A peptide-based target screen implicates the protein kinase CK2 in the global regulation of caspase signaling

Science Signaling

Volumn 4, Issue 172, 2011, Pages

  Duncan, James S ^a   Turowec, Jacob P ^a   Duncan, Kelly E ^a   Vilk, Greg ^a   Wu, Chenggang ^a   Lüscher, Bernhard ^b   Li, Shawn S C ^a   Gloor, Greg B ^a   Litchfield, David W ^a  

^a UNIVERSITY OF WESTERN ONTARIO   (Canada)

^b RWTH AACHEN UNIVERSITY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

CASEIN KINASE II; CASPASE 3; CASPASE 8; CASPASE 9; PROTEOME; CASPASE;

APOPTOSIS; ARTICLE; ENZYME ANALYSIS; ENZYME INHIBITION; ENZYME PHOSPHORYLATION; HUMAN; MASS SPECTROMETRY; MOLECULAR RECOGNITION; PRIORITY JOURNAL; PROTEIN CLEAVAGE; PROTEIN DEGRADATION; REGULATORY MECHANISM; SIGNAL TRANSDUCTION; AMINO ACID SEQUENCE; ENZYME SPECIFICITY; HYDROLYSIS; METABOLISM; PHOSPHORYLATION; VALIDATION STUDY;

AMINO ACID SEQUENCE; APOPTOSIS; CASEIN KINASE II; CASPASES; HYDROLYSIS; MASS SPECTROMETRY; PHOSPHORYLATION; SIGNAL TRANSDUCTION; SUBSTRATE SPECIFICITY;

EID: 79955900608     PISSN: 19450877     EISSN: 19379145     Source Type: Journal    
DOI: 10.1126/scisignal.2001682     Document Type: Article

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63. Acknowledgments: We thank X. Jiang for the synthesis of peptide arrays and soluble peptides used in the CSI assays, and K. Jurcic and P. Pittock in the facilities of the London Regional Proteomics Centre for sample processing and mass spectrometry. We also thank K. Garside, N. St. Denis, and L. Gyenis for helpful discussions. Funding: This work was funded by grants from the Canadian Institutes of Health Research (to D.W.L.) and Genome Canada (to S.S.-C.L. and D.W.L.). J.S.D. was jointly supported by a Canadian Institute of Health Research-Canadian Graduate Scholarship and a Canadian Institute of Health Research-UWO Strategic Training Initiative in Cancer Research and Technology Transfer scholarship. J.P.T. was supported by scholarships from the Ontario Graduate Scholarship and Ontario Graduate Scholarship Science and Technology programs. Author contributions: J.S.D. and J.P.T. performed most of the experimental studies and data analysis, with additional contributions from K.E.D. and G.V. Peptides and peptide arrays were synthesized by C.W., and computational studies and analyses were performed by G.B.G. Study design was led by J.S.D., J.P.T., G.V., and D.W.L., with additional support from B.L., G.B.G., and S.S.-C.L. Supervisory support was provided by D.W.L., with contributions from B.L., G.B.G., and S.S.-C.L. All authors contributed to the final preparation and editing of the manuscript. Competing interests: The authors declare that they have no competing interests.