Fluorescence-based fixative and vital staining of lipid droplets in Caenorhabditis elegans reveal fat stores using microscopy and flow cytometry approaches

Journal of Lipid Research

Volumn 52, Issue 6, 2011, Pages 1281-1293

  Klapper, Maja ^a   Ehmke, Madeleine ^a   Palgunow, Daniela ^a   Böhme, Mike ^a   Matthäus, Christian ^b   Bergner, Gero ^b   Dietzek, Benjamin ^b   Popp, Jürgen ^b   Döring, Frank ^a  

^a UNIVERSITY OF KIEL   (Germany)

^b LEIBNIZ INSTITUTE OF PHOTONIC TECHNOLOGY   (Germany)

Author keywords

C. Elegans; CARS microscopy; Fatty acid; Fatty acid metabolism; Fluorescence microscopy; Obesity; Triglycerides

Indexed keywords

BODIPY 493; BODIPY 503; DYE; FAT DROPLET; TRANSFORMING GROWTH FACTOR BETA; UNCLASSIFIED DRUG;

ACCURACY; ARTICLE; BODY COMPOSITION; BODY FAT DISTRIBUTION; CAENORHABDITIS ELEGANS; CELL ORGANELLE; COHERENT ANTI STOKES RAMAN SCATTERING MICROSCOPY; CONTROLLED STUDY; DIET RESTRICTION; FEASIBILITY STUDY; FLOW CYTOMETRY; FLUORESCENCE MICROSCOPY; GERM LINE; LIPID ANALYSIS; LIPID STORAGE; LYSOSOME; MICROSCOPY; MUTANT; NONHUMAN; OBESITY; PRIORITY JOURNAL; RAMAN MICROSPECTROSCOPY; SENSITIVITY AND SPECIFICITY; SPECTROSCOPY; VITAL STAIN;

ADIPOSE TISSUE; ADIPOSITY; ANIMALS; AZO COMPOUNDS; BORON COMPOUNDS; CAENORHABDITIS ELEGANS; DISEASE MODELS, ANIMAL; FIXATIVES; FLOW CYTOMETRY; FLUORESCENCE; GERM-LINE MUTATION; HIGH-THROUGHPUT SCREENING ASSAYS; LIPID METABOLISM; MICROSCOPY; OBESITY; SPECIES SPECIFICITY; SPECTRUM ANALYSIS, RAMAN; STAINING AND LABELING; TRANSFORMING GROWTH FACTOR BETA;

EID: 79956046932     PISSN: 00222275     EISSN: 15397262     Source Type: Journal    
DOI: 10.1194/jlr.D011940     Document Type: Article

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