Clofibrate treatment up-regulates novel organic cation transporter (OCTN)-2 in tissues of pigs as a model of non-proliferating species

European Journal of Pharmacology

Volumn 583, Issue 1, 2008, Pages 11-17

  Ringseis, Robert ^a   Luci, Sebastian ^a   Spielmann, Julia ^a   Kluge, Holger ^a   Fischer, Maren ^a   Geissler, Stefanie ^a   Wen, Gaiping ^a   Hirche, Frank ^a   Eder, Klaus ^a  

^a MARTIN LUTHER UNIVERSITY HALLE WITTENBERG   (Germany)

Author keywords

(Pig); Carnitine; Clofibrate; Novel organic cation transporter; Peroxisome proliferator activated receptor

Indexed keywords

CARNITINE; CLOFIBRATE; GAMMA BUTYROBETAINE; GAMMA BUTYROBETAINE DIOXYGENASE; MESSENGER RNA; ORGANIC CATION TRANSPORTER 2; PEROXISOME PROLIFERATOR ACTIVATED RECEPTOR ALPHA; PEROXISOME PROLIFERATOR ACTIVATED RECEPTOR ALPHA AGONIST;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; GENE EXPRESSION; INTESTINE CELL; LIVER; MALE; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN INDUCTION; PROTEIN LOCALIZATION; RODENT; SKELETAL MUSCLE; SMALL INTESTINE MUCOSA; SWINE; TISSUE DISTRIBUTION; UPREGULATION;

ANIMALS; ANTILIPEMIC AGENTS; BETAINE; BODY WEIGHT; CARNITINE; CLOFIBRATE; EATING; ENTEROCYTES; GAMMA-BUTYROBETAINE DIOXYGENASE; GENE EXPRESSION; HOMEOSTASIS; LIVER; MALE; ORGANIC CATION TRANSPORT PROTEINS; PPAR ALPHA; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA; RNA, MESSENGER; SWINE; TISSUE DISTRIBUTION; UP-REGULATION;

EID: 39649106879     PISSN: 00142999     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ejphar.2008.01.008     Document Type: Article

References (50)

