Genetically encoded fluorescent indicator for intracellular hydrogen peroxide

Nature Methods

Volumn 3, Issue 4, 2006, Pages 281-286

  Belousov, Vsevolod V ^a   Fradkov, Arkady F ^a   Lukyanov, Konstantin A ^a   Staroverov, Dmitry B ^a,b   Shakhbazov, Konstantin S ^c   Terskikh, Alexey V ^c,d   Lukyanov, Sergey ^a  

^a SHEMYAKIN OVCHINNIKOV INSTITUTE OF BIOORGANIC CHEMISTRY   (Russian Federation)

^b Evrogen JSC   (Russian Federation)

^c EPFL   (Switzerland)

^d BURNHAM INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

FLUORESCENT DYE; HYDROGEN PEROXIDE; NERVE GROWTH FACTOR; TUMOR NECROSIS FACTOR RELATED APOPTOSIS INDUCING LIGAND; YELLOW FLUORESCENT PROTEIN;

ANIMAL CELL; APOPTOSIS; ARTICLE; CELL LEVEL; CELL SHAPE; CELL STIMULATION; CELL STRAIN; CONTROLLED STUDY; CYTOPLASM; HELA CELL; HUMAN; HUMAN CELL; MEMBRANE POTENTIAL; MITOCHONDRIAL MEMBRANE; MITOCHONDRION; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; RAT; SENSITIVITY ANALYSIS;

ANIMALS; APOPTOSIS REGULATORY PROTEINS; BACTERIAL PROTEINS; BIOSENSING TECHNIQUES; CYTOPLASM; DNA-BINDING PROTEINS; ESCHERICHIA COLI PROTEINS; GENETIC CODE; HELA CELLS; HUMANS; HYDROGEN PEROXIDE; INTRACELLULAR MEMBRANES; LUMINESCENT PROTEINS; MITOCHONDRIA; NERVE GROWTH FACTORS; PC12 CELLS; PROKARYOTIC CELLS; RATS; REPRESSOR PROTEINS; SENSITIVITY AND SPECIFICITY; TIME FACTORS; TRANSCRIPTION FACTORS;

ANIMALIA; PROKARYOTA;

EID: 33645283923     PISSN: 15487091     EISSN: 15491676     Source Type: Journal    
DOI: 10.1038/nmeth866     Document Type: Article

References (23)

1. Droge, W. Free radicals in the physiological control of cell function. Physiol. Rev. 82, 47-95 (2002).
2. Crow, J.P. Dichlorodihydrofluorescein and dihydrorhodamine 123 are sensitive indicators of peroxynitrite in vitro: Implications for intracellular measurement of reactive nitrogen and oxygen species. Nitric Oxide 1, 145-157 (1997).
3. Marchesi, E., Rota, C., Fann, Y.C., Chignell, C.F. & Mason, R.P. Photoreduction of the fluorescent dye 2′-7′-dichlorofluorescein: A spin trapping and direct electron spin resonance study with implications for oxidative stress measurements. Free Radic. Biol. Med. 26, 148-161 (1999).
4. Rota, C., Fann, Y.C. & Mason, R.P. Phenoxyl free radical formation during the oxidation of the fluorescent dye 2′,7′-dichlorofluorescein by horseradish peroxidase. Possible consequences for oxidative stress measurements. J. Biol. Chem. 274, 28161-28168 (1999).
5. Griesbeck, O. Fluorescent proteins as sensors for cellular functions. Curr. Opin. Neurobiol. 14, 636-641 (2004).
6. Zhang, J., Campbell, R.E., Ting, A.Y. & Tsien, R.Y. Creating new fluorescent probes for cell biology. Nat. Rev. Mol. Cell Biol. 3, 906-918 (2002).
7. Nagai, T., Sawano, A., Park, E.S. & Miyawaki, A. Circularly permuted green fluorescent proteins engineered to sense Ca2+. Proc. Natl. Acad. Sci. USA 98, 3197-3202 (2001).
8. Zheng, M., AsLund, F. & Storz, G. Activation of the OxyR transcription factor by reversible disulfide bond formation. Science 279, 1718-1721 (1998).
9. Choi, H. et al. Structural basis of the redox switch in the OxyR transcription factor. Cell 105, 103-13 (2001).
10. Aslund, F., Zheng, M., Beckwith, J. & Storz, G. Regulation of the OxyR transcription factor by hydrogen peroxide and the cellular thiol-disulfide status. Proc. Natl. Acad. Sci. USA 96, 6161-6165 (1999).
11. Hausladen, A., Privalle, C.T., Keng, T., DeAngeLo, J. & Stamler, J.S. Nitrosative stress: Activation of the transcription factor OxyR. Cell 86, 719-729 (1996).
12. Zorov, D.B., Filburn, C.R., Klotz, L.O., Zweier, J.L. & Sollott, S.J. Reactive oxygen species (ROS)-induced ROS release: A new phenomenon accompanying induction of the mitochondrial permeability transition in cardiac myocytes. J. Exp. Med. 192, 1001-1014 (2000).
13. Thomas, W.D., Zhang, X.D., Franco, A.V., Nguyen, T. & Hersey, P. TNF-related apoptosis-inducing ligand-induced apoptosis of melanoma is associated with changes in mitochondrial membrane potential and perinuclear clustering of mitochondria. J. Immunol. 165, 5612-5620 (2000).
14. Fulda, S., Meyer, E. & Debatin, K.M. Inhibition of TRAIL-induced apoptosis by Bcl-2 overexpression. Oncogene 21, 2283-2294 (2002).
15. Rokhlin, O.W., Guseva, N., Tagiyev, A., Knudson, C.M. & Cohen, M.B. Bcl-2 oncoprotein protects the human prostatic carcinoma cell line PC3 from TRAIL-mediated apoptosis. Oncogene 20, 2836-2843 (2001).
16. Walczak, H., Bouchon, A., Stahl, H. & Krammer, P.H. Tumor necrosis factor-related apoptosis-inducing ligand retains its apoptosis-inducing capacity on Bcl-2- or Bcl-xL-overexpressing chemotherapy-resistant tumor cells. Cancer Res. 60, 3051-3057 (2000).
17. Keogh, S.A., Walczak, H., Bouchier-Hayes, L. & Martin, S.J. Failure of Bcl-2 to block cytochrome c redistribution during TRAIL-induced apoptosis. FEES Lett. 471, 93-98 (2000).
18. Suzukawa, K. et al. Nerve growth factor-induced neuronal differentiation requires generation of Rac1-regulated reactive oxygen species. J. Biol. Chem. 275, 13175-13178 (2000).
19. Kim, S.O. et al. OxyR: A molecular code for redox-related signaling. Cell 109, 383-396 (2002).
20. Mukhopadhyay, P., Zheng, M., Bedzyk, L.A., LaRossa, R.A. & Storz, G. Prominent roles of the NorR and Fur regulators in the Escherichia coli transcriptional response to reactive nitrogen species. Proc. Natl. Acad. Sci. USA 101, 745-750 (2004).
21. Lee, M.W. et al. The involvement of reactive oxygen species (ROS) and p38 mitogen-activated protein (MAP) kinase in TRAIL/Apo2L-induced apoptosis. FEBS Lett. 512, 313-318 (2002).
22. Dooley, C.T. et al. Imaging dynamic redox changes in mammalian cells with green fluorescent protein indicators. J. Biol. Chem. 279, 22284-22293 (2004).
23. Hanson, G.T. et al. Investigating mitochondrial redox potential with redox-sensitive green fluorescent protein indicators. J. Biol. Chem. 279, 13044-13053 (2004).