Hydrogel embedding of precision-cut liver slices in a microfluidic device improves drug metabolic activity

Biotechnology and Bioengineering

Volumn 108, Issue 6, 2011, Pages 1404-1412

  van Midwoud, Paul M ^a   Merema, Marjolijn T ^a   Verweij, Niek ^a   Groothuis, Geny M M ^a   Verpoorte, Elisabeth ^a  

^a UNIVERSITY OF GRONINGEN   (Netherlands)

Author keywords

Matrigel; Metabolism; Microfluidics; Perifusion culture; Rat liver slices

Indexed keywords

7-ETHOXYCOUMARIN; 7-HYDROXYCOUMARIN; CALF SERUM; DEXAMETHASONES; DRUG METABOLISMS; DRUG-DRUG INTERACTIONS; ENZYME LEAKAGE; IN-VITRO; INCUBATION TIME; INITIAL VALUES; LACTATE DEHYDROGENASE; LIVER SLICES; MATRIGEL; METABOLIC ACTIVITY; METABOLIC RATES; MICRO-CHAMBERS; MICRO-FLUIDIC DEVICES; MICROFLOW SYSTEM; PERIFUSION CULTURE; PHASE I; PHASE II; PRECISION CUT; RAT LIVER SLICES; RAT LIVERS; WELL PLATES;

DRUG INTERACTIONS; HYDROGELS; LIVER; MICROFLUIDICS; PHYSIOLOGY;

METABOLISM;

7 ETHOXYCOUMARIN; DEXAMETHASONE; DIMETICONE; INSULIN; LACTATE DEHYDROGENASE; MATRIGEL; UMBELLIFERONE;

ANIMAL TISSUE; ARTICLE; BIOCHIP; CONTROLLED STUDY; DRUG METABOLISM; EMBEDDING; ENZYME ACTIVITY; HYDROGEL; LIVER METABOLISM; LIVER SLICE; MALE; METABOLIC RATE; MICROFLUIDICS; NONHUMAN; RAT; TISSUE CULTURE;

ANIMALS; EQUIPMENT DESIGN; HYDROGEL; LIVER; MALE; PHARMACEUTICAL PREPARATIONS; RATS; RATS, WISTAR; TISSUE ARRAY ANALYSIS;

RATTUS;

EID: 79954570688     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.23053     Document Type: Article

References (37)

