A combination of (ω-3) polyunsaturated fatty acids, polyphenols and L-carnitine reduces the plasma lipid levels and increases the expression of genes involved in fatty acid oxidation in human peripheral blood mononuclear cells and HepG2 cells

Annals of Nutrition and Metabolism

Volumn 58, Issue 2, 2011, Pages 133-140

  Radler, Ulla ^a   Stangl, Herbert ^a   Lechner, Sigrid ^a   Lienbacher, Gerhard ^c   Krepp, Rainer ^c   Zeller, Eduard ^c   Brachinger, Martin ^c   Eller Berndl, Doris ^c   Fischer, Andreas ^c   Anzur, Christian ^d   Schoerg, Gerhard ^d   Mascher, Daniel ^e   Laschan, Claudia ^b   Anderwald, Christian ^a   Lohninger, Alfred ^a  

^a MEDICAL UNIVERSITY OF VIENNA   (Austria)

^b HANUSCH HOSPITAL   (Austria)

^c Health Care Center Pielachtal   (Austria)

^d UNIVERSITY OF APPLIED SCIENCES WIENER NEUSTADT   (Austria)

^e Pharm Analyt Laboratory GmbH   (Austria)

Author keywords

3 polyunsaturated fatty acids; Carnitine; Gene expression; HepG2 cells; Peripheral blood mononuclear cells; Plasma lipids; Polyphenols

Indexed keywords

CARNITINE; CARNITINE ACETYLTRANSFERASE; CARNITINE PALMITOYLTRANSFERASE I; CARNITINE PALMITOYLTRANSFERASE IA; CARNITINE PALMITOYLTRANSFERASE IB; FATTY ACID; MESSENGER RNA; OMEGA 3 FATTY ACID; ORGANIC CATION TRANSPORTER 2; PEROXISOME PROLIFERATOR ACTIVATED RECEPTOR ALPHA; PLACEBO; POLYPHENOL DERIVATIVE; TRIACYLGLYCEROL; UNCLASSIFIED DRUG; YOGHURT;

ADULT; ARTICLE; CELL STRAIN HEPG2; CLINICAL ARTICLE; CONTROLLED STUDY; DIET SUPPLEMENTATION; DOUBLE BLIND PROCEDURE; FATTY ACID BLOOD LEVEL; FATTY ACID OXIDATION; FEMALE; GENE EXPRESSION; HUMAN; HUMAN CELL; HYPERLIPIDEMIA; LOW FAT DIET; MALE; OBESITY; PERIPHERAL BLOOD MONONUCLEAR CELL; PRIORITY JOURNAL; RANDOMIZED CONTROLLED TRIAL; TRIACYLGLYCEROL BLOOD LEVEL;

ADULT; CARNITINE; DOUBLE-BLIND METHOD; FATTY ACIDS; FATTY ACIDS, OMEGA-3; FEMALE; FLAVONOIDS; FUNCTIONAL FOOD; HEP G2 CELLS; HEPATOCYTES; HUMANS; HYPERLIPIDEMIAS; HYPOLIPIDEMIC AGENTS; LEUKOCYTES, MONONUCLEAR; LIPIDS; MALE; MIDDLE AGED; OVERWEIGHT; OXIDATION-REDUCTION; PHENOLS; PPAR ALPHA; RNA, MESSENGER; UP-REGULATION;

EID: 79955441980     PISSN: 02506807     EISSN: 14219697     Source Type: Journal    
DOI: 10.1159/000327150     Document Type: Article

References (42)

