Probing pH-dependent functional elements in proteins: Modification of carboxylic acid pairs in Trichoderma reesei cellobiohydrolase ce16A

Biochemistry

Volumn 42, Issue 34, 2003, Pages 10095-10103

  Wohlfahrt, Gerd ^a   Pellikka, Tarmo ^b   Boer, Harry ^b   Teeri, Tuula T ^c   Koivula, Anu ^b  

^a ORION CORPORATION   (Finland)

^b VTT TECHNICAL RESEARCH CENTRE OF FINLAND   (Finland)

^c ROYAL INSTITUTE OF TECHNOLOGY   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

CARBOXYLIC ACIDS; CONFORMATIONS; ENZYMES; HYDROGEN BONDS; PH; PROTONS;

MUTANTS;

PROTEINS;

CARBOXYLIC ACID; CELLOTETRAOSE; CELLULOSE 1,4 BETA CELLOBIOSIDASE; TRYPTOPHAN; UNCLASSIFIED DRUG;

ARTICLE; CATALYSIS; CHEMICAL MODIFICATION; CIRCULAR DICHROISM; ENZYME ACTIVE SITE; ENZYME STABILITY; FLUORESCENCE; GEOMETRY; HALF LIFE TIME; HYDROGEN BOND; NONHUMAN; PH; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN FOLDING; TEMPERATURE; TRICHODERMA REESEI;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ASPARTIC ACID; BINDING SITES; CATALYSIS; CELLULASE; CELLULOSE; CELLULOSE 1,4-BETA-CELLOBIOSIDASE; CIRCULAR DICHROISM; ENZYME STABILITY; GLUTAMIC ACID; HYDROGEN-ION CONCENTRATION; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PROTEIN DENATURATION; RECOMBINANT PROTEINS; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY, AMINO ACID; SPECTROMETRY, FLUORESCENCE; TETROSES; TRICHODERMA;

BACTERIA (MICROORGANISMS); HYPOCREA JECORINA; TRICHODERMA;

EID: 0042324606     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi034954o     Document Type: Article

References (46)

