Surface residue mutations which change the substrate specificity of Thermomonospora fusca endoglucanase E2

Journal of Biotechnology

Volumn 57, Issue 1-3, 1997, Pages 101-113

  Zhang, Sheng ^a   Wilson, David B ^a  

^a Department of Population Medicine and Diagnostic Sciences   (United States)

Author keywords

Conformational change; Endoglucanase; Site directed mutagenesis; Substrate specificity; Surface residues; Thermomonospora fusca

Indexed keywords

CATALYSIS; CATALYST ACTIVITY; CELLULOSE; CONFORMATIONS; CRYSTAL STRUCTURE; HYDROLYSIS; MUTAGENESIS; SUBSTRATES; SURFACE PROPERTIES; X RAY CRYSTALLOGRAPHY;

ENDOGLUCANASE; SUBSTRATE SPECIFICITY; SURFACE RESIDUE MUTATIONS;

ENZYMES;

CELLULOSE; GLUCAN SYNTHASE;

ARTICLE; BACTERIUM; BIODEGRADATION; CONTROLLED STUDY; CRYSTALLOGRAPHY; ENZYME SPECIFICITY; HYDROLYSIS; MUTATION; NONHUMAN; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS; SURFACE PROPERTY;

CELLULASE; CIRCULAR DICHROISM; MUTAGENESIS, SITE-DIRECTED; OLIGOSACCHARIDES; STRUCTURE-ACTIVITY RELATIONSHIP; SUBSTRATE SPECIFICITY;

BACTERIA (MICROORGANISMS); THERMOBIFIDA FUSCA; THERMOMONOSPORA;

EID: 0343472021     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0168-1656(97)00093-X     Document Type: Article

References (34)

