Modulating the pH-activity profile of cellulase A from Cellulomonas fimi by replacement of surface residues

Protein Engineering, Design and Selection

Volumn 24, Issue 5, 2011, Pages 429-437

  Cockburn, Darrell W ^a,b   Clarke, Anthony J ^a  

^a UNIVERSITY OF GUELPH   (Canada)

^b TECHNICAL UNIVERSITY OF DENMARK   (Denmark)

Author keywords

cellulase; Cellulomonas fimi; endoglucanase; pH activity; protein engineering

Indexed keywords

ACIDIC CONDITIONS; ACIDIC PH; ACTIVITY PROFILE; ADDITIVE EFFECTS; ASSOCIATED COSTS; CELLULASE; CELLULOLYTIC ENZYME; CELLULOMONAS; CELLULOMONAS FIMI; CELLULOSE CONTENT; CHARGED GROUPS; DOUBLE MUTATION; ENDOGLUCANASES; GLYCOSIDE HYDROLASES; INDUSTRIAL PROCESSS; MUTANT ENZYMES; NATURAL AMINO ACIDS; NEUTRAL PH; PH OPTIMA; PLANT MATERIAL; PROTEIN ENGINEERING; SEQUENCE ALIGNMENTS; STEAM EXPLOSION; STRONG ACIDS; SURFACE CHARGE PROPERTIES; SURFACE RESIDUES; THREE-DIMENSIONAL STRUCTURE; VALUE ADDED PRODUCTS; WILD-TYPE LEVELS;

AMINO ACIDS; BAST FIBERS; BIOCHEMICAL ENGINEERING; CATALYSTS; CELLULOSIC ETHANOL; ENZYMES; ETHANOL; GENETIC ENGINEERING; HYDROLASES; SUGARS; SURFACES; THREE DIMENSIONAL;

ENZYME ACTIVITY;

AMINO ACID DERIVATIVE; ASPARAGINE; ASPARTIC ACID; BACTERIAL ENZYME; CELLULASE A; CYSTEINE; CYSTEINE SULFINIC ACID; GLUTAMINE; GLYCOSIDASE; RECOMBINANT PROTEIN; SERINE; TYROSINE; UNCLASSIFIED DRUG;

AMINO ACID COMPOSITION; AMINO ACID SUBSTITUTION; ARTICLE; BACTERIAL STRAIN; BACTERIUM; CELLULOMONAS; CELLULOMONAS FIMI; CIRCULAR DICHROISM; CONTROLLED STUDY; COPRINOPSIS CINEREA; ENZYME ACTIVITY; ENZYME ENGINEERING; ENZYME LOCALIZATION; ENZYME MECHANISM; ENZYME METABOLISM; ENZYME STABILITY; ENZYME STRUCTURE; ESCHERICHIA COLI; HUMICOLA INSOLENS; HYPOCREA JECORINA; MODEL; MUTATION; MYCOBACTERIUM TUBERCULOSIS; NONHUMAN; PH; PLASMID; PRIORITY JOURNAL; SEQUENCE ALIGNMENT; SITE DIRECTED MUTAGENESIS; THERMOBIFIDA FUSCA; WILD TYPE;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; CATALYTIC DOMAIN; CELLULASE; CELLULOMONAS; GLYCOSIDE HYDROLASES; HYDROGEN-ION CONCENTRATION; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTATION; SEQUENCE ALIGNMENT;

AMINO ACIDS; BAST FIBERS; CATALYSTS; CELLULASE; ENDO ENZYMES; ETHANOL; GLUCANASE; PH; SUGARS; SURFACES; THREE DIMENSIONAL DESIGN;

CELLULOMONAS FIMI;

EID: 79955370570     PISSN: 17410126     EISSN: 17410134     Source Type: Journal    
DOI: 10.1093/protein/gzr004     Document Type: Article

References (63)

