Improved production of human type II procollagen in the yeast Pichia pastoris in shake flasks by a wireless-controlled fed-batch system

BMC Biotechnology

Volumn 8, Issue , 2008, Pages

  Ruottinen, Maria ^a   Bollok, Monika ^a   Kögler, Martin ^a   Neubauer, Antje ^a   Krause, Mirja ^a   Hämäläinen, Eija Riitta ^a   Myllyharju, Johanna ^a   Vasala, Antti ^a   Neubauer, Peter ^a  

^a UNIVERSITY OF OULU   (Finland)

Author keywords

[No Author keywords available]

Indexed keywords

COLLAGEN; FERMENTATION; GENE EXPRESSION; PROTEINS; YEAST;

FEEDING PROTOCOLS; PROCOLLAGEN; RECOMBINANT PROTEINS; SHAKE FLASKS; WIRELESS FEEDING UNIT;

BIOTECHNOLOGY;

ALCOHOL OXIDASE; FORMALDEHYDE DEHYDROGENASE; MESSENGER RNA; METHANOL; OXYGEN; PROCOLLAGEN; PROCOLLAGEN PROLINE 2 OXOGLUTARATE 4 DIOXYGENASE; RECOMBINANT PROTEIN; COLLAGEN TYPE 2;

ARTICLE; BIOREACTOR; COLLAGEN SYNTHESIS; COMPUTER SYSTEM; CONTROLLED STUDY; ENZYME ACTIVITY; FED BATCH FERMENTATION; FINANCIAL MANAGEMENT; GENE EXPRESSION REGULATION; NONHUMAN; ONLINE MONITORING; OXYGEN TENSION; PICHIA PASTORIS; PROCESS OPTIMIZATION; PROMOTER REGION; PROTEIN DEGRADATION; PROTEIN SYNTHESIS; STARVATION; CULTURE TECHNIQUE; ENZYMOLOGY; EQUIPMENT; EQUIPMENT DESIGN; FLOW INJECTION ANALYSIS; GENETICS; HUMAN; INSTRUMENTATION; METABOLISM; MICROBIOLOGY; ONLINE SYSTEM; PICHIA; PROTEIN ENGINEERING; TELEMETRY;

PICHIA PASTORIS;

BIOREACTORS; CELL CULTURE TECHNIQUES; COLLAGEN TYPE II; EQUIPMENT DESIGN; EQUIPMENT FAILURE ANALYSIS; FLOW INJECTION ANALYSIS; HUMANS; ONLINE SYSTEMS; OXYGEN; PICHIA; PROTEIN ENGINEERING; TELEMETRY;

EID: 42249089188     PISSN: None     EISSN: 14726750     Source Type: Journal    
DOI: 10.1186/1472-6750-8-33     Document Type: Article

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