1. Boden G, Shulman GI: Free fatty acids in obesity and type 2 diabetes: Defining their role in the development of insulin resistance and beta-cell dysfunction. Eur J Clin Invest 2002; 32(suppl 3):14-23. (Pubitemid 41704996)
2. Anderwald C, Roden M: Adipotoxicity and the insulin resistance syndrome. Pediatr Endocrinol Rev 2004; 1: 310-319. (Pubitemid 40204079)
3. Browning JD, Horton JD: Molecular mediators of hepatic steatosis and liver injury. J Clin Invest 2004; 114: 147-152. (Pubitemid 39071617)
4. Coppack SW, Jensen MD, Miles JM: In vivo regulation of lipolysis in humans. J Lipid Res 1994; 35: 177-193. (Pubitemid 24050431)
5. Felber JP, Magnenat G, Casthelaz M, et al: Carbohydrate and lipid oxidation in normal and diabetic subjects. Diabetes 1977; 26: 693-699. (Pubitemid 8149657)
6. Noland RC, Koves TR, Seiler SE, et al: Carnitine insufficiency caused by aging and overnutrition compromises mitochondrial performance and metabolic control. J Biol Chem 2009; 284: 22840-22852.
7. Seccombe DW, James L, Hahn P, Jones E: L -carnitine treatment in the hyperlipidemic rabbit. Metabolism 1987; 36: 1192-1196.
8. Adams SH, Hoppel CL, Lok KH, et al: Plasma acylcarnitine profiles suggest incomplete long-chain fatty acid beta-oxidation and altered tricarboxylic acid cycle activity in type 2 diabetic African-American women. J Nutr 2009; 139: 1073-1081.
9. Godarova A, Litzlbauer E, Brunner S, Agu AC, Lohninger A, Hofbauer R: L -Carnitine regulates mRNA expression levels of the carnitine acyltransferases -CPT1A, CPT2 and CRAT. Chem Monthly 2005; 136: 1349-1363. (Pubitemid 41095365)
10. Karlic H, Lohninger S, Koeck T, Lohninger A: Dietary l-carnitine stimulates carnitine acyltransferases in the liver of aged rats. J Histochem Cytochem 2002; 50: 205-212. (Pubitemid 34106759)
11. Zeibig J, Karlic H, Lohninger A, Damsgaard R, Smekal G: Do blood cells mimic gene expression profile alterations known to occur in muscular adaptation to endurance training? Eur J Appl Physiol 2005; 95: 96-104. (Pubitemid 43008902)
12. Ramsay RR, Zammit VA: Carnitine acyltransferases and their influence on CoA pools in health and disease. Mol Aspects Med 2004; 25: 475-493. (Pubitemid 39195072)
13. Mandard S, Muller M, Kersten S: Peroxisome proliferator-activated receptor alpha target genes. Cell Mol Life Sci 2004; 61: 393-416. (Pubitemid 38316578)
14. Bunger M, Hooiveld GJ, Kersten S, Muller M: Exploration of PPAR functions by microarray technology -a paradigm for nutrigenomics. Biochim Biophys Acta 2007; 1771: 1046-1064. (Pubitemid 47198550)
15. Mori T, Kondo H, Hase T, Tokimitsu I, Murase T: Dietary fish oil upregulates intestinal lipid metabolism and reduces body weight gain in C57BL/6J mice. J Nutr 2007; 137: 2629-2634. (Pubitemid 350255289)
16. Siddiqui RA, Harvey KA, Zaloga GP: Modulation of enzymatic activities by n-3 polyunsaturated fatty acids to support cardiovascular health. J Nutr Biochem 2008; 19: 417-437. (Pubitemid 351783409)
17. Bouwens M, Afman LA, Muller M: Fasting induces changes in peripheral blood mononuclear cell gene expression profiles related to increases in fatty acid beta-oxidation: Functional role of peroxisome proliferator activated receptor alpha in human peripheral blood mononuclear cells. Am J Clin Nutr 2007; 86: 1515-1523. (Pubitemid 350126259)
18. Bouwens M, Afman LA, Muller M: Activation of peroxisome proliferator-activated receptor alpha in human peripheral blood mononuclear cells reveals an individual gene expression profile response. BMC Genom 2008; 9: 262.
19. Brown HR, Ni H, Benavides G, et al: Correlation of simultaneous differential gene expression in the blood and heart with known mechanisms of adriamycin-induced cardiomyopathy in the rat. Toxicol Pathol 2002; 30: 452-469.
20. Liu J, Atamna H, Kuratsune H, Ames BN: Delaying brain mitochondrial decay and aging with mitochondrial antioxidants and metabolites. Ann NY Acad Sci 2002; 959: 133-166. (Pubitemid 34457559)
21. Winer J, Jung CK, Shackel I, Williams PM: Development and validation of real-time quantitative reverse transcriptase-polymerase chain reaction for monitoring gene expression in cardiac myocytes in vitro. Analyt Biochem 1999; 270: 41-49. (Pubitemid 29230718)
