Effects of noncatalytic residue mutations on substrate specificity and ligand binding of Thermobifida fusca endocellulase Cel6A

European Journal of Biochemistry

Volumn 267, Issue 1, 2000, Pages 244-252

  Zhang, Sheng ^a,b   Barr, Brian K ^a,c   Wilson, David B ^a  

^a Department of Population Medicine and Diagnostic Sciences   (United States)

^b Adv BioAnalytical Services Inc   (United States)

^c LOYOLA UNIVERSITY MARYLAND   (United States)

Author keywords

Endocellulase; Ligand binding; Site directed mutagenesis; Substrate specificity; Thermobifida fusca

Indexed keywords

BACTERIAL ENZYME; CARBOXYMETHYLCELLULOSE; CELLULASE; CELLULOSE; OLIGOSACCHARIDE; POLYSACCHARIDE;

ARTICLE; CONTROLLED STUDY; DISSOCIATION CONSTANT; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME BINDING; ENZYME CONFORMATION; ENZYME PURIFICATION; ENZYME SPECIFICITY; HYDROLYSIS; LIGAND BINDING; NONHUMAN; PH; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS; THERMOMONOSPORA;

ACTINOMYCETALES; AMINO ACID SUBSTITUTION; BINDING SITES; CATALYTIC DOMAIN; CELLULASE; CELLULOSE; CELLULOSE 1,4-BETA-CELLOBIOSIDASE; CONSERVED SEQUENCE; DIMERIZATION; ELECTROSTATICS; ENZYME STABILITY; ESCHERICHIA COLI; HYDROGEN BONDING; HYDROGEN-ION CONCENTRATION; KINETICS; LIGANDS; MODELS, MOLECULAR; MUTATION; OLIGOSACCHARIDES; RECOMBINANT PROTEINS; REDUCING AGENTS; SOLUBILITY; SUBSTRATE SPECIFICITY;

EID: 0033981570     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.2000.00988.x     Document Type: Article

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