):S358-366.
24. Kobilka BK, Deupi X: Conformational complexity of G-protein-coupled receptors. Trends Pharmacol Sci 2007,28(8):397-406.
25. Vaidehi N, Kenakin T: The role of conformational ensembles of seven transmembrane receptors in functional selectivity. Curr Opin Pharmacol 2010,10(6):775-81.
26. Urban JD, Clarke WP, von Zastrow M, Nichols DE, Kobilka B, Weinstein H, Javitch JA, Roth BL, Christopoulos A, Sexton PM, et al.: Functional selectivity and classical concepts of quantitative pharmacology. J Pharmacol Exp Ther 2007,320(1):1-13.
27. Walker JK, Penn RB, Hanania NA, Dickey BF, Bond RA: New perspectives regarding beta(2) -adrenoceptor ligands in the treatment of asthma. Br J Pharmacol 163(1):18-28.
28. Rosethorne EM, Turner RJ, Fairhurst RA, Charlton SJ: Efficacy is a contributing factor to the clinical onset of bronchodilation of inhaled beta(2)-adrenoceptor agonists. Naunyn Schmiedebergs Arch Pharmacol 2010,382(3):255-263.
29. Reiner S, Ambrosio M, Hoffmann C, Lohse MJ: Differential signaling of the endogenous agonists at the β 2-adrenergic receptor. J Biol Chem 2010,285(46):36188-98.
30. Nikolaev VO, Hoffmann C, Bunemann M, Lohse MJ, Vilardaga JP: Molecular basis of partial agonism at the neurotransmitter alpha2A-adrenergic receptor and Gi-protein heterotrimer. J Biol Chem 2006,281(34):24506-24511.
31. Schroder R, Janssen N, Schmidt J, Kebig A, Merten N, Hennen S, Muller A, Blattermann S, Mohr-Andra M, Zahn S, et al.: Deconvolution of complex G protein-coupled receptor signaling in live cells using dynamic mass redistribution measurements. Nature Biotechnology 2010,28(9):943-950.