Miniaturized flow-through PCR with different template types in a silicon chip thermocycler

Lab on a Chip

Volumn 1, Issue 1, 2001, Pages 42-49

  Schneegaß, Ivonne ^a   Bräutigam, Reiner ^b   Köhler, Johann Michael ^a  

^a LEIBNIZ INSTITUTE OF PHOTONIC TECHNOLOGY   (Germany)

^b Clondiag Chip Technologies GmbH   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

GLASS; GLUTARALDEHYDE 3 PHOSPHATE DEHYDROGENASE; NUCLEIC ACID; OXIDOREDUCTASE; SILICON; UNCLASSIFIED DRUG; ZINC FINGER PROTEIN;

ARTICLE; BASE PAIRING; CHEMICAL COMPOSITION; DNA TEMPLATE; ELECTRONICS; ENERGY CONSUMPTION; FILM; GEL ELECTROPHORESIS; GENE AMPLIFICATION; HEATING; HOUSEKEEPING GENE; LIQUID; ONCOGENE MYC; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SURFACE PROPERTY; THERMOREGULATION;

EID: 10744227060     PISSN: 14730197     EISSN: None     Source Type: Journal    
DOI: 10.1039/b103846j     Document Type: Article

References (29)

1. K. B. Mullis, F. Ferré and R. A. Gibbs, The polymerase chain reaction, Birkhäuser, Boston. 1994.
2. G. R. Taylor and P. Robinson, Curr. Opin. Biotechnol., 1998, 9, 35.
3. D. Whitcombe, C. R. Newton and S. Little, Curr. Opin. Biotechnol., 1998, 9, 602.
4. M. A. Northrup, B. Benett, D. Hadley, P. Stratton and P. Landre, Advantages afforded by miniatuization and integration of DNA analysis instrumentation. 1st International Conference on Microreaction Technology, ed. W. Ehrfeld, Frankfurt a.M., 1997, pp. 278-287.
5. S. Poser, T. Schulz, U. Dillner, V. Baier and J. M. Köhler, Chip elements for fast thermocycling. Eurosensors X - The 10th European Conference on Solid-State Transducers, Leuven, Belgium, 1996, 4, pp. 1197-1199.
6. S. Poser, T. Schulz, U. Dillner, V. Baier, J. M. Köhler, D. Schimkat, G. Mayer and A. Siebert, Sens. Actuators A, 1997, 62, 672.
7. S. Poser, T. Schulz, U. Dillner, V. Baier, J. M. Köhler, G. Mayer, A. Siebert and D. Schimkat, Temperature controlled chip reactor for rapid PCR. 1st International Conference on Microreaction Technology, ed. W. Ehrfeld, Frankfurt a.M., 1997, pp. 294-301.
8. C. H. Mastrangelo, M. A. Burns and D. T. Burke, IEEE, 1998, 86, 1769.
9. M. Freemantle, Chem. Eng. Newsring, 1999, 77, 27.
10. J. Cheng, M. A. Shoffner, G. E. Hvichia, L. J. Kricka and P. Wilding, Nucleic Acids Res., 1996, 24, 380.
11. A. T. Woolley, D. Hadley, P. Landre, A. J. Demello, R. A. Mathies and M. A. Northrup, Anal. Chem., 1996, 68, 4081.
12. J. H. Daniel, S. Iqbal, R. B. Milligton, D. F. Moore, C. R. Lowe, D. L. Leslie, M. A. Lee and M. A. Pearce, Sens. Actuators A, 1998, 71, 81.
13. M. A. Northrup, C. Fonzalez, D. Hadley, R. F. Hills, P. Landre, S. Lehew, R. Saiki, J. J. Sninsky, R. Watson and R. J. Watson, A MEMS-based miniaturized DNA analysis system. TRANSDUCER '95. EURO-SENSOR IX - The 8th International Conference on Solid-State Sensors and Actuators and Eurosensors IX, Stockholm, Sweden, 1995, 193, pp. 764-767.
14. P. Wilding, M. A. Shoffner and L. J. Kricka, Clin. Chem., 1994, 40, 1815.
15. M. U. Kopp, A. J. DeMello and A. Manz, Science, 1998, 280, 1046. ** This paper was the first to refer to the successful rapid amplification in a continuous flow chip thermocycler.
16. J. M. Köhler, U. Dillner, A. Mokansky, S. Poser and T. Schulz, Micro channel reactors for fast thermocycling. 2nd International Conference on Microreaction Technology, ed. W. Ehrfeld, New Orleans, LA, USA, 1998. pp. 241-247.
17. S. Poser, R. Ehricht, T. Schulz, S. Uebel, U. Dillner and J. M. Köhler, Rapid PCR in flow-through Si chip thermocyclers. 3th International Conference on Microreaction Technology, ed. W. Ehrfeld, Frankfurt a.M., 1999, pp. 294-301. * This paper describes the different designs of continuous flow chip thermocyclers, presenting results of FEM simulations and demonstrating successful DNA amplification in these devices.
18. M. A. Shoffner, J. Cheng, G. E. Hvichia, L. J. Kricka and P. Wilding, Nucleic Acids Res., 1996, 24, 375. * This is one of the first papers in which the authors descibe in detail basic methods for the treatment of silicon-glass chips, in order to achieve a surface passivation for the PCR compatibility.
19. R. Ehricht, T. Ellinger and J. S. McCaskill, Eur. J. Biochem., 1997, 243, 358.
20. T. Ellinger, R. Ehricht and J. S. McCaskill, Chem. Biol., 1998, 5, 729.
21. M. J. Apostolakos, W. H. Schuermann, M. W. Framton, M. J. Utell and J. C. Willey, Anal. Biochem., 1993, 213, 277.
22. K. Peukert, P. Staller, A. Schneider, G. Carmichael, F. Hanel and M. Eilers, EMBO J., 1997, 16, 5672.
23. J. Sambrook, E. F. Fritsch and T. Maniatis, Molecular cloning: a laboratory manual, Cold Spring Habor, NY, 1989.
24. A. I. K. Lao, T. M. H. Lee, M. C. Carles and I.-M. Hsing, Thermal management and surface passivation of a miniaturized PCR device for traditional chinese medicine. Micro Total Analysis Systems 2000, ed. A. van den Berg, Enschede, The Nederlands, 2000, pp. 139-142.
25. C. T. Wittwer, G. B. Reed and K. M. Ririe, The polymerase chain reaction, ed. K. B. Mullis, F. Ferré and R. A. Gibbs, Birkhauser, Boston, 1994, pp. 174-181.
26. E. T. Lagally, P. C. Simpson and R. A. Mathies, Sens. Actuators B, 2000, 63, 138.
27. M. A. Burns, B. N. Johnson, S. N. Brahmasandra, K. Handique, J. R. Webster, M. Krishnan, T. S. Sammarco, P. M. Man, D. Jones, D. Heldsinger, C. H. Mastrangelo and D. T. Burke, Science, 1998, 282, 484.
28. C. L. Colyer, T. Tang, N. Chiem and D. J. Harrison, Electrophoresis, 1997, 18, 1733.
29. D. J. Harrison, K. Fluri, K. Seiler, Z. Fan, C. S. Effenhauser and A. Manz, Science, 1993, 261, 895.