1. André,G., Kanchanawong,P., Palma,R., Cho,H., Deng,X., Irwin,D., Himmel,M.E., Wilson,D.B. and Brady,J.W. (2003) Protein Eng., 16, 125-134.
2. Bas,D.C., Rogers,D.M. and Jensen,J.H. (2008) Proteins, 73, 765-783.
3. Bauer,S., Vasu,P., Persson,S., Mort,A.J. and Somerville,C.R. (2006) Proc. Natl. Acad. Sci. USA, 103, 11417-11422.
4. Becker,D., Braet,C., Brumer,H.E., et al. (2001) Biochem. J., 356, 9-30.
5. Clarke,A.J. (1997) Biodegradation of Cellulose. Enzymology and Biotechnology. CRC Press, Boca Raton, FL.
6. Chow,C.M., Yagüe,E., Raguz,S., Wood,D.A. and Thurston,C.F. (1994) Appl. Environ. Microbiol., 60, 2779-2785.
7. Cockburn,D.W., Vandenende, C. and Clarke, A.J. (2010) Biochemistry, 49,2042-2050.
8. Damude,H.G., Ferro,V., Withers,S.G. and Warren,R.A. (1996) Biochem. J., 315(Pt 2), 467-472.
9. Damude,H.G., Withers,S.G., Kilburn,D.G., Miller,R.C., Jr. and Warren,R.A. (1995) Biochemistry, 34, 2220-2224.
10. Ding,S., Adney,W., Vinzant,T., Himmel,M. and Decker,S. (2001) Thermal tolerant cellulose from Acidothermus cellulolyticus. International patent WO/2003/012109.
11. Ellman,G. and Lysko,H. (1979) Anal. Biochem., 93, 98-102.
12. Fernández-Abalos,J.M., Sánchez,P., Coll,P.M., Villanueva,J.R., Pérez,P. and Santamaría,R.I. (1992) J. Bacteriol., 174, 6368-6376.
13. Fierobe,H.P., Clarke,A.J., Tull,D. and Svensson,B. (1998a) Biochemistry, 37, 3753-3759.
14. Fierobe,H.P., Mirgorodskaya,E., McGuire,K.A., Roepstorff,P., Svensson,B. and Clarke,A.J. (1998b) Biochemistry, 37, 3743-3752.
15. Fraczkiwicz,R. and Braun,W. (1998) J. Comp. Chem., 19, 319-333.
16. Gilkes,N.R., Claeyssens,M., Aebersold,R., Henrissat,B., Meinke,A., Morrison,H.D., Kilburn,D.G., Warren,R.A.J. and Miller,R.C., Jr. (1991) Eur. J. Biochem., 202, 367-377.
17. Gordon,J.C., Myers,J.B., Folta,T., Shoja,V., Heath,L.S. and Onufriev,A. (2005) Nucleic Acids Res., 33, W368-71.
18. Grimsley,G.R., Shaw,K.L., Fee,L.R., Alston,R.W., Huyghues-Despointes,B.M. , Thurlkill,R.L., Scholtz,J.M. and Pace,C.N. (1999) Protein Sci., 8, 1843-1849.
19. Guex,N. and Peitsch,M.C. (1997) Electrophoresis, 18, 2714-2723.
20. Haque,I., Singh,R., Moosavi-Movahedi,A.A. and Ahmad,F. (2005) Biophys. Chem., 117, 1-12.
21. Heinzelman,P., Snow,C.D., Wu,I., Nguyen,C., Villalobos,A., Govindarajan,S., Minshull,J. and Arnold,F.H. (2009). Proc. Natl. Acad. Sci. USA, 106, 5610-5615.
22. Holman,C.M. and Benisek,W.F. (1994) Biochemistry, 33, 2672-2681.
23. Ibarra-Molero,B., Loladze,V.V., Makhatadze,G.I. and Sanchez-Ruiz,J.M. (1999) Biochemistry, 38, 8138-8149.
24. Kim,T., Mullaney,E.J., Porres,J.M., et al. (2006) Appl. Environ. Microbiol., 72, 4397-403.
25. Koivula,A., Reinikainen,T., Ruohonen,L., et al. (1996) Protein Eng., 9, 691-699.
26. Koivula,A., Ruohonen,L., Wohlfahrt,G., et al. (2002) J. Am. Chem. Soc., 124, 10015-10024.
27. Larkin,M.A., Blackshields,G. and Brown,N.P., et al. (2007) Bioinformatics, 23, 2947-2948.
28. Larsson,A.M., Bergfors,T., Dultz,E., Irwin,D.C., Roos,A., Driguez,H., Wilson,D.B. and Jones,T.A. (2005) Biochemistry, 44, 12915-12922.
29. Liu,S., Li,D.E.S. and Zhang,Y. (2005) Sheng Wu Gong Cheng Xue Bao, 21, 892-899.
