CDNA cloning of porcine PKD2 gene and RNA interference in LLC-PK1 cells

Gene

Volumn 476, Issue 1-2, 2011, Pages 38-45

  Wang, Qingsong ^a,b   Yin, Haifang ^a   He, Jin ^b   Ye, Jianhua ^b   Ding, Fangrong ^b   Wang, Shaohua ^b   Hu, Xiaoxiang ^b   Meng, Qingyong ^b   Li, Ning ^b  

^a TIANJIN MEDICAL UNIVERSITY   (China)

^b CHINA AGRICULTURAL UNIVERSITY   (China)

Author keywords

ADPKD; Cell proliferation; Knockdown; Polycystin 2; TRPP2

Indexed keywords

COMPLEMENTARY DNA; SHORT HAIRPIN RNA; SMALL INTERFERING RNA;

ANIMAL CELL; ARTICLE; CELL STRAIN; CONTROLLED STUDY; EXON; GENE; GENE EXPRESSION; GENE FUNCTION; GENE STRUCTURE; GENE TARGETING; HUMAN; HUMAN CELL; KIDNEY POLYCYSTIC DISEASE; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PKD2 GENE; PRIORITY JOURNAL; RNA INTERFERENCE; SWINE;

ANIMALS; BASE SEQUENCE; CELL CYCLE; CLONING, MOLECULAR; DISEASE MODELS, ANIMAL; DNA PRIMERS; DNA, COMPLEMENTARY; GENE EXPRESSION PROFILING; GENE KNOCKDOWN TECHNIQUES; HELA CELLS; HUMANS; KIDNEY; LLC-PK1 CELLS; POLYCYSTIC KIDNEY, AUTOSOMAL DOMINANT; RNA INTERFERENCE; RNA, SMALL INTERFERING; SWINE; TISSUE DISTRIBUTION; TRPP CATION CHANNELS;

ANIMALIA; SUIDAE; SUS;

EID: 79953296409     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.gene.2011.01.017     Document Type: Article

References (49)