1. Braissant O., Foufelle F., Scotto C., Dauca M., and Wahli W. Differential expression of peroxisome proliferator-activated receptors (PPARs): tissue distribution of PPAR-α, -β, and -δ in the adult rat. Endocrinology 137 (1996) 354-366
2. Brandt J.M., Djouadi F., and Kelly D. Fatty acids activate transcription of the muscle carnitine palmitoyltransferase I gene in cardiac myocytes via the peroxisome proliferator-activated receptor alpha. J. Biol. Chem. 273 (1998) 23786-23792
3. Brass E.P. Pivalate-generating prodrugs and carnitine homeostasis in man. Pharmacol. Rev. 54 (2002) 589-598
4. Brass E.P., and Hoppel C.L. Carnitine metabolism in the fasting rat. J. Biol. Chem. 253 (1978) 2688-2693
5. Cheon Y., Nara T.Y., Band M.R., Beever J.E., Wallig M.A., and Nakamura M.T. Induction of overlapping genes by fasting and a peroxisome proliferator in pigs: evidence of functional PPAR alpha in nonproliferating species. Am. J. Physiol., Regul. Integr. Comp. Physiol. 288 (2005) R1525-R1535
6. Cook W.S., Yeldandi A.V., Rao M.S., Hashimoto T., and Reddy J.K. Less extrahepatic induction of fatty acid β-oxidation enzymes by PPARα. Biochem. Biophys. Res. Commun. 278 (2000) 250-257
7. Fan M.Z., Matthews J.C., Etienne N.M., Stoll B., Lackeyram D., and Burrin D.G. Expression of apical membrane l-glutamate transporters in neonatal porcine epithelial cells along the small intestinal crypt-villus axis. Am. J. Physiol.: Gastrointest. Liver Physiol. 287 (2004) G385-G398
8. Frederiksen K.S., Wulff E.M., Sauerberg P., Mogensen J.P., Jeppesen L., and Fleckner J. Prediction of PPAR-alpha ligand-mediated physiological changes using gene expression profiles. J. Lipid Res. 45 (2004) 592-601
9. Grube M., Meyer zu Schwabedissen H.E., Präger D., Haney J., Möritz K.U., Meissner K., Rosskopf D., Eckel L., Böhm M., Jedlitschky G., and Kroemer H.K. Uptake of cardiovascular drugs into the human heart: expression, regulation, and function of the carnitine transporter OCTN2 (SLC22A5). Circulation 113 (2006) 1114-1122
10. He W.S., Nara T.Y., and Nakamura M.T. Delayed induction of delta-6 and delta-5 desaturases by a peroxisome proliferators. Biochem. Biophys. Res. Commun. 299 (2002) 832-838
11. Hirano T., Yasuda S., Osaka Y., Kobayashi M., Itagaki S., and Iseki K. Mechanism of the inhibitory effect of zwitterionic drugs (levofloxacin and grepafloxacin) on carnitine transporter (OCTN2) in Caco-2 cells. Biochim. Biophys. Acta 1758 (2006) 1743-1750
12. Holden P.R., and Tugwood J.D. Peroxisome proliferator-activated receptor alpha: role in rodent liver cancer and species differences. J. Mol. Endocrinol. 22 (1999) 1-8
13. Kato Y., Sugiura M., Sugiura T., Wakayama T., Kubo Y., Kobayashi D., Sai Y., Tamai I., Iseki S., and Tsuji A. Organic cation/carnitine transporter OCTN2 (Slc22a5) is responsible for carnitine transport across apical membranes of small intestinal epithelial cells in mouse. Mol. Pharmacol. 70 (2006) 829-837
14. Kliewer S.A., Forman B.M., Blumberg B., Ong E.S., Borgmeyer U., Mangelsdorf D.J., Umesono K., and Evans R.M. Differential expression and activation of a family of murine peroxisome proliferator-activated receptors. Proc. Natl. Acad. Sci. U. S. A. 91 (1994) 7355-7359
15. Knight B.L., Hebbach A., Hauton D., Brown A.M., Wiggins D., and Patel D.D. A role for PPARα in the control of SREBP activity and lipid synthesis in the liver. Biochem. J. 389 (2005) 413-421
16. Koepsell H., and Endou H. The SLC22 drug transporter family. Pflugers Arch. 447 (2004) 666-676
17. König B., Koch A., Spielmann J., Hilgenfeld C., Stangl G.I., and Eder K. Activation of PPARalpha lowers synthesis and concentration of cholesterol by reduction of nuclear SREBP-2. Biochem. Pharmacol. 73 (2007) 574-585
18. Krey G., Braissant O., L'Horset F., Kalkhoven E., Perroud M., Parker M.G., and Wahli W. Fatty acids, eicosanoids, and hypolipidemic agents identified as ligands of peroxisome proliferator-activated receptors by coactivator-dependent receptor ligand assay. Mol. Endocrinol. 11 (1997) 779-791
19. Lahjouji K., Elimrani I., Lafond J., Leduc L., Qureshi I.A., and Mitchell G.A. l-Carnitine transport in human placental brush-border membranes is mediated by the sodium-dependent organic cation transporter OCTN2. Am. J. Physiol. Cell Physiol. 287 (2004) C263-C269
20. Lahjouji K., Mitchell G.A., and Qureshi I.A. Carnitine transport by organic cation transporters and systemic carnitine deficiency. Mol. Genet. Metab. 73 (2001) 287-297
21. Luci S., Geissler S., König B., Koch A., Stangl G.I., Hirche F., and Eder K. PPARα agonists up-regulate organic cation transporters in rat liver cells. Biochem. Biophys. Res. Commun. 24 (2006) 704-708
22. Luci S., Giemsa B., Kluge H., and Eder K. Clofibrate causes an upregulation of PPARα target genes but does not alter expression of SREBP target genes in liver and adipose tissue of pigs. Am. J. Physiol., Regul. Integr. Comp. Physiol. 293 (2007) R70-R77
23. Mascaro C., Acosta E., Ortiz J.A., Marrero P.F., Hegardt F.G., and Haro D. Control of human muscle-type carnitine palmitoyltransferase I gene transcription by peroxisome proliferator-activated receptor. J. Biol. Chem. 273 (1998) 8560-8563
