Characterization of a newly identified mycobacterial tautomerase with promiscuous dehalogenase and hydratase activities reveals a functional link to a recently diverged cis -3-chloroacrylic acid dehalogenase

Biochemistry

Volumn 50, Issue 14, 2011, Pages 2889-2899

  Baas, Bert Jan ^a   Zandvoort, Ellen ^a   Wasiel, Anna A ^a   Quax, Wim J ^a   Poelarends, Gerrit J ^a  

^a UNIVERSITY OF GRONINGEN   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

20TH CENTURY; ACTIVE SITE; BACTERIAL PATHWAY; COVALENT MODIFICATIONS; DEHALOGENASE; DIVERGENT EVOLUTION; FUNCTIONAL LINKS; HOMOLOGOUS PROTEINS; HYDRATASE; HYDRATASE ACTIVITY; MICHAEL-TYPE REACTION; MYCOBACTERIAL; SPECTROSCOPIC STUDIES; STRUCTURAL FEATURE; TAUTOMERASE;

AMINO ACIDS; ENZYMES; NUCLEOPHILES; SPECTROSCOPIC ANALYSIS;

CRYSTAL STRUCTURE;

3 CHLOROACRYLIC ACID DEHALOGENASE; BACTERIAL ENZYME; HYDROLYASE; NUCLEOPHILE; PYRUVIC ACID DERIVATIVE; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; CHEMICAL MODIFICATION; CHEMICAL REACTION KINETICS; CONTROLLED STUDY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME INACTIVATION; ENZYME STRUCTURE; MICHAEL ADDITION; MYCOBACTERIUM; NONHUMAN; NUCLEOTIDE SEQUENCE; PH; PRIORITY JOURNAL; PROTON NUCLEAR MAGNETIC RESONANCE;

ACRYLATES; AMINO ACID SEQUENCE; BACTERIAL PROTEINS; BINDING SITES; BIOCATALYSIS; CATALYTIC DOMAIN; FATTY ACIDS, UNSATURATED; HYDRO-LYASES; HYDROLASES; INTRAMOLECULAR OXIDOREDUCTASES; KINETICS; MAGNETIC RESONANCE SPECTROSCOPY; MODELS, CHEMICAL; MOLECULAR SEQUENCE DATA; MOLECULAR STRUCTURE; MYCOBACTERIUM SMEGMATIS; SEQUENCE HOMOLOGY, AMINO ACID; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; STEREOISOMERISM; SUBSTRATE SPECIFICITY;

BACTERIA (MICROORGANISMS);

EID: 79953678988     PISSN: 00062960     EISSN: 15204995     Source Type: Journal    
DOI: 10.1021/bi200071k     Document Type: Article

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