Crystal structures of native and inactivated cis-3-chloroacrylic acid dehalogenase: Structural basis for substrate specificity and inactivation by (R)-oxirane-2-carboxylate

Journal of Biological Chemistry

Volumn 282, Issue 4, 2007, Pages 2440-2449

  De Jong, René M ^a   Bazzacco, Paola ^a   Poelarends, Gerrit J ^a   Johnson Jr , William H ^b   Yoon, Jae Kim ^b   Burks, Elizabeth A ^b   Serrano, Hector ^b   Thunnissen, Andy Mark W H ^a   Whitman, Christian P ^b   Dijkstra, Bauke W ^a  

^a UNIVERSITY OF GRONINGEN   (Netherlands)

^b UNIVERSITY OF TEXAS AT AUSTIN   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CARBOXYLATE GROUP; CATALYTIC RESIDUES; GENE DUPLICATION;

BIOCHEMISTRY; CRYSTAL STRUCTURE; DEGRADATION; DEHALOGENATION; ENANTIOMERS; GENES;

ENZYMES;

3 CHLOROACRYLIC ACID DEHALOGENASE; ARGININE; CARBOXYLIC ACID DERIVATIVE; ENZYME; EPOXIDE; ETHYLENE OXIDE DERIVATIVE; GLUTAMIC ACID; HISTIDINE; LACTIC ACID; MUTANT PROTEIN; NITROGEN; OXIRANE 2 CARBOXYLATE; PROLINE; TYROSINE; UNCLASSIFIED DRUG; WATER; CIS 3 CHLOROACRYLIC ACID DEHALOGENASE; CIS-3-CHLOROACRYLIC ACID DEHALOGENASE; ETHYLENE OXIDE; HYDROLASE;

ARTICLE; ATOM; BINDING SITE; CATALYSIS; CIS ISOMER; CRYSTAL STRUCTURE; ENANTIOMER; ENZYME ACTIVITY; ENZYME INACTIVATION; ENZYME MECHANISM; ENZYME MODIFICATION; ENZYME SPECIFICITY; ENZYME STRUCTURE; ENZYME SUBSTRATE; ENZYME SUBSTRATE COMPLEX; POLARIZATION; PRIORITY JOURNAL; PROTON TRANSPORT; RING OPENING; X RAY CRYSTALLOGRAPHY; AMINO ACID SEQUENCE; CHEMICAL MODEL; CHEMICAL STRUCTURE; CHEMISTRY; ENZYME ACTIVATION; GENETICS; KINETICS; METABOLISM; MOLECULAR GENETICS; PSEUDOMONAS; SITE DIRECTED MUTAGENESIS; STRUCTURE ACTIVITY RELATION;

BACTERIA (MICROORGANISMS);

AMINO ACID SEQUENCE; BINDING SITES; CRYSTALLOGRAPHY, X-RAY; ENZYME ACTIVATION; EPOXY COMPOUNDS; ETHYLENE OXIDE; HYDROLASES; KINETICS; MODELS, CHEMICAL; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PSEUDOMONAS; STRUCTURE-ACTIVITY RELATIONSHIP; SUBSTRATE SPECIFICITY;

EID: 34047269260     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M608134200     Document Type: Article

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