Enzyme synthesis and activity assay in microfluidic droplets on a chip

Engineering in Life Sciences

Volumn 11, Issue 2, 2011, Pages 157-164

  Wu, Nan ^a,b   Courtois, Fabienne ^c   Surjadi, Regina ^a   Oakeshott, John ^a   Peat, Thomas S ^a   Easton, Christopher J ^b   Abell, Christopher ^c   Zhu, Yonggang ^a  

^a CSIRO   (Australia)

^b AUSTRALIAN NATIONAL UNIVERSITY   (Australia)

^c UNIVERSITY OF CAMBRIDGE   (United Kingdom)

Author keywords

Droplet based bioreactors; Emulsion; High throughput; Microfluidics; OpdA expression

Indexed keywords

ACTIVITY ASSAYS; AGROBACTERIUM RADIOBACTER; BIOCHEMICAL REACTIONS; DIRECTED EVOLUTION; DROPLET SYSTEM; DROPLET-BASED BIOREACTORS; DRUG DISCOVERY; ENZYME SYNTHESIS; GROWING DEMAND; HIGH-THROUGHPUT; HIGH-THROUGHPUT MEASUREMENTS; IN-VITRO; MICRO FLUIDIC SYSTEM; MODEL SYSTEM; OPDA EXPRESSION; ORGANOPHOSPHORUS HYDROLASE; SCREENING METHODS; WATER-IN-OIL;

BIOCONVERSION; BIOLOGICAL WATER TREATMENT; BIOREACTORS; DROPS; EMULSIFICATION; ENZYME ACTIVITY; ENZYMES; POLYMETHYL METHACRYLATES; SUBSTRATES; THROUGHPUT; TRANSCRIPTION;

MICROFLUIDICS;

ORGANOPHOSPHORUS COMPOUND; WATER;

BACTERIUM; BIOREACTOR; DROPLET; EMULSION; ENZYME ACTIVITY; FLUID FLOW; GENE EXPRESSION; GENETIC STRUCTURE; POLYMER; TECHNOLOGICAL DEVELOPMENT;

ARTICLE; BIOREACTOR; ENZYME ACTIVITY; ENZYME SYNTHESIS; FLUORESCENCE; GENETIC TRANSCRIPTION; HIGH THROUGHPUT SCREENING; IN VITRO STUDY; MICROCHIP ANALYSIS; MICROFLUIDICS; NONHUMAN; PROTEIN EXPRESSION; RHIZOBIUM RADIOBACTER; RNA TRANSLATION;

AGROBACTERIUM TUMEFACIENS;

EID: 79955420236     PISSN: 16180240     EISSN: 16182863     Source Type: Journal    
DOI: 10.1002/elsc.201000043     Document Type: Article

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