Depolarization after resonance energy transfer (DARET): A sensitive fluorescence-based assay for botulinum neurotoxin protease activity

Analytical Biochemistry

Volumn 413, Issue 1, 2011, Pages 36-42

  Gilmore, Marcella A ^a   Williams, Dudley ^a   Okawa, Yumiko ^a   Holguin, Bret ^a   James, Nicholas G ^b   Ross, Justin A ^b   Roger Aoki, K ^a   Jameson, David M ^b   Steward, Lance E ^a  

^a ALLERGAN INC   (United States)

^b UNIVERSITY OF HAWAII   (United States)

Author keywords

BFP; Botulinum neurotoxin; DARET; Fluorescence polarization; FRET; GFP; SNAP 25

Indexed keywords

ENERGY TRANSFER; FORSTER RESONANCE ENERGY TRANSFER; POLARIZATION; PROTEINS; RATE CONSTANTS; RESONANCE;

BOTULINUM NEUROTOXINS; DARET; FLUORESCENCE POLARIZATION; FRET; SNAP-25;

DEPOLARIZATION;

BLUE FLUORESCENT PROTEIN; BOTULINUM TOXIN A; GREEN FLUORESCENT PROTEIN; PROTEIN; PROTEINASE; UNCLASSIFIED DRUG;

ARTICLE; CONTROLLED STUDY; DEPOLARIZATION; DEPOLARIZATION AFTER RESONANCE ENERGY TRANSFER; ENERGY TRANSFER; ENZYME ACTIVITY; FLUORESCENCE; KINETICS; LIGHT; POLARIZATION; PRIORITY JOURNAL; PROTEIN CLEAVAGE; PROTEIN DEGRADATION;

EID: 79953722208     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2011.01.043     Document Type: Article

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