1. Adler, M., Scheraga, H.A., 1988. Structural studies of a folding intermediate of bovine pancreatic ribonuclease A by continuous recycled flow. Biochemistry 27 (7), 2471-2480.
2. Barr, B.K., Hsieh, Y.-L., Ganem, B., Wilson, D.B., 1996. Identification of two functionally different classes of exocellulases. Biochemistry 35, 586-592.
3. Bathwell, M., Daughhetee, S., Chau, G., Wilson, D.B., Walker, L., 1997. Binding capacities for thermomonospora fusca E3, E4 and E5, the E3 binding domain, and Trichoderma reesei CBHI on Avicel and bacterial microcrystalline cellulose. Bioresour. Technol. 60, 169-178.
4. Chung, C.T., Niemela, S.L., Miller, R.H., 1989. One-step preparation of competent Escherichia coli: transformation and storage of bacterial cells in the same solution. Proc. Natl. Acad. Sci. USA 86, 2172-2175.
5. Damude, H., Withers, S., Kilburn, D., Miller, R., Warren, R.A., 1995. Site-directed mutation of the putative catalytic residues of Endoglucanase CenA from Cellulomonas fimi. Biochemistry 34 (7), 2220-2224.
6. De Boeck, H., Matta, K.L., Claeyssens, M., Sharon, N., Loontiens, F.G., 1983. Binding of 4-Methylumbelliferyl β-D-galactosyl-(1-3)-N-acetyl-β-D-galactosaminide to peanut agglutinin. Characterization and application in substitution titrations. Eur. J. Biochem. 131, 453-460.
7. Fornwald, J.A., Donovan, M.J., Gerber, R., Keller, J., Taylor, D.P., Arcuri, E.J., Brawner, M.E., 1993. Soluble forms of the human T cell receptor CD4 are efficiently expressed by Streptomyces lividans. Bio/Technology 11, 1031-1036.
8. Ghangas, G.S., Wilson, D.B., 1988. Cloning of the Thermomonospora fusca endoglucanase E2 gene in Streptomyces lividans: affinity purification and functional domains of the cloned gene product. Appl. Environ. Microbiol. 54 (10), 2521-2526.
9. Gilkes, N.R., Henrissat, B., Kilburn, D.G., Miller, R.C. Jr., Warren, R.A.J., 1991. Domains in microbiol β-1,4-glycanases: sequence conservation, function, and enzyme families. Microbiol. Revs. 55 (2), 303-315.
10. Hanahan, D., 1983. Studies on transformation of Escherichia coli with plasmids. J. Mol. Biol. 166, 557-580.
11. Henrissat, B., Baroch, A., 1993. New families in the classification of glycosyl hydrolases based on amino acid sequence similarities. Biochem. J. 203, 781-788.
12. Ho, S.N., Hunt, H.D., Horton, R.M., Pullen, J.K., Pease, L.R., 1989. Site-directed mutagenesis by overlap extension using the polymerase chain reaction. Gene 77, 51-59.
13. Hopwood, D.A., Bibb, M.J., Chater, K.F., Kiersen, T., Bruton, C.J., Kieser, H.M., Lydiate, D.J., Smith, C.P., Ward, J.M., Schrempf, H., 1985. Genetic Manipulation of Streptomyces - A Laboratory Manual. The John Innes Foundation, Norwich, England.
14. Irwin, D.C., Spezio, M., Walker, L., Wilson, D.B., 1993. Activity studies of eight purified cellulases: specificity, synergism, and binding domain effects. Biotechnol. Bioeng. 42, 1002-1013.
15. Jung, E.D., Lao, G., Irwin, D., Barr, B.K., Benjamin, A., Wilson, D.B., 1993. DNA sequences and expression in Streptomyces lividans of an exoglucanase gene and an endoglucanase gene from Thermomonospora fusca. Appl. Environ. Microbiol. 59 (9), 3032-3043.
16. Koivula, A., Reinikainen, T., Ruohonen, L., Valkeajarvi, A., Claeyssens, M., Teleman, O., Kleywegt, G.J., Szardenings, M., Rouvinen, J., Jones, T.A., Teeri, T.T., 1996. The active site of Trichoderma reesei cellobiohydroase II: The role of Tyrosine-169. Protein Eng. 9 (8), 691-699.
17. Lao, G., Ghangas, G.S., Jung, E.D., Wilson, D.B., 1991. DNA sequences of three β-1,4-endoglucanase genes from Thermomonospora fusca. J. Bacteriol. 173 (11), 3397-3407.
18. McGinnis, K., Kroupis, C., Wilson, D.B., 1993. Dimerization of Thermomonospora fusca β-1,4-endoglucanase E2. Biochemistry 32 (32), 8146-8150.
19. Meinke, A., Damude, H.G., Tomme, P., Kawn, E., Kilburn, D.G., Miller, R.C. Jr., Warren, R.A.J., Gilkes, N.R., 1995. Enhancement of the endo-beta-1,4-glucanase activity of an exocellobiohydrolase by deletion of a surface loop. J. Biol. Chem. 270 (9), 4383-4386.
20. Miller, G.L., 1959. Use of dinitrosalicylic acid reagent for determination of reducing sugars. Anal. Chem. 31, 426-428.
21. Nidetzky, B., Steiner, W., Claeyssens, 1995. Synergistic interaction of cellulases from Trichoderma reesei during cellulose degradation. In: Saddler, J.N., Penner, M.H. (Eds.), Enzymatic Degradation of Insoluble Carbohydrate. American Chemical Society, Washington DC, USA, pp. 90-112.
22. Rouvinen, J., Bergfors, T., Teeri, T., Knowles, J.K.C., Jones, T.A., 1990. Three-dimensional structure of cellobiohydrolase II from Trichoderma reesei. Science 249, 380-386.
23. Ruohonen, L., Koivula, A., Reinikainen, T., Valkeajarvi, A., Teleman, A., Claeyssens, M., Szardenings, M., Jones, T.A., Teeri, T.T., 1993. Trichoderma reesei cellulases and other hydrolases. In: Suominen, P., Reinikainen, T., (Eds.), Enzyme structures, biochemistry, genetics and applications. Foundation for Biotechnical and Industrial Fermentation Research. Vol. 8, Helsinki, pp. 87-96.
24. Spezio, M., Wilson, D.B., Karplus, A., 1993. Crystal structure of the catalytic domain of a thermophilic endonuclease. Biochemistry 32 (38), 9906-9916.
25. Taylor, J.S., Teo, B., Wilson, D.B., Brady, J.W., 1995. Conformational modeling of substrate binding to endocellulase E2 from Thermomonospora fusca. Protein Eng. 8 (11), 1145-1152.
26. Teather, R.M., Wood, P.J., 1982. Use of Congo red polysaccharide interaction in enumeration and characterization of cellulolytic bacteria from the bovine rumen. Appl. Environ. Microbiol. 43, 777-780.
27. Tomme, P., Warren, R.A.J., Gilkers, N.R., 1995. Cellulose hydrolysis by bacteria and fungi. In: Poole, R.K. (Ed.), Advances in Microbial Physiology, Vol. 37. Academic press, London, pp. 2-81.
28. Van Tilbeurgh, H., Pettersson, G., Bhikabhai, R., De Boeck, H., Claeyssens, M., 1985. Studies of the cellulolytic system of Trichoderma reesei QM 9414. Reaction specificity and thermodynamics of interactions of small substrates and ligands with the 1,4-B-glucan cellobiohydrolase II. Eur. J. Biochem. 148, 329-334.
29. Wang, F., Kan, M., McKeehan, W.L., 1995. Multiple mutant cDNAs from one reaction mixture using asymmetric primers in PCR. BioTechniques 19 (4), 556-559.
30. Wilson, D.B., 1992. Biochemistry and genetics of actinomycete cellulases. Crit. Rev. Biotechnol. 12, 45-63.
31. Wilson, D.B., Spezio, M., Irwin, D., Karplus, A., Taylor, J., 1995. Comparison of enzymes catalyzing the hydrolysis of insoluble polysaccharides. In: Saddler J.N., Penner, M.H. (Eds.), Enzymatic Degradation of Insoluble Carbohydrates. American Chemical Society, Washington DC, USA, pp. 1-12.
32. Xu, G.-Y., Ong, E., Gilkes, N.R., Kilburn, D.G., Muhandiram, D.R., Harris-Brandts, M., Carver, J.P., Kay, L.E., Harvey, T.S., 1995. Solution structure of a cellulose-binding domain from Cellulomonas fimi by nuclear magnetic resonance spectroscopy. Biochemistry 34, 6993-7009.
33. Yang, J.T., Wu, C.S.C., Martinez, H.M., 1986. Calculation of protein conformation from circular dichroism. Methods Enzymol 130, 208-269.
34. Zhang, S., Lao, G., Wilson, D.B., 1995. Characterization of a bacterial exocellulase. Biochemistry 34 (10), 3386-3395.