Effect of constitutively active ras overexpression on cell growth in recombinant chinese hamster ovary cells

Biotechnology Progress

Volumn 27, Issue 2, 2011, Pages 577-580

  Kim, Yeon Gu ^a,b   Han, Young Kue ^a   Kim, Jee Yon ^a   Lee, Eun Gyo ^b   Lee, Hong Weon ^b   Lee, Gyun Min ^a  

^a Korea Advanced Institute of Science and Technology (KAIST)   (South Korea)

^b Korea Research Institute of Bioscience and Biotechnology (KRIBB)   (South Korea)

Author keywords

Cell growth; Erythropoietin; Inducible expression; Ras; RCHO cells

Indexed keywords

CELL LINES; CELL TYPES; CELLULAR RESPONSE; CHINESE HAMSTER OVARY; CHINESE HAMSTER OVARY CELLS; CONSTITUTIVELY ACTIVES; DOXYCYCLINE; ERYTHROPOIETIN; EXPRESSION LEVELS; INDUCIBLE EXPRESSION; LATE-EXPONENTIAL PHASE; MITOGEN ACTIVATED PROTEIN KINASE; OVER-EXPRESSION; PHOSPHOINOSITIDE 3-KINASE; RAS; RCHO CELLS; SIGNALING CASCADES; SIGNALING PATHWAYS; SPECIFIC GROWTH RATE; VIABLE CELLS;

CELL CULTURE; ENZYMES; GROWTH KINETICS; PIGMENTS; SIGNALING;

CELL GROWTH;

DOXYCYCLINE; ERYTHROPOIETIN; RAS PROTEIN;

ANIMAL; ARTICLE; CELL PROLIFERATION; CELL SURVIVAL; CHO CELL; CRICETULUS; DRUG EFFECT; GENETICS; HAMSTER; SIGNAL TRANSDUCTION;

ANIMALS; CELL PROLIFERATION; CELL SURVIVAL; CHO CELLS; CRICETINAE; CRICETULUS; DOXYCYCLINE; ERYTHROPOIETIN; MAP KINASE SIGNALING SYSTEM; RAS PROTEINS; SIGNAL TRANSDUCTION;

CRICETULUS GRISEUS;

EID: 79954415962     PISSN: 87567938     EISSN: 15206033     Source Type: Journal    
DOI: 10.1002/btpr.567     Document Type: Article

References (23)