30. Loladze,V.V., Ibarra-Molero,B., Sanchez-Ruiz,J.M. and Makhatadze,G.I. (1999) Biochemistry, 38, 16419-16423.
31. Makde,R.D., Dikshit,K. and Kumar,V. (2006) Biomol. Eng., 23, 247-51.
32. Meinke,A., Gilkes,N.R., Kwan,E., Kilburn,D.G., Warren,R.A. and Miller,R.C., Jr. (1994) Mol. Microbiol., 12, 413-422.
33. Nelson,N. (1944) J. Biol. Chem., 153, 375-380.
34. Nielsen,J.E., Beier,L., Otzen,D., Borchert,T.V., Frantzen,H.B., Andersen,K.V. and Svendsen,A. (1999) Eur. J. Biochem., 34, 889-824.
35. Nielsen,J.E., Borchert,T.V. and Vriend,G. (2001) Prot. Eng., 114, 505-512.
36. Palmieri,F., Stipani,I. and Iacobazzi,V. (1979) Biochim. Biophys. Acta, 555, 531-546.
37. Park,C., Kawaguchi,T., Sumitani,J., Takada,G., Izumori,K. and Arai,M. (2005) J. Biosci. Bioeng., 99, 434-436.
38. Roy,A., Kucukural,A.,Zhang,Y., (2010) Nat. Protoc.,5,725-738.
39. Runquist,J.A. and Miziorko,H.M. (2002) Arch. Biochem. Biophys., 405, 178-184.
40. Russell,A.J. and Fersht,A.R. (1987) Nature, 328, 496-500.
41. Shen,H., Schmuck,M., Pilz,I., Gilkes,N.R., Kilburn,D.G., Miller,R.C., Jr. and Warren,R.A.J. (1991) J. Biol. Chem., 266, 11335-11340.
42. Schülein,M. (1997) J. Biotechnol., 57, 71-81.
43. Somogyi,M. (1952) J. Biol. Chem., 195, 19-23.
44. Spector,S., Wang,M., Carp,S.A., Robblee,J., Hendsch,Z.S., Fairman,R., Tidor,B. and Raleigh,D.P. (2000) Biochemistry, 39, 872-879.
45. Spezio,M., Wilson,D.B. and Karplus,P.A. (1993) Biochemistry, 32, 9906-9916.
46. Sternberg,M.J.E., Hayes,F.R.F., Russell,A.J., Thomas,P.G. and Fersht,A.R. (1987) Nature, 330, 86-88.
47. Thomas,P.G., Russell,A.J. and Fersht,A.R. (1985) Nature, 318, 375-376.
48. Tsong,T.Y., Hearn,R.P., Wrathall,D.P. and Sturtevant,J.M. (1970) Biochemistry, 9, 2666-2677.
49. Tynan-connolly,B.M. and Nielsen,J.E. (2007) Protein Sci., 16, 239-249.
50. Tuohy,M.G., Walsh,D.J., Murray,P.G., Claeyssens,M., Cuffe,M.M., Savage,A.V. and Coughlan,M.P. (2002) Biochim. Biophys. Acta, 1596, 366-380.
51. Urry,D.W., Gowda,D.C., Peng,S., Parker,T.M., Jing,N. and Harris,R.D. (1994) Biopolymers, 34, 889-896.
52. Varrot,A., Leydier,S., Pell,G., Macdonald,J.M., Stick,R.V., Henrissat,B., Gilbert,H.J. and Davies,G.J. (2005) J. Biol. Chem., 280, 20181-20184.
53. Voss,J., Sun,J., Venkatesan,P. and Kaback,H.R. (1998) Biochemistry, 37, 8191-8196.
54. Vuong,T.V. and Wilson,D.B. (2009a) FEBS J., 276, 3837-3845.
55. Vuong,T.V. and Wilson,D.B. (2009b) Appl. Environ. Microbiol., 75, 6655-6661.
56. Wang,T., Liu,X., Yu,Q., Zhang,X., Qu,Y., Gao,P. and Wang,T. (2005) Biomol. Eng., 22, 89-94.
57. Wolfgang,D.E. and Wilson,D.B. (1999) Biochemistry, 38, 9746-9751.
58. Wohlfahrt,G., Pellikka,T., Boer,H., Teeri,T.T. and Koivula,A. (2003) Biochemistry, 42, 10095-10103.
59. Wong,W.K., Gerhard,B., Guo,Z.M., Kilburn,D.G., WarrenA.J. and MillerR.C. Jr.(1986) Gene,44,315-324.
60. Zhang,S. and Wilson,D.B. (1997) J. Biotechnol., 57, 101-113.
61. Zhang,S., Barr,B.K. and Wilson,D.B. (2000a) Eur. J. Biochem., 267, 244-252.
62. Zhang,S., Irwin,D.C. and Wilson,D.B. (2000b) Eur. J. Biochem., 267, 3101-3115.
63. Zheng,L., Baumann,U. and Reymond,J. (2004) Nucleic Acids Res., 32, e115.