An intein with genetically selectable markers provides a new approach to internally label proteins with GFP

BMC Biotechnology

Volumn 11, Issue , 2011, Pages

  Ramsden, Richard ^a   Arms, Luther ^a   Davis, Trisha N ^a   Muller, Eric G D ^a  

^a UNIVERSITY OF WASHINGTON SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMINOGLYCOSIDES; BIOCHEMICAL ACTIVITY; DNA ENCODING; E. COLI; ENDONUCLEASES; EXTEINS; FOLDED STRUCTURES; GENETIC BACKGROUNDS; GENETIC MARKERS; GENETIC SELECTION; HIGH EFFICIENCY; HYGROMYCIN; HYGROMYCIN RESISTANCE; IN-VIVO; INTEINS; N-TERMINALS; NUCLEASE ACTIVITY; PENICILLIUM CHRYSOGENUM; PHOSPHOTRANSFERASES; PRECURSOR PROTEINS; PROTEIN SEQUENCES; PROTOTROPY; S.CEREVISIAE; SELECTABLE MARKERS; TARGET PROTEINS; TRANSCRIPTIONAL ACTIVATORS;

AMINO ACIDS; EFFICIENCY; ESCHERICHIA COLI; GENE ENCODING; MOLECULAR BIOLOGY; POLYMERASE CHAIN REACTION; YEAST;

CALMODULIN;

AMINOGLYCOSIDE; ANTIBIOTIC G 418; CALMODULIN; ENDONUCLEASE; EXTEIN; GREEN FLUORESCENT PROTEIN; HISTIDINE; HYGROMYCIN; HYGROMYCIN B; IMIDAZOLE DERIVATIVE; INTEIN; LEUCINE; LEXA PROTEIN; PHOSPHOTRANSFERASE; PROTEIN VP16; CMD1 PROTEIN, S CEREVISIAE; HYBRID PROTEIN; HYDROLYASE; HYGROMYCIN B KINASE; HYGROMYCIN-B KINASE; IMIDAZOLEGLYCEROLPHOSPHATE DEHYDRATASE; KANAMYCIN KINASE; OLIGOPEPTIDE; SACCHAROMYCES CEREVISIAE PROTEIN; SCHIZOSACCHAROMYCES POMBE PROTEIN;

AMINO TERMINAL SEQUENCE; ANTIBIOTIC RESISTANCE; ARTICLE; BIOCHEMISTRY; CHEMICAL LABELING; ESCHERICHIA COLI; GENE CASSETTE; GENE INSERTION; GENETIC MARKER; GENETIC SELECTION; MOLECULAR BIOLOGY; NONHUMAN; PENICILLIUM CHRYSOGENUM; PLASMID; POLYMERASE CHAIN REACTION; PROTEIN EXPRESSION; PROTEIN MODIFICATION; PROTEIN PROCESSING; PROTEIN STRUCTURE; PROTEIN TARGETING; SACCHAROMYCES CEREVISIAE; SCHIZOSACCHAROMYCES POMBE; SEQUENCE TAGGED SITE; YEAST; BIOLOGICAL MODEL; CHEMISTRY; GENETIC RECOMBINATION; GENETICS; METABOLISM; METHODOLOGY; MOLECULAR CLONING; POLYACRYLAMIDE GEL ELECTROPHORESIS; WESTERN BLOTTING;

PENICILLIUM CHRYSOGENUM; SCHIZOSACCHAROMYCES POMBE;

BLOTTING, WESTERN; CALMODULIN; CLONING, MOLECULAR; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; GENETIC MARKERS; GREEN FLUORESCENT PROTEINS; HISTIDINE; HYDRO-LYASES; INTEINS; KANAMYCIN KINASE; MODELS, GENETIC; OLIGOPEPTIDES; PENICILLIUM CHRYSOGENUM; PHOSPHOTRANSFERASES (ALCOHOL GROUP ACCEPTOR); RECOMBINANT FUSION PROTEINS; RECOMBINATION, GENETIC; SACCHAROMYCES CEREVISIAE PROTEINS; SCHIZOSACCHAROMYCES POMBE PROTEINS;

EID: 79959544267     PISSN: None     EISSN: 14726750     Source Type: Journal    
DOI: 10.1186/1472-6750-11-71     Document Type: Article

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