22. Suzuki T, Higgins PJ, Crawford DR: Control selection for RNA quantitation. Biotechniques 2000; 29: 332-337. (Pubitemid 30658285)
23. Zhang X, Ding L, Sandford AJ: Selection of reference genes for gene expression studies in human neutrophils by real-time PCR. BMC Mol Biol 2005; 6: 4.
24. Lohninger A, Preis P, Linhart L, Sommoggy SV, Landau M, Kaiser E: Determination of plasma free fatty acids, free cholesterol, cholesteryl esters, and triacylglycerols directly from total lipid extract by capillary gas chromatography. Anal Biochem 1990; 186: 243-250. (Pubitemid 20153499)
25. Zammit VA, Waterman IJ, Topping D, Mc-Kay G: Insulin stimulation of hepatic triacylglycerol secretion and the etiology of insulin resistance. J Nutr 2001; 131: 2074-2077. (Pubitemid 32720632)
26. Bonnefont JP, Djouadi F, Prip-Buus C, Gobin S, Munnich A, Bastin J: Carnitine palmitoyltransferases 1 and 2: Biochemical, molecular and medical aspects. Mol Aspects Med 2004; 25: 495-520. (Pubitemid 39195073)
27. Ramsay RR, Gandour RD, van der Leij FR: Molecular enzymology of carnitine transfer and transport. Biochim Biophys Acta 2001; 1546: 21-43. (Pubitemid 32217800)
28. Ringseis R, Luci S, Spielmann J, et al: Clofibrate treatment up-regulates novel organic cation transporter (OCTN)-2 in tissues of pigs as a model of non-proliferating species. Eur J Pharmacol 2008; 583: 11-17.
29. Mingrone G: Carnitine in type 2 diabetes. Ann NY Acad Sci 2004; 1033: 99-107. (Pubitemid 39656742)
30. Horiuchi M, Kobayashi K, Tomomura M, et al: Carnitine administration to juvenile visceral steatosis mice corrects the suppressed expression of urea cycle enzymes by normalizing their transcription. J Biol Chem 1992; 267: 5032-5035.
31. Makowski L, Noland RC, Koves TR, et al: Metabolic profiling of PPARalpha-/-mice reveals defects in carnitine and amino acid homeostasis that are partially reversed by oral carnitine supplementation. FASEB J 2009; 23: 586-604.
32. Hsu SC, Huang CJ: Reduced fat mass in rats fed a high oleic acid-rich safflower oil diet is associated with changes in expression of hepatic PPARalpha and adipose SREBP-1c-regulated genes. J Nutr 2006; 136: 1779-1785.
33. Arduini A, Bonomini M, Savica V, Amato A, Zammit V: Carnitine in metabolic disease: Potential for pharmacological intervention. Pharmacol Ther 2008; 120: 149-156.
34. Koves TR, Ussher JR, Noland RC, et al: Mitochondrial overload and incomplete fatty acid oxidation contribute to skeletal muscle insulin resistance. Cell Metab 2008; 7: 45-56.
35. Moder M, Kiessling A, Loster H, Bruggemann L: The pattern of urinary acylcarnitines determined by electrospray mass spectrometry: A new tool in the diagnosis of diabetes mellitus. Anal Bioanal Chem 2003; 375: 200-210. (Pubitemid 41201295)
36. Stevenson DE, Hurst RD: Polyphenolic phytochemicals -just antioxidants or much more? Cell Mol Life Sci 2007; 64: 2900-2916. (Pubitemid 351152245)
37. Chew BP, Park JS: Carotenoid action on the immune response. J Nutr 2004; 134: 257S-261S. (Pubitemid 38057459)
38. Halvorsen BL, Holte K, Myhrstad MC, et al: A systematic screening of total antioxidants in dietary plants. J Nutr 2002; 132: 461-471. (Pubitemid 34208750)
39. Shin ES, Cho SY, Lee EH, Lee SJ, Chang IS, Lee TR: Positive regulation of hepatic carnitine palmitoyl transferase 1A (CPT1A) activities by soy isoflavones and L -carnitine. Eur J Nutr 2006; 45: 159-164.
40. Jacobson TA: Role of n-3 fatty acids in the treatment of hypertriglyceridemia and cardiovascular disease. Am J Clin Nutr 2008; 87: 1981S-1990S. (Pubitemid 351872679)
41. Hanwell HE, Kay CD, Lampe JW, Holub BJ, Duncan AM: Acute fish oil and soy isoflavone supplementation increase postprandial serum (n-3) polyunsaturated fatty acids and isoflavones but do not affect triacylglycerols or biomarkers of oxidative stress in overweight and obese hypertriglyceridemic men. J Nutr 2009; 139: 1128-1134.
42. Jenkins DJ, Kendall CW, Marchie A, et al: Direct comparison of a dietary portfolio of cholesterol-lowering foods with a statin in hypercholesterolemic participants. Am J Clin Nutr 2005; 81: 380-387. (Pubitemid 43073339)