24. McGarry J.D., and Brown N.F. The mitochondrial carnitine palmitoyltransferase system. From concept to molecular analysis. Eur. J. Biochem. 244 (1997) 1-14
25. McGarry J.D., Robles-Valdes C., and Foster D.W. Role of carnitine in hepatic ketogenesis. Proc. Natl. Acad. Sci. U. S. A. 72 (1975) 4385-4388
26. National Research Council. Nutrient Requirement of Swine. Tenth Revised Edition (1998), National Academie of Sciences, Washington DC
27. Ohashi R., Tamai I., Nezu Ji J., Nikaido H., Hashimoto N., Oku A., Sai Y., Shimane M., and Tsuji A. Molecular and physiological evidence for multifunctionality of carnitine/organic cation transporter OCTN2. Mol. Pharmacol. 59 (2001) 358-366
28. +-dependent carnitine transport by organic cation transporter (OCTN2): its pharmacological and toxicological relevance. J. Pharmacol. Exp. Ther. 291 (1999) 778-784
29. Paul H.S., and Adibi S.A. Paradoxical effects of clofibrate on liver and muscle metabolism in rats. Induction of myotonia and alteration of fatty acid and glucose oxidation. J. Clin. Invest. 64 (1979) 405-412
30. Peffer P.L., Lin X., and Odle J. Hepatic beta-oxidation and carnitine palmitoyltransferase I in neonatal pigs after dietary treatments of clofibric acid, isoproterenol, and medium-chain triglycerides. Am. J. Physiol., Regul. Integr. Comp. Physiol. 288 (2005) R1518-R1524
31. Peters J.M., Cheung C., and Gonzalez F.J. Peroxisome proliferator-activated receptor-alpha and liver cancer: where do we stand?. J. Mol. Med. 83 (2005) 774-785
32. Pfaffl M.W. A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res. 29 (2001) e45
33. Rebouche C.J., and Seim H. Carnitine metabolism and its regulation in microorganisms and mammals. Annu. Rev. Nutr. 18 (1998) 39-61
34. Ringseis R., Pösel S., Hirche F., and Eder K. Treatment with pharmacological peroxisome proliferator-activated receptor α agonist clofibrate causes upregulation of organic cation transporter 2 in liver and small intestine of rats. Pharmacol. Res. 56 (2007) 175-183
35. Ruan J., Guo Y., Li H., Hu Y., Song F., Huang X., Kristiensen K., Bolund L., and Wang J. PigGIS: Pig Genomic Informatics System. Nucleic Acids Res. 35 Database issue (2007) D654-D657
36. Schoonjans K., Peinado-Onsurbe J., Lefebvre A.M., Heyman R.A., Briggs M., Deeb S., Staels B., and Auwerx J. PPARα and PPARγ activators direct a distinct tissue-specific transcriptional response via a PPRE in the lipoprotein lipase gene. Embo J. 15 (1996) 5336-5348
37. Sekine T., Kusuhara H., Utsunomiya-Tate N., Tsuda M., Sugiyama Y., Kanai Y., and Endou H. Molecular cloning and characterization of high-affinity carnitine transporter from rat intestine. Biochem. Biophys. Res. Commun. 251 (1998) 586-591
38. Slitt A.L., Cherrington N.J., Hartley D.P., Leazer M.T., and Klaassen C.D. Tissue distribution and renal developmental changes in rat organic cation transporter mRNA levels. Drug Metab. Dispos. 30 (2002) 212-219
39. Steiber A., Kerner J., and Hoppel C.L. Carnitine: a nutritional, biosynthetic, and functional perspective. Mol. Asp. Med. 25 (2004) 455-473
40. Sundvold H., Grindflek E., and Lien S. Tissue distribution of porcine peroxisome proliferator-activated receptor α: detection of an alternatively spliced mRNA. Gene 273 (2001) 105-113
41. Tamai I., Ohashi R., Nezu J., Yabuuchi H., Oku A., Shimane M., Sai Y., and Tsuji A. Molecular and functional identification of sodium ion-dependent, high affinity human carnitine transporter OCTN2. J. Biol. Chem. 273 (1998) 20378-20382
42. Tamai I., Ohashi R., Nezu J.I., Sai Y., Kobayashi D., Oku A., Shimane M., and Tsuji A. Molecular and functional characterization of organic cation/carnitine transporter family in mice. J. Biol. Chem. 275 (2000) 40064-40072
43. Tamai I., Yabuuchi H., Nezu J., Sai Y., Oku A., Shimane M., and Tsuji A. Cloning and characterization of a novel human pH-dependent organic cation transporter, OCTN1. FEBS Lett. 419 (1997) 107-111
44. Tein I. Carnitine transport: pathophysiology and metabolism of known defects. J. Inherit. Metab. Dis. 26 (2003) 147-169
45. Van Vlies N., Wanders R.J., and Vaz F.M. Measurement of carnitine biosynthesis enzyme activities by tandem mass spectrometry: differences between the mouse and the rat. Anal. Biochem. 354 (2006) 132-139
46. Van Vlies N., Ferdinandusse S., Turkenburg M., Wanders R.J., and Vaz F.M. PPARα-activation results in enhanced carnitine biosynthesis and OCTN2-mediated hepatic carnitine accumulation. Biochim. Biophys. Acta 1767 9 (2007) 1134-1142
47. Vaz F.M., and Wanders R.J. Carnitine biosynthesis in mammals. Biochem. J. 361 (2002) 417-429
48. Wu X., Huang W., Prasad P.D., Seth P., Rajan D.P., Leibach F.H., Chen J., Conway S.J., and Ganapathy V. Functional characteristics and tissue distribution pattern of organic cation transporter 2 (OCTN2), an organic cation/carnitine transporter. J. Pharmacol. Exp. Ther. 290 (1999) 1482-1492
49. Yu X.X., Odle J., and Drackley J.K. Differential induction of peroxisomal beta-oxidation enzymes by clofibric acid and aspirin in piglet tissues. Am. J. Physiol., Regul. Integr. Comp. Physiol. 281 (2001) R1553-R1561
50. Zoete V., Grosdidier A., and Michielin O. Peroxisome proliferator-activated receptor structures: ligand specificity, molecular switch and interactions with regulators. Biochim. Biophys. Acta 1771 (2007) 915-925