1. Sanchez-Ruiz, J. M., and Makhatadze, G. I. (2001) Trends Biotechnol. 19, 132-135.
2. Sawyer, L., and James, M. N. G. (1982) Nature 295, 79-80.
3. Berman, H. M., Westbrook, J., Feng, Z., Gilliland, G., Bhat, T. N., Weissig, H., Shindyalov, I. N., and Bourne, P. E. (2000) Nucleic Acids Res. 28, 235-242.
4. Flocco, M. M., and Mowbray, S. L. (1995) J. Mol. Biol. 254, 96-105.
5. Mortensen, U. H., and Breddam, K. (1994) Protein Sci. 3, 838-842.
6. Cleland, W. W., Frey, P. A., and Gerlt, J. A. (1998) J. Biol. Chem. 273, 25529-25532.
7. Teeri, T. T. (1997) Trends Biotech. 15, 160-167.
8. Rouvinen, J., Bergfors, T., Teeri, T. T., Knowles, J. K., and Jones, T. A. (1990) Science 249, 380-386.
9. Divne, C., Ståhlberg, J., Reinikainen, T., Ruohonen, L., Pettersson, G., Knowles, J. K. C., Teeri, T. T., and Jones, T. A. (1994) Science 265, 524-528.
10. Barr, B. K., Wolfgang, D. E., Piens, K., Claeyssens, M., and Wilson, D. B. (1998) Biochemistry 37, 9220-9229.
11. Zou, J., Kleywegt, G. J., Stahlberg, J., Driguez, H., Nednckx, W., Claeyssens, M., Koivula, A., Teeri, T. T., and Jones, T. A. (1999) Structure Fold. Des. 7, 1035-1045.
12. Koivula, A., Ruohonen, L., Wohlfahrt, G., Reinikainen, T., Teeri, T. T., Piens, K., Claeyssens, M., Weber, B.,Vasella, A., Becker, D., Sinnott, M. L., Zou, J., Kleywegt, G., Szardenings, M., Ståhlberg, J., and Jones, T. A. (2002) J. Am. Chem. Soc. 124, 10015-10024.
13. Coutinho, P. M., and Henrissat, B. (1999) in Recent Advances in Carbohydrate Bioengineering (Gilbert, H. J., Davies, G., Henrissat, B., and Svensson, B., Eds.) pp 3-12, The Royal Society of Chemistry, Cambridge, UK.
14. Koivula, A., Reinikainen, T., Ruohonen, L., Valkeajärvi, A., Claeyssens, M., Teleman, O., Kleywegt, G., Szardenings, M., Rouvinen, J., Jones, T. A., and Teeri, T. T. (1996) Protein Eng. 9, 691-699.
15. Mach, R. L., Schindler, M., and Kubicek, C. P. (1994) Curr. Genet. 6, 567-570.
16. Ståhlberg, J., Divne, C., Koivula, A., Piens, K., Claeyssens, M., Teeri, T. T., and Jones, T. A. (1996) J. Mol. Biol. 264, 337-349.
17. Srisodsuk, M., Reinikainen, T., Penttilä, M., and Teeri, T. T. (1993) J. Biol. Chem. 268, 20756-20761.
18. Reinikainen, T., Henrikson, K., Siika-aho, M., Teleman, O., and Poutanen, K. (1994) Enzyme Microbiol. Technol. 17, 888-892.
19. Tomme, P., McRae, S., Wood, T. M., and Claeyssens, M. (1988) Methods Enzymol. 160, 187-192.
20. Laemmli, U. K. (1970) Nature 227, 680-685.
21. Towbin, H., Staehelin, T., and Gordon, J. (1979) Proc. Natl. Acad. Sci. U.S.A. 76, 4350-4354.
22. Koivula, A., Lappalainen, A., Virtanen, S., Mäntylä, A. L., Suominen, P., and Teeri, T. T. (1996) Protein Exp. Purif. 8, 391-400.
23. Aho, S., Olkkonen, V., Jalava, T., Paloheimo, M., Bühler, R., Niku-Paavola, M., Bamford, D. H., and Korhola, M. (1991) Eur. J. Biochem. 200, 643-649.
24. Teleman, A., Koivula, A., Reinikainen, T., Valkeajärvi, A., Teeri, T. T., Drakenberg, T., and Teleman, O. (1995) Eur. J. Biochem. 231, 250-258.
25. Gilkes, N. R., Jervis, E., Henrissat, B., Tekant, B., Miller, R., Jr., Warren, A., and Kilburn, D. G. (1992) J. Biol. Chem. 267, 6743-6749.
26. Boer, H., Teeri, T. T., and Koivula, A. (2000) Biotech. Bioeng. 69, 486-494.
27. Isogai, A., and Atalla, R. H. (1990) J. Polym. Sci., Part A: Polym. Chem. 29, 113-119.
28. Eftink, M. R. (1995) Methods Enzymol. 259, 487-512.
29. Pace, C. N. (1986) Methods Enzymol. 131, 266-280.
30. Nozaki, Y. (1972) Methods Enzymol. 26, 43-50.
31. Alder, A. J., Greenfield, N. J., and Fasman, G. D. (1973) Methods Enzymol. 27, 675-735.
32. Pagès, S., Kester, H. C., Visser, J., and Benen, J. A. (2001) J. Biol. Chem. 276, 33652-33656.
33. Kennedy, J. F., Cabalda, V. M., and White, C. A. (1988) TIBTECH 6, 184-189.
34. Kashyap, D. R., Vohra, P. K., Chopra, S., and Tewari, R. (2001) Biores. Technol. 77, 215-227.
35. Russell, R. B., and Barton, G. J. (1992) Proteins 14, 309-323.
36. Thompson, J. D., Higgins, D. G., and Gibson, T. J. (1994) Nucleic Acids Res. 22, 4673-4680.
37. Mizuguchi, K., Deane, C. M., Blundell, T. L., Johnson, M. S., and Overington, J. P. (1998) Bioinformatics 14, 617-623.
38. Christakopoulos, P., Kekos, D., Macris, B. J., Claeyssens, M., and Bhat, M. K. (1995) Arch. Biochem. Biophys. 316, 428-433
39. Chow, C., Yague, E., Raguz, S., Wood, D. A., and Thurston, C. F. (1994) Appl. Environ. Microbiol. 60, 2779-2785.
40. Denman, S., Xue, G. P., and Patel, B. (1996) Appl. Environ. Microbiol. 62, 1889-1896.
41. Liu, J., Tsai, C., Liu, J., Cheng, K., and Cheng, C. (2001) Enzyme Microbiol. Technol. 28, 582-589.
42. Li, X. L., Chen, H., and Ljungdahl, L. G. (1997) Appl. Environ. Microbiol. 63, 4721-4728.
43. Schulein, M. (1997) J. Biotechnol. 57, 71-81.
44. Wolfgang, D. E., and Wilson, D. B. (1999) Biochemistry 38, 9746-9751.
45. Stålbrand, H., Mansfield, S. D., Saddler, J. N., Kilburn, D. G., Warren, R. A., and Gilkes, N. R. (1998) Appl. Environ. Microbiol. 64, 2374-2379.
46. Zhang, S., Lao, G., and Wilson, D. B. (1995) Biochemistry 34, 3386-3395.