1. Bachner L., Vinet M.C., Lacave R., Babron M.C., Rondeau E., Sraer J.D., et al. Linkage study of a large family with autosomal dominant polycystic kidney disease with reduced expression. Absence of linkage to the PKD 1 locus. Hum. Genet. 1990, 85:221-227.
2. Bai C.X., Giamarchi A., Rodat-Despoix L., Padilla F., Downs T., Tsiokas L., et al. Formation of a new receptor-operated channel by heteromeric assembly of TRPP2 and TRPC1 subunits. EMBO Rep. 2008, 9:472-479.
3. Bhunia A.K., Piontek K., Boletta A., Liu L., Qian F., Xu P.N., et al. PKD1 induces p21(waf1) and regulation of the cell cycle via direct activation of the JAK-STAT signaling pathway in a process requiring PKD2. Cell 2002, 109:157-168.
4. Brummelkamp T.R., Bernards R., Agami R. Stable suppression of tumorigenicity by virus-mediated RNA interference. Cancer Cell 2002, 2:243-247.
5. Brummelkamp T.R., Bernards R., Agami R. A system for stable expression of short interfering RNAs in mammalian cells. Science 2002, 296:550-553.
6. Chapman A.B., Guay-Woodford L.M., Grantham J.J., Torres V.E., Bae K.T., Baumgarten D.A., et al. Renal structure in early autosomal-dominant polycystic kidney disease (ADPKD): the Consortium for Radiologic Imaging Studies of Polycystic Kidney Disease (CRISP) cohort. Kidney Int. 2003, 64:1035-1045.
7. Chen X.Z., Li Q., Wu Y., Liang G., Lara C.J., Cantiello H.F. Submembraneous microtubule cytoskeleton: interaction of TRPP2 with the cell cytoskeleton. FEBS J. 2008, 275:4675-4683.
8. Daoust M.C., Reynolds D.M., Bichet D.G., Somlo S. Evidence for a third genetic locus for autosomal dominant polycystic kidney disease. Genomics 1995, 25:733-736.
9. Felekkis K.N., Koupepidou P., Kastanos E., Witzgall R., Bai C.X., Li L., et al. Mutant polycystin-2 induces proliferation in primary rat tubular epithelial cells in a STAT-1/p21-independent fashion accompanied instead by alterations in expression of p57KIP2 and Cdk2. BMC Nephrol. 2008, 9:10.
10. Gabow P.A. Autosomal dominant polycystic kidney disease. Am. J. Kidney Dis. 1993, 22:511-512.
11. Giering J.C., Grimm D., Storm T.A., Kay M.A. Expression of shRNA from a tissue-specific pol II promoter is an effective and safe RNAi therapeutic. Mol. Ther. 2008, 16:1630-1636.
12. Gonzalez-Perrett S., Kim K., Ibarra C., Damiano A.E., Zotta E., Batelli M., et al. Polycystin-2, the protein mutated in autosomal dominant polycystic kidney disease (ADPKD), is a Ca2+-permeable nonselective cation channel. Proc. Natl Acad. Sci. USA 2001, 98:1182-1187.
13. Grantham J.J. CRISP: opening a new frontier in the diagnosis and treatment of PKD. Nephrol. News Issues 2006, 20:29-30.
14. Hannon G.J. RNA interference. Nature 2002, 418:244-251.
15. Hohjoh H. RNA interference (RNA(i)) induction with various types of synthetic oligonucleotide duplexes in cultured human cells. FEBS Lett. 2002, 521:195-199.
16. Huang X., Madan A. CAP3: a DNA sequence assembly program. Genome Res. 1999, 9:868-877.
17. Jackson L.N., Li J., Chen L.A., Townsend C.M., Evers B.M. Overexpression of wild-type PKD2 leads to increased proliferation and invasion of BON endocrine cells. Biochem. Biophys. Res. Commun. 2006, 348:945-949.
18. Kaariainen H. Polycystic kidney disease in children: a genetic and epidemiological study of 82 Finnish patients. J. Med. Genet. 1987, 24:474-481.
19. Kimberling W.J., Kumar S., Gabow P.A., Kenyon J.B., Connolly C.J., Somlo S. Autosomal dominant polycystic kidney disease: localization of the second gene to chromosome 4q13-q23. Genomics 1993, 18:467-472.
20. Kottgen M., Buchholz B., Garcia-Gonzalez M.A., Kotsis F., Fu X., Doerken M., et al. TRPP2 and TRPV4 form a polymodal sensory channel complex. J. Cell Biol. 2008, 182:437-447.
21. Koulen P., Cai Y., Geng L., Maeda Y., Nishimura S., Witzgall R., et al. Polycystin-2 is an intracellular calcium release channel. Nat. Cell Biol. 2002, 4:191-197.
22. Li Q., Montalbetti N., Shen P.Y., Dai X.Q., Cheeseman C.I., Karpinski E., et al. Alpha-actinin associates with polycystin-2 and regulates its channel activity. Hum. Mol. Genet. 2005, 14:1587-1603.
23. Li X., Luo Y., Starremans P.G., McNamara C.A., Pei Y., Zhou J. Polycystin-1 and polycystin-2 regulate the cell cycle through the helix-loop-helix inhibitor Id2. Nat. Cell Biol. 2005, 7:1202-1212.
24. Li Y., Wright J.M., Qian F., Germino G.G., Guggino W.B. Polycystin 2 interacts with type I inositol 1, 4, 5-trisphosphate receptor to modulate intracellular Ca2+ signaling. J. Biol. Chem. 2005, 280:41298-41306.
