Study of the PDK1/AKT signaling pathway using selective PDK1 inhibitors, HCS, and enhanced biochemical assays

Analytical Biochemistry

Volumn 414, Issue 2, 2011, Pages 179-186

  Hofler, Alexandra ^a   Nichols, Tim ^a   Grant, Stephan ^a   Lingardo, Laura ^a   Esposito, Edward A ^b   Gridley, Scott ^b   Murphy, Sean T ^a   Kath, John C ^a   Cronin, Ciarán N ^a   Kraus, Michelle ^a   Alton, Gordon ^a   Xie, Zhi ^a   Sutton, Scott ^a   Gehring, Mike ^a   Ermolieff, Jacques ^a  

^a PFIZER INC   (United States)

^b Blue Sky Biotech Inc   (United States)

Author keywords

AKT; Kinase inhibitor; PDK1; Signal transduction; Template directed assembly (TDA)

Indexed keywords

BACTERIOPHAGES; CARBONYL COMPOUNDS; CELL MEMBRANES; CELL PROLIFERATION; CHEMICAL ACTIVATION; ENZYMES; SIGNAL TRANSDUCTION;

BIOCHEMICAL METHODS; CANCER CELL GROWTH; KINASE INHIBITORS; PDK1; PHOSPHATIDYLINOSITOL; PLECKSTRIN HOMOLOGY DOMAINS; SIGNALING PATHWAYS; TEMPLATE-DIRECTED ASSEMBLY;

CELL SIGNALING;

CARBONYL 4 AMINOPYRROLOPYRIMIDINE; CELL PROTEIN; ENZYME INHIBITOR; GREEN FLUORESCENT PROTEIN; NICKEL COMPLEX; PF 4350677; PF 5168899; PF 5168961; PF 518899; PF 5201534; PHOSPHATIDYLINOSITOL 3,4,5 TRISPHOSPHATE; PHOSPHOINOSITIDE DEPENDENT PROTEIN KINASE 1; PLECKSTRIN; PROTEIN FOX03A; PROTEIN KINASE B; PROTEIN KINASE B BETA; PYRROLOPYRIMIDINE DERIVATIVE; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CELL ASSAY; CELL NUCLEUS; CYTOPLASM; ENZYME ACTIVATION; ENZYME ACTIVITY; ENZYME INHIBITION; LIPID VESICLE; MOLECULAR INTERACTION; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN PHOSPHORYLATION; SIGNAL TRANSDUCTION;

EID: 79956100601     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2011.03.013     Document Type: Article

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