Identifying protein construct variants with increased crystallization propensity - A case study

Protein Science

Volumn 15, Issue 12, 2006, Pages 2718-2728

  Malawski, Guido A ^a   Hillig, Roman C ^a   Monteclaro, Felipe ^b   Eberspaecher, Uwe ^a   Schmitz, Arndt A P ^a   Crusius, Kerstin ^a   Huber, Martina ^a   Egner, Ursula ^a   Donner, Peter ^a   Müller Tiemann, Beate ^a  

^a BAYER PHARMA AG   (Germany)

^b BAYER AG   (Germany)

Author keywords

Chromatography; Construct design; Expression screening; High throughput melting point analysis; High throughput protein production; Protein characterization; Protein crystallization; Protein isolation

Indexed keywords

MITOGEN ACTIVATED PROTEIN KINASE 1;

ARTICLE; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; CRYSTALLIZATION; MELTING POINT; MOLECULAR CLONING; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN VARIANT; THERMOSTABILITY; X RAY CRYSTALLOGRAPHY;

CLONING, MOLECULAR; CRYSTALLIZATION; ENZYME STABILITY; HUMANS; MODELS, MOLECULAR; MUTANT PROTEINS; PROTEIN CONFORMATION; PROTEIN DENATURATION; PROTEIN KINASES; RECOMBINANT PROTEINS; TEMPERATURE; VARIATION (GENETICS);

EID: 33751542139     PISSN: 09618368     EISSN: 1469896X     Source Type: Journal    
DOI: 10.1110/ps.062491906     Document Type: Article

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