25. Liang G., Li Q., Tang Y., Kokame K., Kikuchi T., Wu G., et al. Polycystin-2 is regulated by endoplasmic reticulum-associated degradation. Hum. Mol. Genet. 2008, 17:1109-1119.
26. Liang G., Yang J., Wang Z., Li Q., Tang Y., Chen X.Z. Polycystin-2 down-regulates cell proliferation via promoting PERK-dependent phosphorylation of eIF2alpha. Hum. Mol. Genet. 2008, 17:3254-3262.
27. Livak K.J., Schmittgen T.D. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods 2001, 25:402-408.
28. Magistroni R., He N., Wang K., Andrew R., Johnson A., Gabow P., et al. Genotype-renal function correlation in type 2 autosomal dominant polycystic kidney disease. J. Am. Soc. Nephrol. 2003, 14:1164-1174.
29. Mochizuki T., Wu G., Hayashi T., Xenophontos S.L., Veldhuisen B., Saris J.J., et al. PKD2, a gene for polycystic kidney disease that encodes an integral membrane protein. Science 1996, 272:1339-1342.
30. Newby L.J., Streets A.J., Zhao Y., Harris P.C., Ward C.J., Ong A.C. Identification, characterization, and localization of a novel kidney polycystin-1-polycystin-2 complex. J. Biol. Chem. 2002, 277:20763-20773.
31. Perantoni A., Berman J.J. Properties of Wilms' tumor line (TuWi) and pig kidney line (LLC-PK1) typical of normal kidney tubular epithelium. In Vitro 1979, 15:446-454.
32. Pignatelli P.M., Pound S.E., Carothers A.D., Macnicol A.M., Allan P.L., Watson M.L., et al. Multipoint mapping of adult onset polycystic kidney disease (PKD1) on chromosome 16. J. Med. Genet. 1992, 29:638-641.
33. Pinkenburg O., Platz J., Beisswenger C., Vogelmeier C., Bals R. Inhibition of NF-kappaB mediated inflammation by siRNA expressed by recombinant adeno-associated virus. J. Virol. Meth. 2004, 120:119-122.
34. Qiu S., Adema C.M., Lane T. A computational study of off-target effects of RNA interference. Nucleic Acids Res. 2005, 33:1834-1847.
35. Reeders S.T., Breuning M.H., Davies K.E., Nicholls R.D., Jarman A.P., Higgs D.R., et al. A highly polymorphic DNA marker linked to adult polycystic kidney disease on chromosome 16. Nature 1985, 317:542-544.
36. Rossetti S., Chauveau D., Kubly V., Slezak J.M., Saggar-Malik A.K., Pei Y., et al. Association of mutation position in polycystic kidney disease 1 (PKD1) gene and development of a vascular phenotype. Lancet 2003, 361:2196-2201.
37. Rubinson D.A., Dillon C.P., Kwiatkowski A.V., Sievers C., Yang L., Kopinja J., et al. A lentivirus-based system to functionally silence genes in primary mammalian cells, stem cells and transgenic mice by RNA interference. Nat. Genet. 2003, 33:401-406.
38. Rundle D.R., Gorbsky G., Tsiokas L. PKD2 interacts and co-localizes with mDia1 to mitotic spindles of dividing cells: role of mDia1 IN PKD2 localization to mitotic spindles. J. Biol. Chem. 2004, 279:29728-29739.
39. Saayman S., Barichievy S., Capovilla A., Morris K.V., Arbuthnot P., Weinberg M.S. The efficacy of generating three independent anti-HIV-1 siRNAs from a single U6 RNA Pol III-expressed long hairpin RNA. PLoS ONE 2008, 3:e2602.
40. Sharp P.A., Zamore P.D. Molecular biology. RNA interference. Science 2000, 287:2431-2433.
41. Stegmeier F., Hu G., Rickles R.J., Hannon G.J., Elledge S.J. A lentiviral microRNA-based system for single-copy polymerase II-regulated RNA interference in mammalian cells. Proc. Natl Acad. Sci. USA 2005, 102:13212-13217.
42. Torres V.E., Cai Y., Chen X., Wu G.Q., Geng L., Cleghorn K.A., et al. Vascular expression of polycystin-2. J. Am. Soc. Nephrol. 2001, 12:1-9.
43. Torres V.E., Harris P.C., Pirson Y. Autosomal dominant polycystic kidney disease. Lancet 2007, 369:1287-1301.
44. Tsiokas L., Arnould T., Zhu C., Kim E., Walz G., Sukhatme V.P. Specific association of the gene product of PKD2 with the TRPC1 channel. Proc. Natl Acad. Sci. USA 1999, 96:3934-3939.
45. Tuschl T. RNA interference and small interfering RNAs. Chembiochem 2001, 2:239-245.
46. Vassilev P.M., Guo L., Chen X.Z., Segal Y., Peng J.B., Basora N., et al. Polycystin-2 is a novel cation channel implicated in defective intracellular Ca(2+) homeostasis in polycystic kidney disease. Biochem. Biophys. Res. Commun. 2001, 282:341-350.
47. Yang D., Goga A., Bishop J.M. RNA interference (RNAi) with RNase III-prepared siRNAs. Meth. Mol. Biol. 2004, 252:471-482.
48. Zhang P., Luo Y., Chasan B., Gonzalez-Perrett S., Montalbetti N., Timpanaro G.A., et al. The multimeric structure of polycystin-2 (TRPP2): structural-functional correlates of homo- and hetero-multimers with TRPC1. Hum. Mol. Genet. 2009, 18:1238-1251.
49. Zhao L.J., Jian H., Zhu H. Specific gene inhibition by adenovirus-mediated expression of small interfering RNA. Gene 2003, 316:137-141.