1. Arden N, Betenbaugh MJ. Life and death in mammalian cell culture: strategies for apoptosis inhibition. Trends Biotechnol. 2004; 22: 174-180.
2. Mohan C, Kim YG, Koo J, Lee GM. Assessment of cell engineering strategies for improved therapeutic protein production in CHO cells. Biotechnol J. 2008; 3: 624-630.
3. Arden N, Ahn SH, Vaz W, Rhodes M, Hancock C, Abitorabi A, Betenbaugh MJ. Chemical caspase inhibitors enhance cell culture viabilities and protein titer. Biotechnol Prog. 2007; 23: 506-511.
4. Morris AE, Schmid J. Effects of insulin and LongR(3) on serum-free Chinese hamster ovary cell cultures expressing two recombinant proteins. Biotechnol Prog. 2000; 16: 693-697.
5. Sunstrom NA, Gay RD, Wong DC, Kitchen NA, DeBoer L, Gray PP. Insulin-like growth factor-I and transferrin mediate growth and survival of Chinese hamster ovary cells. Biotechnol Prog. 2000; 16: 698-702.
6. Chiang GG, Sisk WP. Bcl-x(L) mediates increased production of humanized monoclonal antibodies in Chinese hamster ovary cells. Biotechnol Bioeng. 2005; 91: 779-792.
7. Kim YG, Kim JY, Mohan C, Lee GM. Effect of Bcl-xL overexpression on apoptosis and autophagy in recombinant Chinese hamster ovary cells under nutrient-deprived condition. Biotechnol Bioeng. 2009; 103: 757-766.
8. Tey BT, Singh RP, Piredda L, Piacentini M, Al-Rubeai M. Influence of bcl-2 on cell death during the cultivation of a Chinese hamster ovary cell line expressing a chimeric antibody. Biotechnol Bioeng. 2000; 68: 31-43.
9. Ifandi V, Al-Rubeai M. Regulation of cell proliferation and apoptosis in CHO-K1 cells by the coexpression of c-Myc and Bcl-2. Biotechnol Prog. 2005; 216: 671-677.
10. Majors BS, Arden N, Oyler GA, Chiang GG, Pederson NE, Betenbaugh MJ. E2F-1 overexpression increases viable cell density in batch cultures of Chinese hamster ovary cells. J Biotechnol. 2008; 138: 103-106.
11. Renner WA, Lee KH, Hatzimanikatis V, Bailey JE, Eppenberger HM. Recombinant cyclin E expression activates proliferation and obviates surface attachment of Chinese hamster ovary (CHO) cells in protein-free medium. Biotechnol Bioeng. 1995; 47: 476-482.
12. Shields JM, Pruitt K, McFall A, Shaub A, Der CJ. Understanding Ras: 'it ain't over 'til it's over'. Trends Cell Biol. 2000; 10: 147-154.
13. Scheele JS, Rhee JM, Boss GR. Determination of absolute amounts of GDP and GTP bound to Ras in mammalian cells: comparison of parental and Ras-overproducing NIH 3T3 fibroblasts. Proc Natl Acad Sci USA. 1995; 92: 1097-1100.
14. Karnoub AE, Weinberg RA. Ras oncogenes: split personalities. Nat Rev Mol Cell Biol. 2008; 9: 517-531.
15. Fukuda S, Pelus LM. Activated H-Ras regulates hematopoietic cell survival by modulating Survivin. Biochem Biophys Res Commun. 2004; 323: 636-644.
16. Khosravi-Far R, White MA, Westwick JK, Solski PA, Chrzanowska-Wodnicka M, Van Aelst L, Wigler MH, Der CJ. Oncogenic Ras activation of Raf/mitogen-activated protein kinase-independent pathways is sufficient to cause tumorigenic transformation. Mol Cell Biol. 1996; 16: 3923-3933.
17. Shao J, Sheng H, DuBois RN, Beauchamp RD. Oncogenic Ras-mediated cell growth arrest and apoptosis are associated with increased ubiquitin-dependent cyclin D1 degradation. J Biol Chem. 2000; 275: 22916-22924.
18. Kim SH, Lee GM. Development of serum-free medium supplemented with hydrolysates for the production of therapeutic antibodies in CHO cell cultures using design of experiments. Appl Microbiol Biotechnol. 2009; 83: 639-648.
19. Rubio I. Use of the Ras binding domain of c-Raf for biochemical and live-cell analysis of Ras activation. Biochem Soc Trans. 2005; 33: 662-663.
20. Mazur X, Eppenberger HM, Bailey JE, Fussenegger M. A novel autoregulated proliferation-controlled production process using recombinant CHO cells. Biotechnol Bioeng. 1999; 65: 144-150.
21. Hwang SO, Lee GM. Effect of Akt overexpression on programmed cell death in antibody-producing Chinese hamster ovary cells. J Biotechnol. 2009; 139: 89-94.
22. Pratsinis H, Kletsas D. PDGF, bFGF and IGF-I stimulate the proliferation of intervertebral disc cells in vitro via the activation of the ERK and Akt signaling pathways. Eur Spine J. 2007; 16: 1858-1866.
23. Tsakiridis T, Tsiani E, Lekas P, Bergman A, Cherepanov V, Whiteside C, Downey GP. Insulin, insulin-like growth factor-I, and platelet-derived growth factor activate extracellular signal-regulated kinase by distinct pathways in muscle cells. Biochem Biophys Res Commun. 2001; 288: 205-211.