Detection and quantification of botulinum neurotoxin type A by a novel rapid in vitro fluorimetric assay

Applied and Environmental Microbiology

Volumn 75, Issue 13, 2009, Pages 4382-4390

  Poras, Hervé ^a   Ouimet, Tanja ^a   Orng, Sou Vinh ^a   Fournié Zaluski, Marie Claude ^a   Popoff, Michel R ^b   Roques, Bernard P ^a  

^a Pharmaleads   (France)

^b INSTITUT PASTEUR   (France)

Author keywords

[No Author keywords available]

Indexed keywords

BIOTERRORISM AGENT; BOTULINUM NEUROTOXIN TYPE A; C TERMINUS; CATALYTIC EFFICIENCIES; ENZYMATIC ASSAY; FLUORESCENCE EMISSION; FLUORIMETRIC ASSAYS; IN-VITRO; LETHAL DOSE; LOW DOSE; NEUROMUSCULAR JUNCTIONS; NORLEUCINE; PEPTIDE SEQUENCES; POISONOUS SUBSTANCES; THERAPEUTIC AGENTS;

AMINES; AMINO ACIDS; FLUORESCENCE; ORGANIC ACIDS;

SUBSTRATES;

4 NITROPHENYLALANINE; ACETYLCHOLINE; ALANINE DERIVATIVE; BOTULINUM TOXIN A; METHIONINE; NORLEUCINE; PHENYLALANINE DERIVATIVE; PYRENYLALANINE; SYNAPTOSOMAL ASSOCIATED PROTEIN 25; UNCLASSIFIED DRUG;

BACTERIUM; BIOASSAY; BIOTERRORISM; CALIBRATION; DETECTION METHOD; ENZYME ACTIVITY; FLUOROMETRY; METABOLITE; OPTIMIZATION; ORGANIC COMPOUND; PEPTIDE; PROTEIN; QUANTITATIVE ANALYSIS; TOXIN;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; BINDING AFFINITY; BIOLOGICAL WARFARE; CARBOXY TERMINAL SEQUENCE; CATALYSIS; ENZYMATIC ASSAY; FLACCID PARALYSIS; FLUORESCENCE; FLUOROMETRY; IN VITRO STUDY; NEUROMUSCULAR SYNAPSE; NONHUMAN; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN CLEAVAGE; PROTEIN SYNTHESIS; QUANTITATIVE ANALYSIS; WRINKLE;

ANIMALS; BOTULINUM TOXIN TYPE A; FLUORESCENT DYES; FLUOROMETRY; MICE; SENSITIVITY AND SPECIFICITY;

EID: 67649582935     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.00091-09     Document Type: Article

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