1. Agius L, Chowdhury MH, Alberti KG. 1986. Regulation of ketogenesis, gluconeogenesis and the mitochondrial redox state by dexamethasone in hepatocyte monolayer-cultures. Biochem J 239(3): 593-601.
2. Arnold JT, Kaufman DG, Seppala M, Lessey BA. 2001. Endometrial stromal cells regulate epithelial cell growth in vitro: A new co-culture model. Hum Reprod 16(5): 836-845.
3. Brandon EFA, Raap CD, Meijerman I, Beijnen JH, Schellens JHM. 2003. An update on in vitro test methods in human hepatic drug biotransformation research: Pros and cons. Toxicol Appl Pharmacol 189(3): 233-246.
4. Buettner R, Straub RH, Ottinger I, Woenckhaus M, Scholmerich J, Bollheimer LC. 2005. Efficient analysis of hepatic glucose output and insulin action using a liver slice culture system. Horm Metab Res 37(3): 127-132.
5. Conway JG, Kauffman FC, Thurman RG. 1982. Rates of sulfation and glucuronidation of 7-hydroxycoumarin in periportal and pericentral regions of the liver lobule. Mol Pharmacol 22(2): 509-516.
6. De Graaf IAM, Groothuis GMM, Olinga P. 2007. Precision-cut tissue slices as a tool to predict metabolism of novel drugs. Expert Opin Drug Metab Toxicol 3(6): 879-898.
7. De Graaf IAM, Olinga P, De Jager MH, Merema MT, De Kanter R, Van de Kerkhof EG, Groothuis GMM. 2010. Preparation and incubation of precision-cut liver and intestinal slices for application in drug metabolism and toxicity studies. Nat Protoc 5(9): 1540-1551.
8. De Kanter R, De Jager MH, Draaisma AL, Jurva JU, Olinga P, Meijer DKF, Groothuis GMM. 2002. Drug-metabolizing activity of human and rat liver, lung, kidney and intestine slices. Xenobiotica 32(5): 349-362.
9. Dixit V, Arthur M, Reinhardt R, Gitnick G. 1992. Improved function of microencapsulated hepatocytes in a hybrid bioartificial liver support system. Artif Organs 16(4): 336-341.
10. Farkas D, Tannenbaum SR. 2005. In vitro methods to study chemically-induced hepatotoxicity: A literature review. Curr Drug Metab 6(2): 111-125.
11. Fisher RL, Ulreich JB, Nakazato PZ, Brendel K. 2001. Histological and biochemical evaluation of precision-cut liver slices. Toxicol Method 11(2): 59-79.
12. Hashemi E, Till C, Ioannides C. 1999. Stability of phase II conjugation systems in cultured precision-cut rat hepatic slices. Toxicol in Vitro 13(3): 459-466.
13. Hashemi E, Till C, Ioannides C. 2000. Stability of cytochrome P450 proteins in cultured precision-cut rat liver slices. Toxicology 149(2-3): 51-61.
14. Khong YM, Zhang J, Zhou SB, Cheung C, Doberstein K, Samper V, Yu H. 2007. Novel intra-tissue perfusion system for culturing thick liver tissue. Tissue Eng 13(9): 2345-2356.
15. Kibbey MC. 1994. Maintenance of the EHS sarcoma and Matrigel preparation. J Tissue Cult Meth 16(3-4): 227-230.
16. Kramer MA, Tracy TS. 2008. Studying cytochrome P450 kinetics in drug metabolism. Expert Opin Drug Metab Toxicol 4(5): 591-603.
17. Kuhn UD, Splinter FK, Rost M, Müller D. 1998. Induction of cytochrome P450 1A1 in rat liver slices by 7-ethoxycoumarin and 4-methyl-7-ethoxycoumarin. Exp Toxicol Pathol 50(4-6): 491-496.
18. LeCluyse EL, Bullock PL, Parkinson A. 1996. Strategies for restoration and maintenance of normal hepatic structure and function in long-term cultures of rat hepatocytes. Adv Drug Deliv Rev 22(1-2): 133-186.
19. Li WC, Ralphs KL, Slack JMW, Tosh D. 2007. Keratinocyte serum-free medium maintains long-term liver gene expression and function in cultured rat hepatocytes by preventing the loss of liver-enriched transcription factors. Int J Biochem Cell Biol 39(3): 541-554.
20. Liu L, LeCluyse EL, Liu J, Klaassen CD. 1996. Sulfotransferase gene expression in primary cultures of rat hepatocytes. Biochem Pharmacol 52(10): 1621-1630.
21. Naik RS, Mujumdar AM, Ghaskadbi S. 2004. Protection of liver cells from ethanol cytotoxicity by curcumin in liver slice culture in vitro. J Ethnopharmacol 95(1): 31-37.
