Thioredoxin mediates oxidation-dependent phosphorylation of CRMP2 and growth cone collapse

Science Signaling

Volumn 4, Issue 170, 2011, Pages

  Morinaka, Akifumi ^a   Yamada, Mayumi ^b,c   Itofusa, Rurika ^d   Funato, Yosuke ^a   Yoshimura, Yuta ^a   Nakamura, Fumio ^e   Yoshimura, Takeshi ^f,g   Kaibuchi, Kozo ^f   Goshima, Yoshio ^e   Hoshino, Mikio ^b   Kamiguchi, Hiroyuki ^d   Miki, Hiroaki ^a  

^a OSAKA UNIVERSITY   (Japan)

^b NATIONAL INSTITUTE OF NEUROSCIENCE   (Japan)

^c KYOTO UNIVERSITY GRADUATE SCHOOL OF MEDICINE   (Japan)

^d RIKEN BRAIN SCIENCE INSTITUTE   (Japan)

^e YOKOHAMA CITY UNIVERSITY   (Japan)

^f NAGOYA UNIVERSITY GRADUATE SCHOOL OF MEDICINE   (Japan)

^g NATIONAL INSTITUTE FOR PHYSIOLOGICAL SCIENCES   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

COLLAPSIN RESPONSE MEDIATOR PROTEIN 2; DISULFIDE; GLYCOGEN SYNTHASE KINASE 3; HOMODIMER; HYDROGEN PEROXIDE; SEMAPHORIN 3A; THIOREDOXIN; COLLAPSIN RESPONSE MEDIATOR PROTEIN-2; MICAL1 PROTEIN, MOUSE; MICROTUBULE ASSOCIATED PROTEIN; MIXED FUNCTION OXIDASE; NERVE PROTEIN; RECOMBINANT PROTEIN; SEMA3A PROTEIN, MOUSE; SIGNAL PEPTIDE; SMALL INTERFERING RNA;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; COMPLEX FORMATION; CONTROLLED STUDY; DISULFIDE BOND; GROWTH CONE; IN VITRO STUDY; MOUSE; NONHUMAN; OXIDATION; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION; PROTEIN PROTEIN INTERACTION; PROTEIN PURIFICATION; REGULATORY MECHANISM; ANIMAL; CELL STRAIN 3T3; CELL STRAIN COS1; CERCOPITHECUS; CHICK EMBRYO; DRUG ANTAGONISM; FEMALE; GENE SILENCING; GENETICS; HUMAN; METABOLISM; NUCLEOTIDE SEQUENCE; OXIDATION REDUCTION REACTION; PHOSPHORYLATION; PREGNANCY; PRENATAL DEVELOPMENT; SIGNAL TRANSDUCTION; SPINAL GANGLION;

ANIMALS; BASE SEQUENCE; CERCOPITHECUS AETHIOPS; CHICK EMBRYO; COS CELLS; FEMALE; GANGLIA, SPINAL; GENE KNOCKDOWN TECHNIQUES; GROWTH CONES; HUMANS; HYDROGEN PEROXIDE; INTERCELLULAR SIGNALING PEPTIDES AND PROTEINS; MICE; MICROTUBULE-ASSOCIATED PROTEINS; MIXED FUNCTION OXYGENASES; NERVE TISSUE PROTEINS; NIH 3T3 CELLS; OXIDATION-REDUCTION; PHOSPHORYLATION; PREGNANCY; RECOMBINANT PROTEINS; RNA, SMALL INTERFERING; SEMAPHORIN-3A; SIGNAL TRANSDUCTION; THIOREDOXINS;

EID: 79955430653     PISSN: 19450877     EISSN: 19379145     Source Type: Journal    
DOI: 10.1126/scisignal.2001127     Document Type: Article

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55. Acknowledgments: We thank S. Ohmi and H. Fukuda for technical help with mass spectrometry; J. Takagi, N. Yasui, and T. Tojima for helpful discussion; and T. Kawauchi for technical comments. Funding: This study was supported in part by Grant-in-Aid for Scientific Research from the Japan Society for the Promotion of Science (H.M.) and the Ministry of Education, Culture, Sports, Science and Technology of Japan (H.M.). Author contributions: A.M. and H.M. are responsible for the overall study design. A.M. performed the experimental work and data analysis. M.Y. and M.H. performed in utero electroporation. Y.F. and Y.Y. trained A.M. in basic experimental procedures. R.I., H.K., F.N., and Y.G. trained A.M. in experiments with DRG neurons. T.Y. and K.K. generated anti-p-CRMP2 antibody. A.M. and H.M. wrote the paper with the help of the other authors. Competing interests: The authors declare that they do not have any competing financial, personal, or professional interest.