High-throughput, high-level production of PS-tag-fused single-chain Fvs by microplate-based culture

Journal of Bioscience and Bioengineering

Volumn 111, Issue 5, 2011, Pages 569-573

  Kumada, Yoichi ^a   Takase, Yuta ^a   Sasaki, Eiju ^a   Kishimoto, Michimasa ^a  

^a KYOTO INSTITUTE OF TECHNOLOGY   (Japan)

Author keywords

Antibody microarray; High throughput production; Microplate based culture; PS tag; Single chain Fv; Solid phase refolding

Indexed keywords

ANTIBODY MICROARRAYS; HIGH-THROUGHPUT; MICROPLATE-BASED CULTURE; PS-TAG; SINGLE-CHAIN FV; SOLID-PHASE REFOLDING;

ANTIBODIES; CONTAMINATION; CULTIVATION; DIAGNOSIS; ESCHERICHIA COLI; MICROARRAYS; OXYGEN; PRODUCTIVITY;

THROUGHPUT;

POLYSTYRENE; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY;

ANTIBODY PRODUCTION; ARTICLE; CULTURE TECHNIQUE; ESCHERICHIA COLI; FLASK CULTURE; HYDROPHILICITY; MICROPLATE BASED CULTURE; NONHUMAN; OXYGEN TRANSPORT; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN INDUCTION;

ANIMALS; CULTURE TECHNIQUES; ENZYME-LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; MICE; POLYSTYRENES; PROTEIN FOLDING; SINGLE-CHAIN ANTIBODIES;

ESCHERICHIA COLI;

EID: 79954743989     PISSN: 13891723     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiosc.2011.01.008     Document Type: Article

References (20)

1. Roth A., Drummond D.C., Conrad F., Hayes M.E., Kirpotin D.B., Benz C.C., Marks J.D., Liu B. Anti-CD166 single chain antibody-mediated intracellular delivery of liposomal drugs to prostate cancer cells. Mol. Cancer Ther. 2007, 6:2737-2746.
2. Carrier A., Ducancel F.B., Settiawan N., Cattolico L., Maillere B., Leonetti M., Drevet P., Menez A., Boulain J.-C. Recombinant antibody-alkaline phosphatase conjugates for diagnosis of human IgGs: application to anti-HBsAg detection. J. Immunol. Meth. 1995, 181:177-186.
3. Gilchuk P.V., Volkov G.L. Immobilization of mouse single-chain antibodies for affinity chromatography using the cellulose-binding protein. Ukr. Biokhim. Zh. 2006, 78:160-163.
4. Borrebaeck C.A. Antibodies in diagnostics - from immunoassays to protein chips. Immunol. Today 2000, 21:379-382.
5. Shen Z., Yan H., Zhang Y., Mernaugh R.L., Zeng X. Single-chain fragment variable antibody piezoimmunosensors. Anal. Chem. 2008, 80:1910-1917.
6. Saerens D., Huang L., Bonroy K., Muyldermans S. Antibody fragments as probe in biosensor development. Sensors 2008, 8:4669-4689.
7. Verma R., Boleti E., George A.J.T. Antibody engineering: comparison of bacterial, yeast, insect and mammalian expression systems. J. Immunol. Meth. 1998, 216:165-181.
8. Browne S.M., Al-Rubeai M. Selection methods for high-producing mammalian cell lines. Trends Biotechnol. 2007, 25:425-432.
9. Chiarella P., Fazio V.M. Mouse monoclonal antibodies in biological research: strategies for high-throughput production. Biotechnol. Lett. 2008, 30:1303-1310.
10. Skerra A. Bacterial expression of immunoglobulin fragments. Curr. Opin. Immunol. 1993, 5:256-262.
11. Tsumoto K., Shinoki K., Kondo H., Uchikawa M., Juji T., Kumagai I. Highly efficient recovery of functional single-chain Fv fragments from inclusion bodies overexpressed in Escherichia coli by controlled introduction of oxidizing reagent-application to a human single-chain Fv fragment. J. Immunol. Meth. 1998, 219:119-129.
12. Hoogenboom H.R., de Bruïne A.P., Hufton S.E., Hoet R.M., Arends J.-W., Roovers R.C. Antibody phage display technology and its applications. Immunotechnology 1998, 4:1-20.
13. Kumada Y., Kuroki D., Yasui H., Ohse T., Kishimoto M. Characterization of polystyrene-binding peptides (PS-tags) for site-specific immobilization of proteins. J. Biosci. Bioeng. 2010, 109:583-587.
14. Kumada Y., Shiritani Y., Hamasaki K., Ohse T., Kisihmoto M. High biological activity of a recombinant protein immobilized onto polystyrene. Biotechnol. J. 2009, 4:1190-1197.
15. Kumada Y., Katoh S., Imanaka H., Imamura K., Nakanishi K. Development of a one-step ELISA method using an affinity peptide tag specific to a hydrophilic polystyrene surface. J. Biotechnol. 2007, 127:288-299.
16. Kumada Y., Zhao C., Ishimura R., Imanaka H., Imamura K., Nakanishi K. Protein-protein interaction analysis using an affinity peptide tag and hydrophilic polystyrene plate. J. Biotechnol. 2007, 128:354-361.
17. Kumada Y., Hamasaki K., Shiritani Y., Ohse T., Kisihmoto M. Efficient immobilization of a ligand antibody with high antigen-binding activity by use of a polystyrene binding peptide and an intelligent microtiter plate. J. Biotechnol. 2009, 142:135-141.
18. Kumada Y., Hamasaki K., Shiritani Y., Nakagawa A., Kuroki D., Ohse T., Choi D.H., Katakura Y., Kishimoto M. Direct immobilization of functional single-chain variable fragment antibodies (scFvs) onto a polystyrene plate by genetic fusion of a polystyrene-binding peptide (PS-tag). Anal. Bioanal. Chem. 2009, 395:759-765.
19. Kumada Y., Shiritani Y., Hamasaki K., Nakagawa A., Sasaki E., Kishimoto M. A novel solid-phase refolding method for preparation of scFv-immobilized polystyrene plates with high antigen-binding activity. Anal. Bioanal. Chem. 2010, 398:1295-1303.
20. Yoshida F., Ikeda A., Imakawa S., Miura Y. Oxygen absorption rates in stirred gas-liquid contactors condensed-complete copy for sale. Ind. Eng. Chem. 1960, 52:435-438.