22. Obatomi DK, Brant S, Anthonypillai V, Early DA, Bach PH. 1998. Optimizing preincubation conditions for precision-cut rat kidney and liver tissue slices: Effect of culture media and antioxidants. Toxicol in Vitro 12(6): 725-737.
23. Page JL, Johnson MC, Olsavsky KM, Strom SC, Zarbl H, Omiecinski CJ. 2007. Gene expression profiling of extracellular matrix as an effector of human hepatocyte phenotype in primary cell culture. Toxicol Sci 97(2): 384-397.
24. Pissowotzki K, Glöckner R, Müller D. 2003. Glucuronidation of 4-methylumbelliferone and 4-hydroxybiphenyl and in vitro induction of UDP-glucuronosyltransferase 2B12-mRNA in precision-cut rat liver slices. Exp Toxicol Pathol 54(5-6): 489-492.
25. Roberts RA, Ganey PE, Ju C, Kamendulis LM, Rusyn I, Klaunig JE. 2007. Role of the Kupffer cell in mediating hepatic toxicity and carcinogenesis. Toxicol Sci 96(1): 2-15.
26. Rogiers V, Vandenberghe Y, Callaerts A, Verleye G, Cornet M, Mertens K, Sonck W, Vercruysse A. 1990. Phase-I and phase-II xenobiotic biotransformation in cultures and cocultures of adult-rat hepatocytes. Biochem Pharmacol 40(8): 1701-1706.
27. Schuetz EG, Schuetz JD, May B, Guzelian PS. 1990. Regulation of cytochrome P-450b/e and P-450p gene-expression by growth-hormone in adult-rat hepatocytes cultured on a reconstituted basement-membrane. J Biol Chem 265(2): 1188-1192.
28. Sung JH, Shuler ML. 2009. A micro cell culture analog (μCCA) with 3-D hydrogel culture of multiple cell lines to assess metabolism-dependent cytotoxicity of anti-cancer drugs. Lab Chip 9(10): 1385-1394.
29. Toutain HJ, Moronvalle-Halley V, Sarsat JP, Chelin C, Hoet D, Leroy D. 1998. Morphological and functional integrity of precision-cut rat liver slices in rotating organ culture and multiwell plate culture: Effects of oxygen tension. Cell Biol Toxicol 14(3): 175-190.
30. Turncliff RZ, Meier PJ, Brouwer KLR. 2004. Effect of dexamethasone treatment on the expression and function of transport proteins in sandwich-cultured rat hepatocytes. Drug Metab Dispos 32(8): 834-839.
31. Van de Bovenkamp M, Groothuis GMM, Meijer DKF, Olinga P. 2008. Liver slices as a model to study fibrogenesis and test the effects of anti-fibrotic drugs on fibrogenic cells in human liver. Toxicol in Vitro 22(3): 771-778.
32. Van Midwoud PM, Groothuis GMM, Merema MT, Verpoorte E. 2010a. Microfluidic biochip for the perifusion of precision-cut rat liver slices for metabolism and toxicology studies. Biotechnol Bioeng 105(1): 184-194.
33. Van Midwoud PM, Merema MT, Verpoorte E, Groothuis GMM. 2010b. A microfluidic approach for in vitro assessment of interorgan interactions in drug metabolism using intestinal and liver slices. Anal Chem 83(1): 84-91.
34. Van Midwoud PM, Janssen GJ, Merema MT, De Graaf IAM, Groothuis GMM, Verpoorte E. 2011. On-line HPLC analysis system for metabolism and inhibition studies in precision-cut liver slices. Anal Chem 83(1): 84-91.
35. VandenBranden M, Wrighton SA, Ekins S, Gillespie JS, Binkley SN, Ring BJ, Gadberry MG, Mullins DC, Strom SC, Jensen CB. 1998. Alterations of the catalytic activities of drug-metabolizing enzymes in cultures of human liver slices. Drug Metab Dispos 26(11): 1063-1068.
36. Vickers AEM, Saulnier M, Cruz E, Merema MT, Rose K, Bentley P, Olinga P. 2004. Organ slice viability extended for pathway characterization: An in vitro model to investigate fibrosis. Toxicol Sci 82(2): 534-544.
37. Wortelboer HM, De Kruif CA, Van Iersel AAJ, Falke HE, Noordhoek J, Blaauboer BJ. 1990. The isoenzyme pattern of cytochrome P450 in rat hepatocytes in primary culture, comparing different enzyme-activities in microsomal incubations and intact monolayers. Biochem Pharmacol 40(11): 2525-2534.